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Profiling the chromatin-bound proteome (chromatome) in a simple, direct, and reliable manner might be key to uncovering the role of yet uncharacterized chromatin factors in physiology and disease. Here, we have design an experimental strategy to survey the chromatome of proliferating cells by using ...
ORGANISM(S): Mus musculus (Mouse) 
2019-03-13 | PXD011670 | Pride
Chromatin-associated and RNA-dependent Chromatin-associated proteome from S. cerevisiae, obtained by a new method yChEFs(yeast Chromatin Enriched Fractions)
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2019-07-17 | PXD009264 | Pride
Linking chromatin acylation mark-defined proteome and genome in living cells
Linking chromatin acylation mark-defined proteome and genome in living cells
Linking chromatin acylation mark-defined proteome and genome in living cells
Linking chromatin acylation mark-defined proteome and genome in living cells
Linking chromatin acylation mark-defined proteome and genome in living cells
In this project, 3XFlag-tagged protein affinity purifications were performed to identify the protein-protein interactors using Cryptococcus neoformans cell lysate. After fractionation with SDS-PAGE, peptide samples for nanoLC-MS/MS were prepared by in-gel digestion. Raw data from Orbotrap Velos pr...
ORGANISM(S): Cryptococcus Neoformans 
The aim of the project was to analyse the role of DONSON during DNA replication reaction in Xenopus laevis egg extract. To test what is the impact of DONSON on replication we decided to remove DONSON from egg extract by immunodepletion and analyse its impact on DNA replication reaction. In the paral...
ORGANISM(S): Xenopus laevis (African clawed frog) 
2023-10-24 | PXD042435 | Pride
Chromatin processes such as DNA replication, transcription and RNA processing are mediated by intermolecular transactions among proteins, RNAs and the genome. Although DNA-protein and RNA-protein interactions have been studied extensively, reliable and efficient identification of chromatin-associate...
ORGANISM(S): Mus musculus (Mouse) 
2021-11-25 | PXD023903 | Pride
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