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MetabolomicsProteomic analysis of osteocyte line (MLO-Y4) with knockout of cystic fibrosis transmembrane conductance regulator (CFTR) or chloride deprivation treatment

Mass spectrometry–based proteomic analysis of an osteocyte line, MLO-Y4, was conducted to profile global protein expression changes under two experimental settings, 1) CRISPR-Cas9-based knockout of CFTR versus wild-type; 2) Cl- deprivation versus normal extracellular medium.

2026-03-27 | MTBLS14165 | MetaboLights
ProteomicsCRISPR-Cas9-based knockout of the prion protein and its effect on the proteome
The molecular function of the cellular prion protein (PrPC) and the mechanism by which it may contribute to neurotoxicity in prion diseases and Alzheimer’s disease (AD) are only partially understood. Mouse neuroblastoma Neuro2a cells and, more recently, C2C12 myocytes and myotubes have emerged as po...
ORGANISM(S): Mus musculus (Mouse) 
2014-11-19 | PXD001301 | Pride
Crispr-cas9 Knockout Prion Kockdown 
TranscriptomicsGenome-wide CRISPR-Cas9 screens reveal loss of redundancy between PKMYT1 and WEE1 in Glioblastoma stem-like cells
To identify new therapeutic targets for Glioblastoma (GBM), we performed genome-wide CRISPR-Cas9 "knockout" (KO) screens in patient-derived GBM stem-like cells (GSCs) and human neural stem/progenitors (NSCs), non-neoplastic stem cell controls, for genes required for their in vitro growth. ...
ORGANISM(S): Homo sapiens 
2016-02-15 | E-GEOD-70038 | biostudies-arrayexpress
MetabolomicsMetabolomic Profiling of the Striatum in Shank3 Knockout ASD Rats: Effects of Early Swimming Regulation

OBJECTIVE: This study aimed to investigate the regulatory impact of early swimming intervention on striatal metabolism in Shank3 gene knockout ASD model rats.

METHODS: Shank3 gene knockout exon 11-21 male 8-day-old SD rats were used as experimental subject...

2024-09-27 | MTBLS8577 | MetaboLights
ProteomicsChanges of Protein Expression after CRISPR/Cas9 Knockout of miRNA-142 in Cell Lines Derived from Diffuse large B-Cell Lymphoma // Proteome Dataset
MicroRNAs (miRNAs) post-transcriptionally regulate gene expression by inhibiting protein synthesis of target messenger RNAs (mRNAs). MicroRNA-142 (miR-142), which has tumor-suppressive properties, was functionally deleted by CRISPR/Cas9 knockout in cell lines derived from diffuse large B-cell lympho...
ORGANISM(S): Homo sapiens (Human) 
2023-03-10 | PXD037148 | Pride
Microrna-142  crispr/cas9  diffuse large b-cell lymphoma  mir-142 knockout cell lines  proteomics  transcriptomics  akt1s1  ccnb1  lima1  tfrc 
TranscriptomicsTranscriptome of iPSC-derived Neural Cells with Heterozygous Knockout in CHD8
CHD8, encoding Chromodomain helicase DNA binding protein 8, is a top autism spectrum disorders (ASDs) risk gene. To better understanding the molecular links between CHD8 functions and ASD, we have applied the CRISPR/Cas9 technology to knockout one copy of CHD8 in induced pluripotent stem cells (iPSC...
ORGANISM(S): Homo sapiens 
2015-11-05 | E-GEOD-71594 | biostudies-arrayexpress
ProteomicsCRISPR/Cas9-mediated knockout and endogenous complex analysis of Cluap1/ IFT38
CRISPR/Cas9-mediated gene-editing allows manipulation of a gene of interest in its own chromosomal context. When applied to the analysis of protein interaction, and in contrast to an exogenous expression of a protein, these can be studied maintaining physiological stochiometries, topology and contex...
ORGANISM(S): Homo sapiens (Human) 
2018-04-04 | PXD008343 | Pride
Cilia Proteomics Crispr/cas9 Ift 
ProteomicsWhole cell proteomics analysis of Trypanosoma brucei 75kDa invariant surface glycoprotein knockout cells
A superfamily of invariant surface glycoproteins (ISGs) populates the African trypanosome surface, one of which, ISG75, is implicated in uptake of the century-old drug suramin. We disrupted the ISG75 gene locus encoding an array of six ISG75 paralogs on chromosome V by CRISPR/Cas9 knockout. Here we ...
ORGANISM(S): Trypanosoma brucei 
2023-03-11 | PXD031887 | Pride
Xenobiotics Drug metabolism Drug accumulation Trypanosome Crispr/cas9 Invariant surface glycoprotein Suramin 
GenomicsIn vivo Genome-scale CRISPR-Cas9 knockout screening
In vivo Genome-scale CRISPR-Cas9 knockout screening
PRJNA515515 | ENA
GenomicsGenome-wide CRISPR-Cas9 knockout screen in MCF10A with ATP-competitive inhibition of CDC7 (XL-413)
The coordinated activation of DNA replication origins is important for efficient DNA synthesis and genome stability. S-phase cyclin dependent kinases (CDKs) together with CDC7 kinase, are essential to origin activation by converting the pre-replicative complex into a fully active helicase. To identi...
ORGANISM(S): Homo sapiens 
2026-09-30 | E-MTAB-17434 | biostudies-arrayexpress
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