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ProteomicsIP-MS analysis of RLF/ZFP292-FLAG
We performed immunoprecipitation followed by LC-MS/MS (IP-MS) in mouse embryonic stem cells in which endogenous RLF or ZFP292 was tagged with FLAG. This approach enabled the identification of proteins associated with RLF/ZFP292. The dataset provides a resource to investigate the composition of RLF/Z...
ORGANISM(S): Mus musculus (Mouse) 
2026-03-21 | PXD075910 | Pride
Mescs Rlf Ip-ms Zfp292 
ProteomicsLarge-scale interactome screen for predicted transcription factors in fission yeast using IP-MS.
Large-scale interactome screen for 89 transcription factors (TFs) in fission yeast. TFs are tagged at the endogenous locus with a 3xFLAG affinity epitope. Cells cultured under optimal conditions (30°C, rich medium) were subjected to IP-MS at 150 mM an 500 mM NaCl.
ORGANISM(S): Schizosaccharomyces pombe 927 
2024-08-30 | PXD054070 | Pride
Pulldown Fission yeast Protein interactions Lc-ms Schizosaccharomyces pombe Ip-ms Maxquant Orbitrap fusion lumos Einprot Transcription factors 
ProteomicsAnalysis of HNRNPC Interactome in MDA-MB231 parental and LM2 cells
To define and compare the interactomes of the RNA binding protein HNRNPC in poorly vs. efficiently metastatic breast adenocarcinoma cells, we carried out immunoprecipitation of endogenous HNRNPC from parental MDA-MB231 cells vs. its highly metastatic isogenic derivate, the MDA-MB231-LM2 cells. We us...
ORGANISM(S): Homo sapiens (Human) 
2023-02-19 | PXD029560 | Pride
Ip-ms  lc-ms/ms  label-free quantification 
ProteomicsIdentification of MELK-mediated Cofilin-1 T70 phosphorylation by IP-LC-MS/MS analysis
To investigate whether MELK phosphorylates Cofilin-1 at residue Threonine 70 (T70), we performed a phosphoproteomic validation using immunoprecipitation coupled with mass spectrometry (IP-LC-MS/MS). HEK293T cells were divided into three experimental groups: (1) MOCK control (Empty Vector), (2) MELK ...
ORGANISM(S): Homo sapiens (Human) 
2026-04-01 | PXD074164 | Pride
Cofilin-1 Melk Immunoprecipitation Affinity purification-ms Otssp167 Hek293t T70 phosphorylation Lc-ms/ms 
ProteomicsImmunoprecipitation Mass-Spectrometry (IP-MS) experiment reveals endogenous targets of ASFV pI73R
To characterize the cellular targets of the ASFV pI73R, we carried out an Immunoprecipitation Mass-Spectrometry (IP-MS) experiment. Whole-cell extracts prepared from PAM cells which infected with ASFV I73R-mHA were used to immunoprecipitate endogenous targets pI73R, with a matched IgG serving as a c...
ORGANISM(S): Sus Scrofa Domesticus 
2021-11-29 | PXD030084 |
ProteomicsEDU-IP-MS of ecDNA positive and negative human COLO320, HEK293T and U251 cell lines
In this study, we established ecDNA-containing cell models by either transfecting synthetic circular DNA or excising endogenous chromosomal DNA. We found that ecDNA can be stable maintained in these cell models. By identifying proteins on nascent DNA, we found DNA damage repair pathway was significa...
ORGANISM(S): Homo sapiens (Human) 
2025-04-18 | PXD060725 | Pride
Edu-ip-ms 
ProteomicsIP-MS analysis of RLF/ZFP292/ZFP654-FLAG
We performed immunoprecipitation followed by LC-MS/MS (IP-MS) in mouse embryonic stem cells in which endogenous RLF, ZFP292 or ZFP654 was tagged with FLAG. This approach enabled the identification of proteins associated with RLF/ZFP292/ZFP654. The dataset provides a resource to investigate the compo...
ORGANISM(S): Mus musculus (Mouse) 
2026-03-21 | PXD075871 | Pride
ProteomicsSIK2-dependent Phosphoproteome and Interactome upon Salmonella infection
HeLa cells transfected with non-targeting control- and anti-SIK2 siRNA were left uninfected or infected with Salmonella and lysed 30 min pi. Following tryptic-digest peptides were labeled using TMT-pro 16-plex and phosphopeptides enriched. The endogenously tagged SIK2-HA IP workflow. Hela wild type ...
ORGANISM(S): Homo sapiens (Human) 
2021-05-06 | PXD021859 | Pride
Homo sapiens (human) 
UnknownStreamlined analysis schema for high-throughput identification of endogenous protein complexes.
Not available
S-EPMC2823922 | biostudies-literature
ProteomicsIP-MS of endogenous DNAJC11 in HeLa cells
Immunoprecipitation-Mass Spectrometry of endogenous DNAJC11 in HeLa cells
ORGANISM(S): Homo Sapiens 
2026-02-04 | PXD074066 |
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