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ProteomicsEngineering CRBN for high throughput screening and molecular glue discovery
The majority of clinical degraders utilize an immunomodulatory imide drug (IMiD)-based derivative, that directs their target to the E3 ligase receptor Cereblon (CRBN), however, identification of new IMiD molecular glue substrates has remained underexplored. To tackle this, we design human CRBN const...
ORGANISM(S): Homo sapiens (Human) 
2024-12-06 | PXD056863 | Pride
Coip Flagcrbn Wcp 
ProteomicsCoronavirus PLPro interactome AP-MS data
Main proteases and papain-like proteases (PLpro) are essential coronaviral enzymes required for polypeptide processing during viral maturation. PLpro additionally cleave host cellular proteins to evade anti-viral immune responses. Here, we provide biochemical, structural and functional characterizat...
ORGANISM(S): Homo sapiens (Human) 
2020-08-05 | PXD018983 | Pride
Isgylation Ap-ms Sars-cov-2 Plpro 
ProteomicsA Lysosome membrane regeneration pathway depends on TBC1D15 and autophagic lysosomal reformation proteins
Acute lysosomal membrane damage reduces the cellular population of functional lysosomes. However, these damaged lysosomes have a remarkable recovery potential independent of lysosomal biogenesis and remain unaffected in TFEB/TFE3-depleted cells. We combined proximity labelling based proteomics, bioc...
ORGANISM(S): Homo sapiens (Human) 
2023-03-07 | PXD040191 | Pride
Autophagy Lysosomal regeneration Lysosomes Autophagic lysosomal reformation Tbc family of proteins 
ProteomicsSIK2-dependent Phosphoproteome and Interactome upon Salmonella infection
HeLa cells transfected with non-targeting control- and anti-SIK2 siRNA were left uninfected or infected with Salmonella and lysed 30 min pi. Following tryptic-digest peptides were labeled using TMT-pro 16-plex and phosphopeptides enriched. The endogenously tagged SIK2-HA IP workflow. Hela wild type ...
ORGANISM(S): Homo sapiens (Human) 
2021-05-06 | PXD021859 | Pride
Homo sapiens (human) 
ProteomicsPhosphoproteome upon Salmonella infection
HCT116 cells were grown in SILAC-DMEM (arginine and lysine-free medium), supplemented with dialyzed FCS (10%) and lysine [74µg/ml] and arginine [42µg/ml] as “light K0, R0”, “medium K4, R6” or “heavy K8, R10”. Cells were left uninfected or infected with Salmonella and lysed 30 min or 2h post-infectio...
ORGANISM(S): Homo sapiens (Human) 
2021-05-06 | PXD023703 | Pride
Human 
ProteomicsPLEKHM1 is a direct target for both mTOR and MAPK pathways
The lysosome is a cellular signalling hub at the point of convergence of endocytic and autophagic pathways, where the contents are degraded and recycled. Previously, we identified the adaptor protein PLEKHM1, which facilitates the fusion of endocytic and autophagic vesicles with the lysosome. It is ...
ORGANISM(S): Homo sapiens (Human) 
2021-01-19 | PXD021199 | Pride
Human Autophagy Gfp-trap pull-down Silac Phosphorylation 
ProteomicsFamotidine targets Toll-like receptor 3 signaling in SARS-CoV2 infection
Caco2 cells were pretreated with 100µM histamine and 50µM Famotidine for 12h followed by infection with SARS-CoV2 for 24h. After that proteome was analysed.
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD025703 | Pride
Famotidine Sars-cov2 Toll-like receptor Histamine Anti-viral signaling 
ProteomicsRufy4 exists as two translationally regulated isoforms, that localize to the mitochondrion in activated macrophages
We report here that RUFY4, a newly characterized member of the "RUN and FYVE domain-containing" family of proteins previously associated with autophagy enhancement, is highly expressed in alveolar macrophages (AM). We show that RUFY4 interacts with mitochondria upon stimulation by microbial-associat...
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD026728 | Pride
Lps Mitophagy Rufy4 Alveolar macrophages Skp 
ProteomicsMass-Spectrometric analysis of SdeA Ubiquitin modification and Ubiquitination
Conventional ubiquitination involves the ATP-dependent formation of amide bonds between the ubiquitin C-terminus and primary amines in substrate proteins. Recently, SdeA, an effector protein of pathogenic Legionella pneumophila, was shown to mediate NAD-dependent and ATP-independent ubiquitin transf...
ORGANISM(S): Homo sapiens (Human) 
2016-12-07 | PXD005240 | Pride
Legionella pneumophila Etd Orbitrap fusion 
ProteomicsAnalysis of SdeA modification by SidJ
Legionella pneumophila is a pathogenic gram-negative bacterium that evades host defense pathways to establish its intracellular replicative niche1. SidE family (SidEs) effectors mediate phosphoribosyl (PR)-dependent ubiquitination of host substrates and promote bacterial infection. Legionella effect...
ORGANISM(S): Homo sapiens (Human) Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513) 
2019-07-16 | PXD014362 | Pride
Glutamylation Legionella pneumophila 
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