Sort   by:  
 Page size 
ProteomicsSingle cell analysis for oocytes and early embryos
While non-mammalian embryos often rely on spatial pre-patterning, for decades it was believed that blastomeres of mouse and human embryos were developmentally equivalent. However, emerging evidence suggests early mouse and human blastomeres differ in developmental potential. Here, using multiplexed ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Mus Musculus (ncbitaxon:10090) 
2025-08-10 | MSV000098778 | MassIVE
ProteomicsIn-Depth Comparison of Reagent-Based Digestion Methods and Two Commercially Available Kits for Bottom-Up Proteomics
We assessed two physical disruption methods to homogenize cells —sonication and BeatBox—alongside four digestion protocols. Two of them are lab-reagent strategies: urea-based, and SDC (Sodium deoxycholate)-based in-solution digestion, and two are commercially available kits: the EasyPep kit from The...
ORGANISM(S): Homo sapiens (Human) 
2026-01-07 | PXD056409 | Pride
In-solution digestion Lc-ms Proteomics Proteolysis Bottom-up proteomics 
ProteomicsTemporal proteomics of p97/VCP inhibition
To facilitate the development of p97 inhibitors as potential therapeutic agents and help define their clinical application, it is necessary to dissect the mechanism of action (MOA) of p97 inhibitors and compare them with proteasome inhibitors. Moreover, identifying specific cellular markers is criti...
ORGANISM(S): Homo sapiens (Human) 
2021-12-15 | PXD025225 | Pride
Proteasome inhibitor Hct116 P97 inhibitor Proteomic 
ProteomicsTemporal proteomics of p97/VCP inhibition
To facilitate the development of p97 inhibitors as potential therapeutic agents and help define their clinical application, it is necessary to dissect the mechanism of action (MOA) of p97 inhibitors and compare them with proteasome inhibitors. Moreover, identifying specific cellular markers is criti...
ORGANISM(S): Homo sapiens (Human) 
2021-12-15 | PXD025167 | Pride
Proteasome inhibitor Hct116 P97 inhibitor Proteomic 
ProteomicsTemporal proteomics of p97/VCP inhibition
To facilitate the development of p97 inhibitors as potential therapeutic agents and help define their clinical application, it is necessary to dissect the mechanism of action (MOA) of p97 inhibitors and compare them with proteasome inhibitors. Moreover, identifying specific cellular markers is criti...
ORGANISM(S): Homo sapiens (Human) 
2021-12-15 | PXD025094 | Pride
Proteasome inhibitor Hct116 P97 inhibitor Proteomic 
ProteomicsAmino acid analog induces stress response in marine Synechococcus
In this study, we explored the use of BONCAT in Synechococcus sp. – a globally important cyanobacteria. We characterized the growth and microscopically quantified HPG uptake under a range of HPG concentrations in marine Synechococcus sp. Further, we examined changes in protein expression of Synechoc...
ORGANISM(S): Synechococcus sp. CC9311 
2021-04-01 | PXD023371 | Pride
Boncat Phytoplankton Synechococcus Ncaas 
ProteomicsThe Phenyl Indole p97 inhibitor, UPCDC-30245, blocks endo-lysosomal degradation and coronavirus entry to host cells
Different modes of action small molecule inhibitors provide versatile tools for investigation of biology and development of therapeutics. However, to evaluate their exact mechanism of actions remains to be a challenging tas. We have identified two classes of p97 inhibitors, ATP competitive and allos...
ORGANISM(S): Homo sapiens (Human) 
2022-04-04 | PXD025822 | Pride
P97 inhibitor  proteomic  anti-virus 
ProteomicsMechanisms of chlorate toxicity and resistance in Pseudomonas aeruginosa
Pseudomonas aeruginosa is an opportunistic pathogen known to cause both acute and chronic infections. P. aeruginosa often encounters hypoxic/anoxic environments within the host, which increases its tolerance to many conventional antibiotics. Towards identifying novel therapeutic treatments, we have ...
ORGANISM(S): Pseudomonas aeruginosa (strain UCBPP-PA14) 
2023-10-24 | PXD033396 | Pride
Resistance Chlorate toxicity Pseudomonas aeruginosa 
ProteomicsRegulation of mitophagy by Fis1 and Fascin1-organized actin
Mitophagy, the autophagic degradation of mitochondria, plays a central role in controlling the quality and quantity of mitochondria, thereby ensuring cellular health. The mitochondrial outer membrane protein Fis1 is important for several types of mitophagy, but its mechanism of action remains unclea...
ORGANISM(S): Mus musculus (Mouse) 
2026-07-07 | PXD062948 | Pride
Mitophagy Fascin1 Fis1 Mitochondria Actin filaments 
ProteomicsNMS-873 leads to dysfunctional glycometabolism in a p97-independent manner
ATP-competitive p97 inhibitor CB-5339, the successor of CB-5083, is being evaluated in Phase 1 clinical trials for anti-cancer therapy. Different modes of action p97 inhibitors such as allosteric inhibitors are useful to overcome one the major problems of targeted therapy: drug-induced resistance. W...
ORGANISM(S): Homo sapiens (Human) 
2022-05-19 | PXD025898 | Pride
Protein stability Proteomics Resistance P97 inhibitor Glycometabolism 
Sort   by:  
 Page size