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ProteomicsHEK293T cell nuclear & cytosolic proteome remodelling upon amino acid withdrawal. Related to Supplementary Table 4 from An et al (2020)
HEK293T proteome cytosolic vs nuclear fractionation upon short (3h) amino acids withdrawal.
ORGANISM(S): Homo sapiens (Human) 
2020-04-30 | PXD017856 | Pride
Human Lc-msms Cytosolic nuclear fractionation 
ProteomicsHEK293T cell nuclear & cytosolic phospho-proteome remodelling upon amino acid withdrawal. Related to Supplementary Table 4 from An et al (2020)
HEK293T Phospho proteome cytosolic vs nuclear fractionation upon short (3h) amino acids withdrawal.
ORGANISM(S): Homo sapiens (Human) 
2020-04-30 | PXD017855 | Pride
Human Lc-msms Cytosolic nuclear fractionation Phospho 
TranscriptomicsHeLa cell mitochondrial microRNAs
MicroRNAs (miRNAs) are small non-coding RNAs that associate with Argonaute 2 protein to regulate gene expression at the post-transcriptional level in the cytoplasm. However, recent studies have reported that some miRNAs localize to and function in other cellular compartments. Mitochondria harbour th...
ORGANISM(S): Homo sapiens 
2011-10-05 | E-GEOD-24761 | biostudies-arrayexpress
TranscriptomicsEfficient cellular fractionation of RNA
The aim of this experiment was to develop new methods to extract pure fractions of nuclear and cytoplasmic RNA. We compared the results of nuclear and cytoplasmic RNA-sequencing to results of the total and polyA+ RNA-sequencing. Cytoplasmic, nuclear, total and polyA+ RNA was extracted from two human...
ORGANISM(S): Homo sapiens 
2013-10-22 | E-MTAB-1898 | biostudies-arrayexpress
TranscriptomicsRNA-sequencing in SH-SY5Y neuroblastoma cell line with TDP-43 KD and nuclear and cytoplasmic fractionation as well and treatment with the nonsense-mediated decay inhibition using SMG1-11j inhibitor
TDP-43 knockdown produces aberrant NMD-sensitive cryptic exons. To discover where TDP-43 cryptic exons were localized and which were sensitive to NMD-inhibition, we performed cellular fractionation as well and NMD-inhibition with TDP-43 KD
ORGANISM(S): Homo sapiens 
2026-07-04 | E-MTAB-15653 | biostudies-arrayexpress
ProteomicsTargeted quantitative analysis of specific proteins in cytosolic, mitochondrial, and nuclear fractions using PRM
We present a targeted proteomic approach using parallel reaction monitoring (PRM) to assess the enrichment of cytosolic, mitochondrial, and nuclear fractions obtained from liver samples using commercial isolation kits. For mitochondrial fraction, CPS1 and ATP5F1 were chosen. For the cytosolic fracti...
ORGANISM(S): Homo sapiens (Human) 
2026-04-13 | PXD067737 | Pride
Mitochondrial enrichment Liver Prm Subcellular fractionation Target proteomic 
ProteomicsNuclear Pyruvate Dehydrogenase Complex regulates EIN2-directed Histone Acetylation and Transcriptional Regulation in the Ethylene Response.
Histone acetylation is important for the transcriptional regulation in the ethylene response and the nuclear metabolic production of acetyl coenzyme A (acetyl-CoA) plays key roles in histone acetylation. But how the nucleus produces acetyl-CoA for histone acetylation and transcription regulation in ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-06-25 | PXD040689 | Pride
Pyruvate dehydrogenase complex Protein phosphorylation 
ProteomicsNuclear TRiC states captured in situ
The chaperonin T-complex protein ring complex (TRiC) is an essential protein-folding machine in eukaryotic cells. Although its cytosolic role in folding newly synthesized proteins is well established, nuclear TRiC remains less well described. Here, we combined cryo-electron tomography (cryo-ET), cro...
ORGANISM(S): Homo sapiens (Human) 
2026-09-09 | PXD083885 | Pride
Ap-ms Cryo-et Tric complex 
UnknownDouble-Strand Breaks Induce Nuclear-Cytosolic Shuttling of Polymorphic DNA Mismatch Repair Protein MutS Homolog 3 and Binding to NEMO/IKKγ in Colon Cancer Cells.
Not available
S-EPMC12450631 | biostudies-literature
TranscriptomicsGenome-Scale Identification of Membrane-Associated Human mRNAs
The subcellular localization of proteins is critical to their biological roles. Moreover, whether a protein is membrane-bound, secreted, or intracellular affects the usefulness of, and the strategies for, using a protein as a diagnostic marker or a target for therapy. We employed a rapid and efficie...
ORGANISM(S): Homo sapiens 
2006-02-24 | E-GEOD-4175 | biostudies-arrayexpress
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