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ProteomicsEnhanced RNA Interactome capture (eRIC) and total proteome of mouse brain, kidney and liver
Male mice on a homogenous C57BL6/J genetic background were sacrificed at 11 to 13 weeks of age by cervical dislocation. Brain, kidneys and liver were immediately harvested, flash frozen in liquid nitrogen and stored at -80°C until use. Animal handling was in accordance with guidelines approved by...
ORGANISM(S): Mus musculus (Mouse) 
2023-03-02 | PXD032113 | Pride
Rbp Ric Rna interactome capture Eric Rna binding protein Rbpome 
ProteomicsEnhanced RNA interactome capture (eRIC) of human malignant bone tumor cells and normal bone/mesenchymal cells
Patient-derived bone tumor (osteosarcoma and giant cell tumor of bone) cells, and the normal mesenchymal stem cells and osteoblasts were cultured and subjected to UV crosslinking (UV) at 254 nm or without crosslinking (noUV) as negative controls. Subsequently, RNA-binding proteins (RBPs) were identi...
ORGANISM(S): Homo sapiens (Human) 
2024-02-28 | PXD038185 | Pride
Eric Human Osteosarcoma 
ProteomicsEnhanced RNA Interactome capture (eRIC) and RNA Interactome capture (RIC) of Jurkat cells (DSMZ, ACC-282) exposed to 0.5mM DMOG or vehicle (DMSO) for 6 hours.
1.0 – 1.3 x 10e8 proliferating Jurkat cells at a density of about 1-1.5 x 10e6 cells/mL were employed per sample. Cells were incubated with 0.5mM DMOG (Cayman Chemical Company, 71210) or an equivalent volume of Dimethyl Sulfoxide (DMSO) (vehicle, Merck 1.02950.0500). The DMSO concentration in the...
ORGANISM(S): Homo sapiens (Human) 
2018-09-03 | PXD010942 | Pride
Dmog Ric Eric N6-methyladenosine Epitranscriptome M6a Rna interactome Rbps Rbpome Rna-binding proteins Dimethyloxalylglycine 
ProteomicsPaenibacillus larvae exoproteins of ERIC I-IV genotypes
The following strains of Paenibacillus larvae classified as ERIC I-IV genotypes (pl1-pl4) were used in the study: ERIC I - DSM-7030; ERIC II - DSM-25430; ERIC III - LMG-16252; and ERIC IV - DSM-3615. The 7 biological replicates (a-g) of the exoprotein fractions prepared from each ERIC genotype were ...
ORGANISM(S): Paenibacillus Larvae (ncbitaxon:1464) 
2019-03-27 | MSV000083636 | MassIVE
ProteomicsEnhanced RNA Interactome capture (eRIC) and RNA Interactome capture (RIC) of Jurkat cells (DSMZ, ACC-282).
1.0 – 1.3 x 10e8 proliferating Jurkat cells at a density of about 1-1.5 x 10e6 cells/mL were employed per sample. Cells were irradiated or not with 254 nm UV light and subjected to eRIC or RIC to determine the RNA-bound proteome. Data correspond to two biologically independent experiments.
ORGANISM(S): Homo sapiens (Human) 
2018-09-03 | PXD010941 | Pride
Ric Eric Rna interactome Rbps Rbpome Rna-binding proteins 
ProteomicsGlobal assessment of protein-protein crosslinking in ex vivo eRIC samples
Male mice on a homogenous C57BL6/J genetic background were sacrificed at 11 to 13 weeks of age by cervical dislocation. Kidneys were immediately harvested, flash frozen in liquid nitrogen and stored at -80°C until use. Animal handling was in accordance with guidelines approved by the European Molecu...
ORGANISM(S): Mus musculus (Mouse) 
2023-03-02 | PXD038100 | Pride
Rna interactome capture. Rbp Ric Eric Rna binding protein Rbpome 
UnknownERIC and WGS Typing of Paenibacillus larvae in Slovenia: Investigation of ERIC I Outbreaks.
Not available
S-EPMC8072612 | biostudies-literature
ProteomicsEnhanced interactome capture (eRIC) of steady-state and arsenite stressed HuH7 cell cytoplasmic and nuclear fractions
HuH7 cells were grown under steady-state conditions or exposed 100 µM Na-arsenite for 1h. Subsequently, the cells were irradiated or not with 253 nm UV light (150 mJ/cm^2), processed to nuclear and cytoplasmic fractions, and subjected to eRIC for determining the nuclear and cytoplasmic RNA-binding ...
ORGANISM(S): Homo sapiens (Human) 
2020-04-06 | PXD016735 | Pride
Human Cell culture Arsenite 
GenomicsMetabolic labeling of RNAs uncovers hidden features and dynamics of the Arabidopsis thaliana transcriptome [ERIC]
Metabolic labeling of RNAs uncovers hidden features and dynamics of the Arabidopsis thaliana transcriptome [ERIC]
PRJNA485747 | ENA
TranscriptomicsMetabolic labeling of RNAs uncovers hidden features and dynamics of the Arabidopsis thaliana transcriptome [ERIC]
We provide evidence that 5-EU metabolic labeling of Arabidopsis RNAs can be used for pulse-chase experiments, which allowed us to determine Arabidopsis RNA half-lives genome-wide without chemical inhibition of transcription. Similar to BRIC-Seq, we performed 5-EU IP chase (ERIC)-Seq in seedlings of ...
ORGANISM(S): Arabidopsis thaliana 
2018-10-31 | GSE118461 | GEO
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