we found eIF4E2 regulates GSK3b kinase activity. And we performed the quantitative iTRAQ-based phosphoproteomic to identify phophosites regulated by eIF4E2-GSK3b pathway
Translation of messenger RNAs (mRNAs) with premature translation termination codons produces truncated proteins with potentially deleterious effects. This is prevented by nonsense-mediated mRNA decay (NMD) of these mRNAs. NMD is triggered by ribosomes terminating upstream of a splice site marked by ...
Affinity purification of EIF4E2 endogenously tagged with PTP. A cell line with inducible GFP-PTP was used as negative control brucei. 3 biological replicates were used for both EIF4E2 and GFP.
Translation of messenger RNAs (mRNAs) with premature translation termination codons produces truncated proteins with potentially deleterious effects. This is prevented by nonsense-mediated mRNA decay (NMD) of these mRNAs. NMD is triggered by ribosomes terminating upstream of a splice site marked by ...
