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ProteomicsA CHD8-TRRAP axis facilitates MYC- and E2F-target gene regulation in human neural stem cells.
Mutations in ATP-dependent chromatin remodeler CHD8 cause one of the most frequent monogenetic forms of autism and also associate with brain overgrowth. Nevertheless, activities of CHD8 in autism-relevant cell types are still poorly understood. Here we purify the CHD8 protein from human neural stem ...
ORGANISM(S): Homo sapiens (Human) 
2025-05-06 | PXD044582 | Pride
Neural stem cells Chd8 Myc Gene regulation Ez2f 
TranscriptomicsGenome-wide analysis of CHD8 binding sites
Genome-wide analysis of CHD8 binding sites as well as sites associated with the activating histone marks 2mk4H3 and 3mK4H3. These analyses have been performed by ChIP-chip using a custom microarray containing promoter regions and CpG islands. ChIP experiments were performed with the C33A human cervi...
ORGANISM(S): Homo sapiens 
2013-12-03 | E-GEOD-51564 | biostudies-arrayexpress
TranscriptomicsTranscriptome of iPSC-derived Neural Cells with Heterozygous Knockout in CHD8
CHD8, encoding Chromodomain helicase DNA binding protein 8, is a top autism spectrum disorders (ASDs) risk gene. To better understanding the molecular links between CHD8 functions and ASD, we have applied the CRISPR/Cas9 technology to knockout one copy of CHD8 in induced pluripotent stem cells (iPSC...
ORGANISM(S): Homo sapiens 
2015-11-05 | E-GEOD-71594 | biostudies-arrayexpress
ProteomicsCHD8 Suppression Impacts on Histone H3 Lysine 36 Trimethylation and Alters RNA Alternative Splicing
Disruptive mutations in the chromodomain helicase DNA binding protein 8 (CHD8) have been recurrently associated with Autism Spectrum Disorders (ASD). Here we investigated how chromatin reacts to CHD8 suppression by analyzing a panel of histone modifications in induced pluripotent stem cell-derived n...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD025739 | Pride
Autism spectrum disorder Chd8 Alternative splicing Chromatin H3k36me3 
GenomicsRNAseq data from ES and differentiated cells in Chd8 WT vs Chd8 KD and Chd8 KO cells
RNAseq data from ES and differentiated cells in Chd8 WT vs Chd8 KD and Chd8 KO cells
PRJNA702132 | ENA
ProteomicsRole of Chd8 in Xist regulation
In this project, we have studied the role of Chd8 in Xist regulation and XCI initiation by means of Chd8 Knock-Downs (KD) and Knock-Out (KO).
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2021-09-10 | PXD024155 | Pride
Epigenetics X chromosome inactivation (xci) Xist 
ProteomicsSINEUP RNA rescues molecular phenotypes associated with CHD8 suppression in Autism Spectrum Disorder model systems
Loss-of-function mutations in the chromodomain helicase DNA-binding 8 (CHD8) gene are strongly associated with Autism Spectrum Disorders (ASD). Indeed, the reduction of CHD8 causes transcriptional, epigenetic and cellular phenotypic changes, correlated to disease that can be monitored in assessing n...
ORGANISM(S): Homo sapiens (Human) 
2025-02-24 | PXD056482 | Pride
Chd8  sineup  autism spectrum disorders (asd)  lncrna  neurodeveloment  zebrafish 
TranscriptomicsThe chromatin modifier CHD8 targets autism risk genes during human neurodevelopmentM-BM-
Whole-exome sequencing studies have implicated chromatin modifiers and transcriptional regulators in autism spectrum disorder (ASD) through the identification of de novo loss of function mutations in affected individuals. Many of these genes are co-expressed in mid-fetal human cortex, suggesting ASD...
ORGANISM(S): Mus musculus 
2015-01-21 | E-GEOD-57369 | biostudies-arrayexpress
GenomicsTranscriptome analysis of CHD8 ko mice
Transcriptome analysis of CHD8 ko mice
PRJNA757969 | ENA
TranscriptomicsBulk RNA-seq of cerebral organoids investigating the role of CHD8 in cortical development
We used cerebral organoids generated from wildtype and CHD8 +/- human ES cells to study the effects of CHD8, one of the top ASD risk genes, on early cortical development. CHD8 +/- hESC were generated using the CRISPR/Cas9 system to create a deletion within the helicase domain. Cerebral organoids wer...
ORGANISM(S): Homo sapiens 
2022-02-01 | E-MTAB-9933 | biostudies-arrayexpress
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