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ProteomicsEngineering CRBN for high throughput screening and molecular glue discovery
The majority of clinical degraders utilize an immunomodulatory imide drug (IMiD)-based derivative, that directs their target to the E3 ligase receptor Cereblon (CRBN), however, identification of new IMiD molecular glue substrates has remained underexplored. To tackle this, we design human CRBN const...
ORGANISM(S): Homo sapiens (Human) 
2024-12-06 | PXD056863 | Pride
Coip Flagcrbn Wcp 
ProteomicsCoronavirus PLPro interactome AP-MS data
Main proteases and papain-like proteases (PLpro) are essential coronaviral enzymes required for polypeptide processing during viral maturation. PLpro additionally cleave host cellular proteins to evade anti-viral immune responses. Here, we provide biochemical, structural and functional characterizat...
ORGANISM(S): Homo sapiens (Human) 
2020-08-05 | PXD018983 | Pride
Isgylation Ap-ms Sars-cov-2 Plpro 
ProteomicsA Lysosome membrane regeneration pathway depends on TBC1D15 and autophagic lysosomal reformation proteins
Acute lysosomal membrane damage reduces the cellular population of functional lysosomes. However, these damaged lysosomes have a remarkable recovery potential independent of lysosomal biogenesis and remain unaffected in TFEB/TFE3-depleted cells. We combined proximity labelling based proteomics, bioc...
ORGANISM(S): Homo sapiens (Human) 
2023-03-07 | PXD040191 | Pride
Autophagy Lysosomal regeneration Lysosomes Autophagic lysosomal reformation Tbc family of proteins 
ProteomicsSIK2-dependent Phosphoproteome and Interactome upon Salmonella infection
HeLa cells transfected with non-targeting control- and anti-SIK2 siRNA were left uninfected or infected with Salmonella and lysed 30 min pi. Following tryptic-digest peptides were labeled using TMT-pro 16-plex and phosphopeptides enriched. The endogenously tagged SIK2-HA IP workflow. Hela wild type ...
ORGANISM(S): Homo sapiens (Human) 
2021-05-06 | PXD021859 | Pride
Homo sapiens (human) 
ProteomicsPhosphoproteome upon Salmonella infection
HCT116 cells were grown in SILAC-DMEM (arginine and lysine-free medium), supplemented with dialyzed FCS (10%) and lysine [74µg/ml] and arginine [42µg/ml] as “light K0, R0”, “medium K4, R6” or “heavy K8, R10”. Cells were left uninfected or infected with Salmonella and lysed 30 min or 2h post-infectio...
ORGANISM(S): Homo sapiens (Human) 
2021-05-06 | PXD023703 | Pride
Human 
ProteomicsSerine ubiquitination of SNAREs generates bacterial vacuoles and prevents their lysosomal fusion
Here we show that Legionella SidE effectors mediate phosphoribosyl-linked serine ubiquitination (PR-Ub) of the autophagic SNARE proteins STX17 and SNAP29. On the one hand, PR-Ub modification of STX17 enhances its interaction with ATG14L, which drives the recruitment of ER membranes to the bacterial ...
ORGANISM(S): Homo sapiens (Human) 
2025-05-14 | PXD058184 | Pride
Legionella pneumophila Xenophagy Syntaxin17 Ubiquitin Autophagy 
ProteomicsPLEKHM1 is a direct target for both mTOR and MAPK pathways
The lysosome is a cellular signalling hub at the point of convergence of endocytic and autophagic pathways, where the contents are degraded and recycled. Previously, we identified the adaptor protein PLEKHM1, which facilitates the fusion of endocytic and autophagic vesicles with the lysosome. It is ...
ORGANISM(S): Homo sapiens (Human) 
2021-01-19 | PXD021199 | Pride
Human Autophagy Gfp-trap pull-down Silac Phosphorylation 
ProteomicsGABARAP-L2 WT, S87/88D or A interactomes
TBK1 phosphorylates GABARAP-L2 S87/88. In order to test if this phosphomimetic mutation in GABARAP-L2 changes its binding to other proteins a mass spectrometry based interactome study was performed.
ORGANISM(S): Homo sapiens (Human) 
2019-11-06 | PXD015155 | Pride
Gabarap-l2 
Proteomicsendogenous TBK1-mediated GABARAP-L2 and LC3C phosphorylation site identification
In order to identify TBK1-mediated GABARAP-L2 and LC3C phosphorylation sites, cells were treated with TBK1 or control siRNA and TBK1 kinase activity was induced by treatment with CCCP.
ORGANISM(S): Homo sapiens (Human) 
2019-11-06 | PXD015077 | Pride
Lc3c Tbk1 Gabarap-l2 
ProteomicsTBK1-mediated GABARAP-L2 phosphorylation site identification
In order to identify TBK1-mediated GABARAP-L2 phosphorylation sites, proteins were overexpressed in SILAC labelled cells.
ORGANISM(S): Homo sapiens (Human) 
2019-11-06 | PXD015075 | Pride
Tbk1 Gabarapl2 
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