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ProteomicsA gephyrin-like protein links cell division and polar elongation in Corynebacteriales
To discover missing players in corynebacterial cell division we used mass-spectrometry based interactomics using formaldehyde as crosslinker, starting with the FtsZ membrane anchor SepF as a bait for co-immunoprecipitation (co-IP) of divisome members. For SepF interactome we carried out the co-IPs u...
ORGANISM(S): Corynebacterium glutamicum ATCC 13032 
2023-07-26 | PXD037255 | Pride
Cell division Corynebacterium glutamicum Interactomics 
GenomicsAberrant neurodevelopment in human iPS cell-derived astrocyte-neuron co-culture model of Alexander disease
Aberrant neurodevelopment in human iPS cell-derived astrocyte-neuron co-culture model of Alexander disease
PRJNA1085307 | ENA
TranscriptomicsAberrant neurodevelopment in human iPS cell-derived astrocyte-neuron co-culture model of Alexander disease
Co-cultures of neurons and astrocytes derived from human iPS cells carrying a GFAP (R239C) mutation - an identified cause of Alexander disease (AxD) - were analyzed with single-cell RNA sequencing to assess cell population composition, differential gene expression, and cell-cell interaction differen...
ORGANISM(S): Homo sapiens 
2024-04-07 | GSE261110 | GEO
ProteomicsSalt stress phosphorylation network
We utilized high resolution, high mass accuracy quantitative proteomics to explore stress signaling in yeast. We accessed changes in protein phosphorylation at various time points after exposure to salt stress and used this information to reconstruct stress signaling networks. We performed similar e...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2018-04-05 | PXD006192 | Pride
Phosphorylation Yeast Environmental stress response Stress signaling 
TranscriptomicsInduced Pluripotency of Human Prostatic Epithelial Cells
Induced pluripotent stem (iPS) cells are a valuable resource for discovery of epigenetic changes critical to cell type-specific differentiation. Although iPS cells have been generated from other terminally differentiated cells, the reprogramming of normal adult human basal prostatic epithelial (E-PZ...
ORGANISM(S): Homo sapiens 
2016-07-03 | E-GEOD-45469 | biostudies-arrayexpress
UnknownComprehensive analysis of metabolites produced by co-cultivation of Bifidobacterium breve MCC1274 with human iPS-derived intestinal epithelial cells
Not available
S-EPMC10133457 | biostudies-literature
TranscriptomicsRole of the reprogramming factors in inducing pluripotency
Induced pluripotent stem (iPS) cells can be obtained from fibroblasts by expression of Oct4, Sox2, Klf4, and c-Myc. To determine how these factors induce this change in cell identity, we carried out genomewide promoter analysis of their binding in iPS and partially reprogrammed cells. Most targets i...
ORGANISM(S): Mus musculus 
2010-05-06 | E-GEOD-14012 | biostudies-arrayexpress
ProteomicsPLZF and DDX5 interacting proteins in spermatogonia
DDX5, or PLZF co-immunoprecipitation in lysates from cultured undifferentiated spermatogonia followed by identification of eluted proteins using mass spectrometry. IgG control IPs included.
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2019-02-24 | MSV000083476 | MassIVE
ProteomicsIdentification of proteins associated with the decapping complex and the endonuclease DNE1 in Arabidopsis
In this work, we used an unbiased approach coupling immunoprecipitations (IPs) and mass spectrometry to define the interactome of the decapping activator DCP1 and the decapping enzyme DCP2. In addition we unravel the interactome of the endonuclease DNE1 harboring an endoribonuclease domain of the NY...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2021-11-16 | PXD020780 | Pride
Dcp1 Mrna decapping Dne1 Rna degradation P-bodies Proteome Nyn domain Mass spectrometry 
ProteomicsReprogramming of flagellin receptor responses with surrogate ligands
A proteomic analysis using co-immunoprecipitations (Co-IPs) coupled to mass spectrometry to compare the FLS2 interactomes induced by the natural ligand or the surrogates. proFLS2:FLS2-GFP line was used for treatment either DMSO, Maya1, Maya2, or flg22 and wild-type (Col 0) was used for negative cont...
ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress) 
2024-09-27 | PXD056313 | JPOST Repository
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