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TranscriptomicsDual RNA-seq of M. tuberculosis H37Rv infecting macrophage-like THP-1 cells during 48h
Transcriptional changes during early infection of macrophage-like THP-1 cell line with pathogenic bacterium Mycobacterium tuberculosis. RNAseq samples were taken at 0h (THP-1 cells growing in the RPMI medium), and after 4h, 24h and 48h post infection. Bacterial enrichment was performed to increase t...
ORGANISM(S): Homo sapiens 
2017-08-01 | E-MTAB-5287 | biostudies-arrayexpress
MetabolomicsHigh-resolution extracted ion chromatography, a new tool for metabolomics and lipidomics using a second-generation orbitrap mass spectrometer
Most analytical methods in metabolomics are based on one of two strategies. The first strategy is aimed at specifically analysing a limited number of known metabolites or compound classes. Alternatively, an unbiased approach can be used for profiling as many features as possible in a given metabolom...
2012-02-14 | MTBLS4 | MetaboLights
UnknownRNA viruses, M satellites, chromosomal killer genes, and killer/nonkiller phenotypes in the 100-genomes S. cerevisiae strains.
Not available
S-EPMC10542562 | biostudies-literature
TranscriptomicsThe role of insulin resistance in experimental diabetic retinopathy - genetic and molecular aspects
Patients with long-duration diabetes develop cardiovascular complications resulting in highly increased mortality and complications which affect the kidneys, eyes and peripheral nerves associated with high morbidity. Among the diabetic complications, damage in the eye, diabetic retinopathy, is the m...
ORGANISM(S): Rattus norvegicus 
2017-03-31 | E-MTAB-5563 | biostudies-arrayexpress
ProteomicsIdentification and characterization of proteins that form the inner core Ixodes scapularis tick attachment cement layer.
Ixodes scapularis and other hard ticks are long-term blood feeders. This feeding behavior is facilitated by a tick secreted bio adhesive (tick cement) that attaches tick mouthparts to skin tissue and prevents the host from dislodging the tick from its feeding site. Understanding tick cement formatio...
ORGANISM(S): Ixodes scapularis (Black-legged tick) (Deer tick) Oryctolagus cuniculus (Rabbit) 
2023-03-11 | PXD038685 | Pride
Ixodes scapularis tick cement Laser capture micro dissection Lc-ms/ms 
ProteomicsHypoxia induces Foxn1 and Mmp-9 expression in keratinocytes: proteomics analysis of mouse keratinocytes transiently activated by Foxn1
Recent studies have shown that transcription factor Foxn1 expressed in keratinocytes is involved in skin wound healing process, yet how Foxn1 functions remains largely unknown. Proteomic analysis using a two-dimensional differential gel electrophoresis (2D-DIGE) technique coupled with MALDI-TOF/TOF ...
ORGANISM(S): Mus musculus (Mouse) 
2018-04-09 | PXD007843 | Pride
Proteomics Skin Wound healing Hypoxia Mmp-9 Foxn1 
UnknownLactobacillus plantarum prevents and mitigates alcohol-induced disruption of colonic epithelial tight junctions, endotoxemia, and liver damage by an EGF receptor-dependent mechanism.
Not available
S-EPMC6181630 | biostudies-literature
ProteomicsApplication of Two-Dimensional Difference Gel Electrophoresis to Identify Protein Changes between Center, Margin, and Adjacent Non-Tumor Tissues Obtained from Non-Small-Cell Lung Cancer with Squamous Cell Carcinoma Subtype
Lung cancer is responsible for the most cancer-related mortality worldwide and the mechanism of its development is poorly understood. Proteomics has become a powerful tool offering vital knowledge related to cancer development. Using a two-dimensional difference gel electrophoresis (2D-DIGE) approa...
ORGANISM(S): Homo sapiens (Human) 
2022-05-20 | PXD032962 | Pride
Cancer Tissue Lung Scc Human Squamous cell carcinoma 
ProteomicsApplication of Two-Dimensional Difference Gel Electrophoresis to Identify Protein Changes between Center, Margin, and Adjacent Non-Tumor Tissues Obtained from Non-Small-Cell Lung Cancer with Adenocarcinoma Subtype
Lung cancer is responsible for the most cancer-related mortality worldwide and the mechanism of its development is poorly understood. Proteomics has become a powerful tool offering vital knowledge related to cancer development. Using a two-dimensional difference gel electrophoresis (2D-DIGE) approa...
ORGANISM(S): Homo sapiens (Human) 
2022-05-20 | PXD032736 | Pride
Cancer Lung tissue Human 2d-dige Adenocarcinoma 
ProteomicsLoss of fertilizing ability of sturgeon spermatozoa cryopreserved with the use of DMSO is not related to impairment of sperm motility but alteration of proteins involved in acrosome reaction, egg recognition and early stages of embryo development
Background: The cryopreservation of semen is crucial for conserving genetic diversity and reproductive success in endangered species like the Siberian sturgeon. However, this process can cause cryodamage, affecting quality and protein profile of spermatozoa. While cryoprotectants like dimethyl sulfo...
ORGANISM(S): Acipenser baerii 
2024-08-10 | PXD052170 | Pride
Extracellular medium Mass spectrometry Cryopreservation Acipenser baerii Semen Proteome 
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