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ProteomicsReprogramming the Lipid Peroxidation Product 4-ONE as a Chemoselective Cleavable Crosslinker
Nature builds and stabilizes proteins through covalent biological glues, yet the logic and traceability of metabolite-driven crosslinking remain obscure. Here we reprogram the lipid peroxidation product 4-oxo-nonenal (4-ONE) into a metabolite-derived Cys-Lys biological glue and mass-cleavable crossl...
ORGANISM(S): Homo sapiens (Human) 
2026-06-22 | PXD077887 | Pride
4-one Peroxidation Mass spectrometry Crosslink Lipid 
ProteomicsReprogramming the Lipid Peroxidation Product 4-ONE as a Chemoselective Cleavable Crosslinker
Nature builds and stabilizes proteins through covalent biological glues, yet the logic and traceability of metabolite-driven crosslinking remain obscure. Here we reprogram the lipid peroxidation product 4-oxo-nonenal (4-ONE) into a metabolite-derived Cys-Lys biological glue and mass-cleavable crossl...
ORGANISM(S): Homo sapiens (Human) 
2026-06-22 | PXD077820 | Pride
4-one Peroxidation Mass spectrometry Crosslink Lipid 
ProteomicsImproved peptide backbone fragmentation is the primary advantage of MS-cleavable crosslinkers
Proteome-wide crosslinking mass spectrometry studies have coincided with the advent of MS-cleavable crosslinkers that can reveal the individual masses of the two crosslinked peptides. However, recently such studies have also been published with non-cleavable crosslinkers suggesting that MS-cleavabil...
ORGANISM(S): Mus musculus (Mouse) Escherichia coli Drosophila melanogaster (Fruit fly) 
2022-06-24 | PXD032821 | Pride
Lc-msms Dsbu Ms-cleavable crosslinker Dsso Crosslinking Clms 
ProteomicsLight-induced orthogonal fragmentation of crosslinked peptides
Crosslinking mass spectrometry provides pivotal information on the structure and interaction of proteins. MS-cleavable crosslinkers are regarded as a cornerstone for the analysis of complex mixtures. Yet they fragment under similar conditions as peptides, leading to mixed fragmentation spectra of th...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2023-08-28 | PXD040267 | Pride
Clms Cleavable crosslinker Crosslinking Uvpd 
ProteomicsImproving identification of in-organello protein-protein interactions using an affinity-enrichable, isotopically-coded, and mass spectrometry-cleavable chemical crosslinker
An experimental and computational approach for identification of protein-protein interactions by in-vivo chemical crosslinking and mass spectrometry (CLMS) has been developed that takes advantage of the specific characteristics of cyanurbiotindipropionylsuccinimide (CBDPS), an affinity-tagged isotop...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-03-17 | PXD017066 | Pride
Cross-linking Yeast Mitochondria 
ProteomicsImproving identification of in-organello protein-protein interactions using an affinity-enrichable, isotopically-coded, and mass spectrometry-cleavable chemical crosslinker
An experimental and computational approach for identification of protein-protein interactions by in-vivo chemical crosslinking and mass spectrometry (CLMS) has been developed that takes advantage of the specific characteristics of cyanurbiotindipropionylsuccinimide (CBDPS), an affinity-tagged isotop...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-03-17 | PXD014055 | Pride
Cross-linking Yeast Mitochondria 
ProteomicsIdentification of MS-Cleavable and Non-Cleavable Chemically Crosslinked Peptides with MetaMorpheus
The present work describes a novel search program for the detection of both MS-cleavable and non-cleavable crosslinked peptides and it is the first software program reported with both capacities. The search strategy has been implemented in the computer program MetaMorpheus, which has a user-friendly...
ORGANISM(S): Escherichia coli 
2018-07-16 | PXD009000 | Pride
Proteomics Ms-cleavable chemical crosslink Protein-protein interactions Ion index 
UnknownDeveloping a new cleavable crosslinker reagent for in-cell crosslinking.
Not available
S-EPMC12185727 | biostudies-literature
ProteomicsEvaluation of cleavable crosslinking for characterization of proteoform structural differences by top-down mass spectrometry
Cleavable crosslinking has traditionally been employed in bottom-up mass spectrometry to elucidate protein structure and protein-protein interactions through identification of peptides bearing characteristic mass adducts. Here, we demonstrate the application of cleavable crosslinking in top-down mas...
ORGANISM(S): Homo Sapiens (human) Equus Caballus 
2026-01-17 | PXD073175 | JPOST Repository
ProteomicsCleavable Photo-Crosslinker Enables sFFP-Driven Strategy for Mapping HSP90β Oligomer Structure
Crosslinking technology is a pivotal tool for resolving dynamic protein interaction networks. While traditional chemical crosslinkers face limitations in sensitivity, reaction kinetics, and in situ activation, cleavable and photo-crosslinkers have emerged as alternatives. However, their complex spec...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2026-04-09 | PXD064219 | Pride
Photo-cross-link Hsp90 Randomforest Cleavable cross-linker 
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