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E. coli O157:H7 global gene expression profile is altered during growth in the presence of Hep-2 cells, relative to the absence of cells; We used Affymetrix E. coli 2.0 genechips to determine gene expression changes Experiment Overall Design: E. coli O157:H7 total RNA extracts were collected after a...
ORGANISM(S): Escherichia coli O157:H7 
Triclosan is a biocidal active agent commonly found in domestic cleaning products, hand sanitizers, cosmetics and personal care products. It is used to control microbial contamination and has a broad-spectrum of activity against many Gram-positive and Gram-negative bacteria. The development of tricl...
ORGANISM(S): Escherichia coli O157:H7 
We used TMT labelling to set up different experiment channels containing mixtures of the host cell carrier proteome (E coli) and mock biotherapeutic (BSA), to compare with interference detection channel to model and filter interference, a phenomenon where host cell proteins (HCPs) co-elute with high...
ORGANISM(S): Escherichia coli 
2026-01-12 | PXD049970 | Pride
We added increasing amounts of E coli carrier proteome to BSA, our moch recombinant protein to model interference of high-background BSA peaks with smaller peaks of E coli HCPs, using MS2 and MS3 methods to show co-fragmentation and co-elution based on similar m/z ratio. We created an interference d...
ORGANISM(S): Escherichia coli 
2026-01-12 | PXD050183 | Pride
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