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ProteomicsCarrier protein-enabled host cell proteome analysis (CP-HCP) with integrated isobaric tag interference filtering
We used TMT labelling to set up different experiment channels containing mixtures of the host cell carrier proteome (E coli) and mock biotherapeutic (BSA), to compare with interference detection channel to model and filter interference, a phenomenon where host cell proteins (HCPs) co-elute with high...
ORGANISM(S): Escherichia coli 
2026-01-12 | PXD049970 | Pride
Boost Host cell proteins Multi-plexed proteomics Tmt Carrier proteome Lc-ms Co-isolation Interference Co-fragmentation Dda 
ProteomicsCarrier protein-enabled host cell proteome analysis (CP-HCP) with integrated isobaric tag interference filtering
We have created a CP-HCP method to characterise the carrier proteome of a host cell expression system producing recombinant protein biotherapeutic, which can be compared with the testing sample mixture containing trace amounts of host cell proteins against high-background recombinant protein and als...
ORGANISM(S): Escherichia coli 
2026-01-12 | PXD050214 | Pride
Psms Host cell proteins Ms3 Tmt Carrier proteome Multiplexed proteomics Lc-ms Co-isolation Interference Co-fragmentation Dda 
ProteomicsCarrier protein-enabled host cell proteome analysis (CP-HCP) with integrated isobaric tag interference filtering
We added increasing amounts of E coli carrier proteome to BSA, our moch recombinant protein to model interference of high-background BSA peaks with smaller peaks of E coli HCPs, using MS2 and MS3 methods to show co-fragmentation and co-elution based on similar m/z ratio. We created an interference d...
ORGANISM(S): Escherichia coli 
2026-01-12 | PXD050183 | Pride
Host cell proteins (hcps) Peptide-spectrum matches Tmt Co-elution Lc-ms Multiplexed proteomics Tryptic digest Interference Carrier proteome Data dependent acquisition Co-fragmentation 
UnknownClimate drives the geography of marine consumption by changing predator communities.
Not available
S-EPMC7668042 | biostudies-literature
UnknownHLA-DRB1*11 and variants of the MHC class II locus are strong risk factors for systemic juvenile idiopathic arthritis.
Not available
S-EPMC4702958 | biostudies-literature
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