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The goal of this study was to profile the total proteome and transcriptome of the established medulloblastoma cell lines, Daoy and UW228, using label-free nano-LC-MS/MS-based quantitative proteomics and high-throughput RNA sequencing (RNA-Seq), coupled with pathway analysis to identify differentiall...
ORGANISM(S): Homo sapiens (Human) 
2017-10-27 | PXD002659 | Pride
Using measurements of absolute protein and mRNA concentrations in cellular lysate from a Daoy medulloblastoma cell line, we quantitatively evaluate the influence of absolute transcript levels and a large number of sequence-encoded regulatory elements on steady-state human protein expression levels. ...
ORGANISM(S): Homo sapiens 
Bmi-1 and Mel-18 are close structural homologues that belong to the polycomb group (PcG) of transcriptional regulators of homeotic gene expression in development. They are believed to stably maintain repression of gene expression by altering the state of chromatin at specific promoters. A number of...
ORGANISM(S): Homo sapiens 
Aberrant activation of Hedgehog (HH) signaling has been identified as a key etiologic factor of many human malignancies. Signal strength, target gene specificity, and oncogenic activity of HH signaling profoundly depend on interactions with other pathways such as epidermal growth factor receptor-med...
ORGANISM(S): Homo sapiens 
Induction of the transcription factor Sox2 from a doxycycline-inducible promoter in iSox2-DAOY medulloblastoma cells. Affymetrix microarrays were used to characterize the gene expression profile of iSox2-DAOY cells in the absence and presence of doxycycline. Human DAOY medulloblastoma cells were eng...
ORGANISM(S): Homo sapiens 
Culturing of Daoy cells in astrocyte conditioned media
To understand the role of LIN28B protein in medulloblastoma by siRNA-mediated silencing of LIN28B in Daoy cells and analysing the differentially-regulated proteins and pathways.
ORGANISM(S): Homo sapiens (Human) 
2025-05-06 | PXD022481 | Pride
Expression profile of shNT and shPTX3 DAOY cells
This SuperSeries is composed of the following subset Series: GSE34050: ChIP-chip from DAOY cells stably expressing HA-MXD3 with anti-HA GSE34100: Expression profiling of MXD3 stable cell lines Refer to individual Series
ORGANISM(S): Homo sapiens 
DAOY-Notch1/Notch2 knockout: transcriptome comparison
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