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ProteomicsA novel class of CLK kinases inhibitors that target SRSF10 and promote p53mediated cytotoxicity on human colorectal cancer cells
The elevated expression of the splicing regulator SRSF10 in metastatic colorectal cancer (CRC) stimulates the production of the pro-tumorigenic Bclaf1-L splice variant. We discovered a group of small molecules with an aminothiazole carboxamide core (GPS167, GPS192 and others) that decrease productio...
ORGANISM(S): Homo sapiens (Human) 
2021-09-09 | PXD022333 | Pride
Clk kinases inhibitor Srsf10 Human colorectal cancer cells 
GenomicsTCERG1 regulates alternative splicing of Bcl-x gene by modulating the rate of RNAPII transcription
Complex functional coupling exists between transcriptional elongation and pre-mRNA alternative splicing. Pausing sites and changes in the rate of transcription by RNAPII may therefore have a fundamental impact in the regulation of alternative splicing. Here, we show that the elongation and splicing-...
ORGANISM(S): Homo sapiens 
2012-01-12 | E-GEOD-33716 | biostudies-arrayexpress
ProteomicsR2TP_BioID-MS - R2TP/Prefoldin-like component RUVBL1/RUVBL2 directly interacts with ZNHIT2 to regulate assembly of U5 small nuclear ribonucleoprotein
The R2TP/Prefoldin-like (R2TP/PFDL) complex is a cochaperone complex involved in the assembly of a number of critical protein complexes. Here we use proximity-dependent biotinylation (BioID) coupled to mass spectrometry to confirm interactions of ZNHIT2, a cofactor of R2TP/PFDN, identified by TAP-MS...
ORGANISM(S): Homo sapiens (Human) 
2018-10-24 | PXD006200 | Pride
Human Lc-msms U5 snrnp Flp-in t-rex hek 293 Znhit2 R2tp/pfdn Bioid 
ProteomicsR2TP_SILAC_FLAG-MS - R2TP/Prefoldin-like component RUVBL1/RUVBL2 directly interacts with ZNHIT2 to regulate assembly of U5 small nuclear ribonucleoprotein
The R2TP/Prefoldin-like (R2TP/PFDL) complex is a cochaperone complex involved in the assembly of a number of critical protein complexes. Here we use stable isotope labeling with amino acids in cell culture (SILAC) and FLAG tag affinity purification coupled to mass spectrometry to assess whether siRN...
ORGANISM(S): Homo sapiens (Human) 
2017-11-02 | PXD006199 | Pride
Human Lc-msms Tsc complex Affinity purification U5 snrnp Znhit2 Hek 293 Flag 
ProteomicsR2TP_TAP-MS - R2TP/Prefoldin-like component RUVBL1/RUVBL2 directly interacts with ZNHIT2 to regulate assembly of U5 small nuclear ribonucleoprotein
The R2TP/Prefoldin-like (R2TP/PFDL) complex is a cochaperone complex involved in the assembly of a number of critical protein complexes. Here we use multiple target tandem affinity purification (TAP) coupled to mass spectrometry to map out the interaction network around R2TP/PFDL.
ORGANISM(S): Homo sapiens (Human) 
2018-10-24 | PXD006198 | Pride
Human Lc-msms Tap Hek 293 
ProteomicsThe unphosphorylated form of the PAQosome core subunit RPAP3 binds ribosomal preassembly complexes to modulate ribosome biogenesis.
The PAQosome (Particle for Arrangement of Quaternary structure) is a twelve-subunit HSP90 co-chaperone involved in the biogenesis of several human protein complexes. Two mechanisms of client selection have previously been identified, namely the selective recruitment of specific adaptors and the diff...
ORGANISM(S): Homo sapiens (Human) 
2022-06-27 | PXD026647 | Pride
Ap-ms Ribosome preassembly factors Rpap3 Ribosome biogenesis Chaperone Ck2 phosphorylation Paqosome 
TranscriptomicsA function for the hnRNP A1/A2 proteins in transcription elongation
The hnRNP A1 and A2 proteins regulate processes such as alternative pre-mRNA splicing and mRNA stability. Here, we report that a reduction in the levels of hnRNP A1 and A2 by RNA interference or their cytoplasmic retention by osmotic stress drastically increases the transcription of a reporter gene....
ORGANISM(S): Homo sapiens 
2015-04-07 | E-GEOD-67617 | biostudies-arrayexpress
TranscriptomicsInvestigation into human Tra2 protein-dependent splicing in MDA-MB-231 cells using iCLIP and RNA-seq
Alternative splicing—the production of multiple mRNA isoforms from a single gene—is regulated in part by RNA-binding proteins (RBPs). While the RBPs Tra2? and Tra2? have both been implicated in the regulation of alternative splicing, their relative contribution to this process are not well understoo...
ORGANISM(S): Homo sapiens 
2014-07-12 | E-GEOD-59335 | biostudies-arrayexpress
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