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ProteomicsStructural- and Site-specific N-glycosylation Characterization of COVID-19 Virus Spike with StrucGP
Spike (S) protein plays a key role in COVID-19 (SARS-CoV-2) infection and host-cell entry. Previous studies have systematically analyzed site-specific glycans compositions of S protein. Here, we further provide structure-clear N-glycosylation of S protein at site-specific level by using our recently...
ORGANISM(S): Homo sapiens (Human) 
2022-10-13 | PXD034086 | Pride
Covid-19 Lc-msms Strucgp Spike Glycopeptides 
ProteomicsQuantitative proteomic analysis of four subtypes of M2 macrophages
In this study, we used mass spectrometry and label-free quantification (LFQ) to characterize the global proteomics of polarized (M1, M2a, M2b, M2c, and M2d) and unpolarized (M0) phenotypes of macrophages from human THP-1 monocytes. The results described the biological functions of the four M2 macrop...
ORGANISM(S): Homo sapiens (Human) 
2022-08-12 | PXD022320 | Pride
M2 macrophage Prm Polarization Biomarker Lc-ms/ms 
ProteomicsN-GlycositeAtlas, a database resource for mass spectrometry-based human N-linked glycoproteins and glycosylation sites
N-Linked glycoprotein is a highly interesting class of proteins for clinical and biological research. The large-scale characterization of N-linked glycoproteins accomplished by mass spectrometry-based glycoproteomics has provided valuable insights into the interdependence of glycoprotein structure a...
ORGANISM(S): Homo sapiens (Human) 
2019-11-08 | PXD005143 | Pride
Mass spectrometry Glycoprotein Protein glycosylation Database Glycoproteome 
ProteomicsQuantitative glycoproteomic, proteomic, and phosphoproteomic analysis of aged mouse ovary
In this work, we investigate the differences of site-specific N-glycosylation between young (2-month-old) and middle-aged (12-month-old) mouse ovarian tissues by integrating N-glycoproteomics and proteomic approaches with tandem mass tag (TMT) labeling.To identify signalling pathways associated with...
ORGANISM(S): Mus Musculus 
2024-09-14 | PXD056126 |
ProteomicsProfiling of human spermatozoa glycoproteins by LC/MS/MS
N-linked glycoproteins are known to be important for spermatozoa development and gametes fusion. However, little is known about detailed information of these glycoproteins, particularly the glycosylation sites and its corresponding glycan structures in human spermatozoa. In present study, an in hous...
ORGANISM(S): Homo sapiens (Human) 
2023-03-04 | PXD020286 | Pride
Human Lc/ms/ms Glycan structures Spermatozoa 
ProteomicsGlycoproteomic analysis of Fut8 knock out/wild type mouse brain, Human IgG and Human Serum.
In this study, we provide a simple and straightforward method to distinguish core-fucosylated glycans from antenna-fucosylated glycans by systematically evaluating the extent of fucose migration based on the feature Y ions by using Fut8 knockout (Fut8−/−) mouse brain under HCD energy of 20%. The me...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2022-11-14 | PXD035158 | Pride
Fut8 Glycoproteomics Human igg Human serum Mouse brain Lc-ms/ms 
ProteomicsEvaluation of absorbent cotton for glycopeptide enrichment
This study introduced the use of cotton in 200µL tips, namely cotton HILIC as a convenient tool for enriching large-scale glycopeptides before MS analysis in this study. The optimal loading buffer for cotton HILIC was investigated by using mouse brain as a complex test sample. Subsequently, the perf...
ORGANISM(S): Mus musculus (Mouse) 
2023-03-04 | PXD035078 | Pride
Hilic Glycopeptide Enrichment absorbent cotton Mass spectrometry Max 
ProteomicsPrecision structural interpretation of site-specific N-glycans in seminal plasma
This study is aimed to comprehensively and globally characterize seminal plasma glycoproteins in a site-specific manner with information of N-glycosites and corresponding glycan structures, as well as glycan isomers using mass spectrometry. Furthermore, the function of specific glycan structures is ...
ORGANISM(S): Homo sapiens (Human) 
2023-03-04 | PXD030804 | Pride
Seminal plasma Glycoproteomics Glycan structures Mass spectrometry Intact glycopeptides 
ProteomicsPrecision mapping of glycosite-specific glycans reveals distinctive N-glycosylation on human spermatozoa
In this study, we applied the recently developed software, StrucGP to large-scale characterize glycoproteins, and IGPs, as well as precise site-specific glycan structures in human spermatozoa. Furthermore, bioinformatical analyses were conducted to systematically describe the potential functions of ...
ORGANISM(S): Homo sapiens (Human) 
2022-05-19 | PXD026649 | Pride
Spermatozoa Glycan structures Mass spectrometry 
ProteomicsPrecision Characterization of Site-specific O-Acetylated Sialic Acids on N-Glycoproteins
O-Acetylation is a common modification of sialic acid, playing a significant role in glycoprotein stability, immune response, and cell development. The lack of efficient methods for direct analysis of O-acetylated sialoglycopeptides (O-AcSGPs) made the majority of identified O-acetylated sialic acid...
ORGANISM(S): Mus musculus (Mouse) 
2023-01-13 | PXD037161 | Pride
Glycoproteomics Strucgp Mouse serum O-acetylated sialic acids 
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