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Absence of the eisosome causes general transcriptome remodeling of the yeast cell
We investigated the effect of the absence of the eisosome on the overall yeast transcriptome by gene expression profiling analysis. We used data obtained from RNA-seq (polyA enrichment of total RNA isolates) of 3 different strains, specifically wild type (BY4742), a strain missing PIL1 gene that for...
ORGANISM(S): Saccharomyces cerevisiae 
2023-01-31 | GSE211649 | GEO
To identify proteins that localize to MCC/eisosomes in N. crassa, we isolated proteins that co-purified with the core MCC/eisosome protein LSP-1, which was tagged with GFP. Proteins that co-fractionated with LSP-1::GFP were then identified by mass spectrometry.
ORGANISM(S): Neurospora crassa OR74A 
2021-09-09 | PXD020724 | Pride
Eisosomes, stress-sensitive membrane compartments in yeast, are scaffolded by the BAR-domain proteins Pil1 and Lsp1. We isolated near-native eisosomes as helical filaments of Pil1/Lsp1 lattice bound to plasma membrane lipids and solved their structure by helical reconstruction. From the same near-na...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2024-07-18 | PXD050326 | Pride
We developed and validated a workflow in S. cerevisiae combining auxin-inducible protein degradation with LC-MS/MS phosphoproteomics and used it to identify candidate phosphatase substrates during mitosis, when kinase activity is high. Our results revealed that PP2A-Rts1 influences dephosphorylation...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-10-24 | PXD044337 | Pride
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