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ProteomicsLC–MS/MS Proteomic Profiling of PEAK3 S69 Phosphorylation in PEAK3 Mutants
This project aims to investigate how different PEAK3 mutations affect phosphorylation at serine 69 (S69). To this end, we performed proteomic analyses on immunoprecipitated (IP) PEAK3 samples from both wild-type and mutant constructs. Peptides containing phosphorylated and non-phosphorylated S69 wer...
ORGANISM(S): Homo sapiens (Human) 
2026-03-16 | PXD071947 | Pride
Proteomics Peak3 Breast cancer Lc-ms/ms 
ProteomicsLC-MSMS based (phospho)proteomics on gastric cancer cell lines
We exploit a large panel of gastric cancer cell lines and three powerful (phospho)proteomic workflows, including tyrosine phosphorylation profiling, to provide the characterization of the gastric cancer proteome. The resulting datasets are used individually and in an integrated manner to generate no...
ORGANISM(S): Homo sapiens (Human) 
2024-12-16 | PXD048538 | Pride
Lc-msms Proteomics Gastric cancer Tyrosine phosphorylation profiling Phosphoproteomics 
ProteomicsIdentification of biological pathways and processes regulated by NEK5 in breast epithelial cells via an integrated proteomic approach
Specific members of the Nima-Related Kinase (NEK) family have been linked to cancer development and progression, and a role for NEK5, one of the least studied members, in breast cancer has recently been proposed. However, while NEK5 is known to regulate centrosome separation and mitotic spindle asse...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD035322 | Pride
Neks  nek5  kinase  breast cancer  proteomics  bioid 
ProteomicsThe pseudokinase NRBP1 activates Rac/Cdc42 via P-Rex1 to drive oncogenic signalling in triple negative breast cancer
In an effort to identify oncogenic protein kinase drivers in triple negative breast cancer (TNBC), we undertook mass spectrometry (MS)-based proteomic profiling across a large panel of TNBC cell lines. This revealed marked variation in expression of the multidomain pseudokinase Nuclear Receptor Bind...
ORGANISM(S): Homo sapiens (Human) 
2023-05-05 | PXD032168 | Pride
G proteins Proteomics Signal transduction Scaffold proteins 
ProteomicsProteomic profiling of human prostate cancer-associated fibroblasts identifies LOXL2 promotes tumour migration in the extracellular matrix
In prostate cancer (PC), cancer-associated fibroblasts (CAFs) exhibit contrasting biological properties to non-malignant prostate fibroblasts (NPFs) and promote tumorigenesis. Resolving intercellular signaling pathways between CAFs and prostate tumor epithelium may offer novel opportunities for rese...
ORGANISM(S): Homo sapiens (Human) 
2019-05-24 | PXD010611 | Pride
Phosphoproteome Fibroblast Loxl2 Prostate cancer 
ProteomicsCharacterization of the Src-regulated kinome identifies SGK1 as a key mediator of Src-induced transformation
Despite significant progress, our understanding of how specific oncogenes transform cells is still limited and likely underestimates the complexity of downstream signalling events. Herein, we describe a novel, integrated approach that addresses this knowledge gap. This utilizes mass spectrometry-bas...
ORGANISM(S): Homo sapiens (Human) 
2019-01-14 | PXD010687 | Pride
Breast cancer Cancer Src Kinome 
ProteomicsCharacterization of PEAK3 pseudokinase as a pro-migratory and -invasive member of New Kinase Family 3
The New Kinase Family 3 (NKF3) of pseudokinases comprises PEAK1 and PEAK2 as well as the recently-identified PEAK3. PEAK1/2 play fundamental roles in regulating tyrosine kinase signal output and oncogenesis, while PEAK3 remains poorly-characterized. Here we demonstrate that PEAK3 undergoes homotypic...
ORGANISM(S): Homo sapiens (Human) 
2022-03-14 | PXD023687 | Pride
Nkf3/ peak1 (sgk269)/ peak2 (sgk223 Pragmin)/ peak3/brest cancer 
ProteomicsCharacterisation of pancreatic ductal adenocarcinoma subtypes by global phosphotyrosine profiling
Here we use high-resolution mass spectrometry (MS) to characterise the magnitude and complexity of global tyrosine phosphorylation PDAC. We profiled the endogenous tyrosine phosphorylation-based signaling in 36 cell lines from two cell line populations, quantifying around 2,000 tyrosine phosphorylat...
ORGANISM(S): Homo sapiens (Human) 
2016-06-09 | PXD003198 | Pride
Phosphoproteome Pancreatic cancer Shotgun Phosphotyrosine Phosphoproteomics Subtypes 
ProteomicsSgK269 Interactome - Homo- and Heterotypic Association Regulates Signalling by the SgK269/PEAK1 and SgK223 Pseudokinases
Anti Flag IP of Flag-SgK269 and control IP from MCF10A EcoR cells.
ORGANISM(S): Homo sapiens (Human) 
2016-08-24 | PXD004250 | Pride
Pseudokinase sgk269 Protein-protein interaction 
ProteomicsHs_MitoticExit_Phos_Silac_IT
Global phosphoproteomic mapping of early mitotic exit in human hela cells, ITCID
ORGANISM(S): Homo Sapiens (human) 
2015-12-31 | PAe005294 | PeptideAtlas
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