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ProteomicsDOT1 Like Histone Lysine Methyltransferase (DOT1L) and Menin 1 (MEN1) interaction proteomics in breast cancer cell nuclei
Estrogen Receptor alpha (ERα) is a ligand-inducible transcription factor that mediates estrogen signaling in hormone-responsive breast cancer (BC) and is the primary target of specific anticancer therapies that although effective can generate resistance phenomena that represents a crucial problem in...
ORGANISM(S): Homo sapiens (Human) 
2022-08-12 | PXD026864 | Pride
Breast cancer Interaction proteomics Dot1l Endocrine therapy resistance Men1 
TranscriptomicsExpression changes after loss of Dot1l in murine MLL-AF9 leukemia cells
MLL-fusions may induce leukemogenic gene expression programs by recruiting the histone H3K79 methyltransferase to MLL-target promoters. We evaluated gene expression changes after cre-mediated loss of Dot1l in leukemia cells obtained from mice injected with MLL-9 transformed lineage negative bone mar...
ORGANISM(S): Mus musculus 
2011-09-12 | E-GEOD-25911 | biostudies-arrayexpress
ProteomicsInhibition of DOT1L increases osteoclast fusion and resorption and aggravates bone loss
Osteoclasts are absorptive cells and play a critical role in homeostatic bone remodeling and pathological bone resorption. Emerging evidence suggests an important role for epigenetic regulation of osteoclastogenesis. In this study, we investigated the role of DOT1L, which regulates gene expression e...
ORGANISM(S): Mus musculus (Mouse) 
2018-01-24 | PXD006448 | Pride
Proteomics Osteoclast Dot1l Osteoporosis 
ProteomicsEstrogen Receptor alpha and Histone Lysine Methyltransferase DOT1L chromatin bound interactome
Estrogen Receptor alpha (ERα) is a ligand-inducible transcription factor that mediates estrogen signaling in hormone-responsive breast cancer (BC) and is the primary target of specific anticancer therapies. Although ERα blockade with these drugs is effective, the development of a resistance to treat...
ORGANISM(S): Homo sapiens (Human) 
2019-02-09 | PXD010314 | Pride
Estrogen receptor alpha Estrogen signaling Breast cancer chromatin interactome Dot1l Lc-ms/ms 
ProteomicsDOT1L AP-MS in human K562 cells to characterize its associated proteins
we performed large-scale co-immunoprecipitation (co-IP) using a DOT1L antibody with nuclear extract from K562 cells, and characterized immunoprecipitated proteins by mass spectrometry.
ORGANISM(S): Homo sapiens (Human) 
2021-06-18 | PXD024874 | Pride
Ap-ms K562 Dot1l 
GenomicsAF9 Links Histone Acetylation to DOT1L-Mediated H3K79 Methylation
The recognition of modified histones by “reader” proteins constitutes a key mechanism regulating gene expression in the chromatin context. Compared with the great variety of readers for histone methylation, few protein modules that recognize histone acetylation are known. Here we show that the evolu...
ORGANISM(S): Homo sapiens 
2014-12-11 | E-GEOD-60366 | biostudies-arrayexpress
ProteomicsTracking DOT1L Methyltransferase Activity by Stable Isotope Labelling Using a Selective Synthetic Co-Factor
Histone methylation is a post-translational modification (PTM) regulating gene expression. We developed an isotopically labelled analogue of co-factor S-adenosyl-L-methionine (13CD3-BrSAM), with selectivity for the histone lysine methyltransferase DOT1L, permitting tracking of methylation activity b...
ORGANISM(S): Homo sapiens (Human) 
2024-06-28 | PXD052790 | Pride
Dot1l methylation epigenetics histones 
TranscriptomicsGene expression changes upon Dot1L knockdown and Dot1L inhibitor treatment during reprogramming
Through a loss-of-function approach, we identified that inhibition of the histone methyltransferase, Dot1L, accelerated somatic cell reprogramming, significantly increased the yield of induced pluripotent stem (iPS) cell colonies, and substituted for Klf4 and c-Myc in the reprogramming cocktail. To ...
ORGANISM(S): Homo sapiens 
2012-03-01 | E-GEOD-29253 | biostudies-arrayexpress
TranscriptomicsThe Histone Methyltransferases MLL1 and DOT1L Cooperate with Meningioma-1 to Induce AML [Mouse Dot1l ko RNA-seq]
Purpose: To characterize transcriptional changes associated with homozygous inactivation of Dot1l or Mll1 in MN1 driven AML Methods: We sequenced mRNA from murine LSK-cells transformed using forced expression of MN1 (MSCV-MN1-IRES-GFP), and transduced with Cre-vector to inactivate either Dot1l or Ml...
ORGANISM(S): Mus musculus 
2016-01-12 | E-GEOD-76748 | biostudies-arrayexpress
ProteomicsDOT1L regulates pericentromeric heterochromatin
Many repetitive DNA elements are packaged in heterochromatin, but depend on occasional transcription to maintain long-term silencing. The factors that promote transcription of repeat elements in heterochromatin are largely unknown. Here, we show that DOT1L, a histone methyltransferase that modifies ...
ORGANISM(S): Mus musculus (Mouse) 
2023-05-26 | PXD028824 | Pride
Mescs Embryo Mass spectrometry Dot1l Chromatin Pericentromere 
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