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ProteomicsEnzyme and Chemical Assisted N-Terminal Blocked Peptides Analysis, ENCHANT, as a Selective Proteomics Approach Complementary to Conventional Shotgun Approach
Shotgun (bottom-up) approach has been widely applied in large-scale proteomics studies. The inherent shortages of shotgun approach lie in that the generated peptides often overwhelm the analytical capacity of current LC-MS/MS systems and that abundant proteins often hamper the identification of prot...
ORGANISM(S): Homo sapiens (Human) 
2017-11-30 | PXD007863 | Pride
Dia Single-charge ion inclusion N-terminal blocked peptides Cnbr-activated sepharose 4b 
UnknownCloning, expression and purification of the factor H binding protein and its interaction with factor H.
Not available
S-EPMC4833738 | biostudies-literature
UnknownPreparation and characterization of polyclonal antibodies against ARL-1 protein.
Not available
S-EPMC4615481 | biostudies-literature
UnknownAffinity Isolation and Mass Spectrometry Identification of Prostacyclin Synthase (PTGIS) Subinteractome.
Not available
S-EPMC6628129 | biostudies-literature
ProteomicsIP-MS target protein identification
To identify the target of the monoclonal antibody, Immuoprecipitation (IP) was performed to pull-down target protein from cell culture extraction, corn or zebrafish tissue lysate. The IP product was validated on silver-stained SDS-PAGE and Western blot. The target band was cut out and performed LC-M...
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) Homo sapiens (Human) Zea mays (Maize) 
2020-05-26 | PXD011629 | Pride
Human Maize Zebrafish Protein identification 
ProteomicsSubstantial Influence of ERAP2 on the HLA-B*40:02 peptidome. Implications for HLA-B*27-negative ankylosing spondylitis.
HLA-B*40:02 is one of a few Major Histocompatibility Complex class I (MHC-I) molecules associated with ankylosing spondylitis (AS) independently of HLA-B*27. The endoplasmic reticulum aminopeptidase 2 (ERAP2), an enzyme that process MHC-I ligands and preferentially trims N-terminal basic residues, i...
ORGANISM(S): Homo sapiens (Human) 
2019-09-25 | PXD014804 | Pride
Hla-b*40:02 peptidome Hla-b*27 Ankylosing spondylitis Erap2 
ProteomicsThe Behçet’s Disease associated Hap10 variant of ERAP1 generates a low affinity HLA-B*51 peptidome through differential processing of two subpeptidomes
HLA-B*51, the main risk factor for Behçet’s disease (BD), binds two main subpeptidomes consisting of low affinity peptides with Ala as their second amino acid and higher affinity peptides with Pro at this position. A low activity variant of ERAP1, Hap10, is uniquely associated with BD susceptibility...
ORGANISM(S): Homo sapiens (Human) 
2018-10-24 | PXD006215 | Pride
Behçet's disease Erap1 Hla-b*51 Antigen processing 
ProteomicsInfection of RAW 264.7 cells by Murine Leukemia Virus-derived retroviral pseudotype expressing the envelope glycoprotein of EBOV is enhanced by immune serum and monoclonal antibodies
It has been shown that antibodies can enhance Ebola virus (EBOV) infection at sub-neutralizing concentrations. The effect of antibodies on the infection of murine cell line (RAW 264.7) with MLV-EBOV, a murine leukemia virus pseudotype expressing surface glycoprotein (GP) of EBOV and green fluorescen...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2026-01-26 | PXD066513 | Pride
Virus Monoclonal antibodies Proteomic Ebola Epitope 
ProteomicsRedundancy and Complementarity between ERAP1 and ERAP2 Revealed by their Effects on the Behçet’s Disease-Associated HLA-B*51 Peptidome
The endoplasmic reticulum aminopeptidases ERAP1 and ERAP2 trim peptides to be loaded onto HLA molecules, including the main risk factor for Behçet’s disease HLA-B*51. ERAP1 is also a risk factor among HLA-B*51-positive individuals, whereas no association is known with ERAP2. This study addressed the...
ORGANISM(S): Homo sapiens (Human) 
2019-05-20 | PXD012348 | Pride
Hla-b*51 Erap1 Behçet’s disease Erap2 Peptidome 
ProteomicsB27 inmunopeptidome in vaccinica infected cells
Identification of natural human leukocyte antigen (HLA) ligandome is a key element to understand the cellular immune responses. Advanced high throughput mass spectrometry analyses identify a relevant, but not complete, fraction of the many tens of thousands of self-peptides generated by the antigen ...
ORGANISM(S): Homo sapiens (Human) 
2021-12-22 | PXD027859 | Pride
Hla-b*27 Vaccinica Hla Immunopeptidomics 
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