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ProteomicsDifferential centrifugation coupled to affinity purification-mass spectrometry of mcherry-ATG8E
Autophagosomes are double-membraned vesicles that traffic harmful or unwanted cellular macromolecules to the vacuole for recycling. Although autophagosome biogenesis has been studied extensively, autophagosome maturation, i.e., delivery and fusion with the vacuole, remains largely unknown in plants....
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-10-18 | PXD031787 | Pride
Arabidopsis Lc-msms Autophagosomes Affinity purification 
ProteomicsShuffled ATG8 interacting motifs form an ancestral bridge between UFMylation and C53-mediated autophagy
UFMylation mediates the covalent modification of substrate proteins with UFM1 (Ubiquitin- fold modifier 1) and regulates the selective degradation of endoplasmic reticulum (ER) via autophagy (ER-phagy) to maintain ER homeostasis. Specifically, collisions of the ER-bound ribosomes trigger ribosome UF...
ORGANISM(S): Chlamydomonas reinhardtii 
2022-11-22 | PXD038157 | Pride
Chlamydomonas reinhardtii Rpl26 Er-phagy Ufmylation 
ProteomicsA cross-kingdom conserved ER-phagy receptor maintains endoplasmic reticulum homeostasis during stress
We have identified a potential selective autophagy receptor protein in Arabidopsis thaliana, C53/AT5G06830/ERP1, that is recruited to ER upon ER-stress activation and induces autophagosome formation. By affinity proteomics IP/MS with ATG8A and E, we identified this new ER-phagy receptor, which is al...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2021-09-09 | PXD019988 | Pride
Arabidopsis thaliana Whole seedlings Tmt-11plex Tunicamycin Er-phagy Ip/ms Er-stress 
ProteomicsBiochemical basis of Exo70 specialization
Molecular machines comprised by multiple subunits govern essential processes in the cell. Understanding how these complexes evolve is crucial to comprehend the evolution of cellular complexity. However, our understanding on the molecular mechanisms that allow multimeric complexes to change and acqui...
ORGANISM(S): Marchantia polymorpha subsp. ruderalis 
2025-12-01 | PXD051853 | Pride
Exocyst  cell trafficking  evolution  marchantia polymorpha  biomolecular condensates  protein complexes. 
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