Sort   by:  
 Page size 
UnknownGlobal analysis of protein degradation in prion infected cells.
Not available
S-EPMC7329860 | biostudies-literature
UnknownMulti-modal Proteomic Characterization of Lysosomal Function and Proteostasis in Progranulin-Deficient Neurons.
Not available
S-EPMC9980118 | biostudies-literature
Proteomicsdynamic SILAC CGR8 mESC DMSO/CHX
mESCs were cultured at least 2 weeks in medium medium: N2B27 for SILAC (DMEM/F12 for SILAC (AthenaES), Neurobasal for SILAC (AthenaES), Sodium Pyruvate (40 mg/mL), N2 (1X), B27 (0.5X), Pen/Strep (1%), L-glutamine (2 mM), beta-mercaptoethanol (50 µM)) + 2i/LIF supplemented with (medium) 13C6 15N4 L-a...
ORGANISM(S): Mus musculus (Mouse) 
2025-10-07 | PXD061306 | Pride
Cgr8 Dsilac Mesc 
Proteomicsdynamic SILAC NIH/3T3 treated with DMSO/CHX
NIH/3T3 cells were cultured at least 2 weeks in light medium: DMEM for SILAC (Thermo scientific) supplemented with PS, dialyzed FBS (Thermo scientific), (light) L-Lysine-2HCl (0.666 mM), (light) L-Arginine-HCl (0.399 mM)44, and L-Proline (200 mg/L). Before switching from light to heavy medium, cells...
ORGANISM(S): Mus musculus (Mouse) 
2025-10-07 | PXD061384 | Pride
Mouse Dsilac Chx 
ProteomicsGlobal analysis of protein degradation rates during prion infection
Prion diseases are rare, neurological disorders caused by the misfolding of the cellular prion protein (PrPC). The misfolded conformers aggregate into cytotoxic fibrils (PrPSc) that facilitate the conversion of additional prion proteins into their misfolded form. Intracellular PrPSc aggregates prima...
ORGANISM(S): Mus musculus (Mouse) 
2021-09-08 | PXD014577 | Pride
N2a Lysosome Cell culture Prion Rml 
UnknownMulti-modal proteomic characterization of lysosomal function and proteostasis in progranulin-deficient neurons.
Not available
S-EPMC10655356 | biostudies-literature
ProteomicsProteome birthdating reveals age-selectivity of protein ubiquitination
Within a cell, proteins have distinct and highly variable half-lives. As a result, molecular ages of proteins can range from seconds to years. How the age of a protein influences its environmental interactions is a largely unexplored area of biology. To investigate the age-selectivity of cellular pa...
ORGANISM(S): Homo sapiens (Human) 
2024-06-18 | PXD045886 | Pride
Proteostasis Aging Protein turnover  proteomics Mass spectrometry (ms) Ubiquitin proteasome system (ups) 
ProteomicsMaintenance of neuronal TDP-43 expression requires axonal lysosome transport
TDP-43 mislocalization and pathology occurs across a range of neurodegenerative diseases, but the pathways that modulate TDP-43 in neurons are not well understood. We generated a Halo-TDP-43 knock-in iPSC line and performed a genome-wide CRISPR interference FACS-based screen to identify modifiers of...
ORGANISM(S): Homo sapiens (Human) 
2025-08-01 | PXD063206 | Pride
Ipsc neurons tdp43 
Sort   by:  
 Page size