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MetabolomicsBacteria regulate aging physiology in microalgae via induced vesicle production and the methionine cycle (Metabolomics profiling of FACS purified EVs)

In annually reoccurring patterns, microalgae form blooms that persist and decline thereby contributing massively to global biogeochemical cycles. The decline of blooms is mainly caused by nutrient limitation and goes ahead with the aging of individual algal cells. Nutrient intake can re-initiate ...

2024-05-07 | MTBLS5401 | MetaboLights
ProteomicsProteomics of FACS-sorted heterogeneous Corynebacterium glutamicum populations
The metabolic status of individual cells in microbial cultures can differ being relevant for biotechnology, environmental and medical microbiology. However, it is hardly understood in molecular detail due to limitations of current analytical tools. Here, we demonstrate that FACS in combination with ...
ORGANISM(S): Corynebacterium glutamicum ATCC 13032 
2017-11-02 | PXD005812 | Pride
Corynebacterium glutamicum Proteome analysis Facs Label-free quantification Cellular heterogeneity Bcaa producer 
TranscriptomicsHES2 embryonic stem cells (ESCs) grown in standard conditions and FACs sorted for GCTM-2 and CD9
HES2 ESCs were grown in standard ES culture conditions. After 1 week, these cells were FACs sorted for the presence of GCTM-2 and CD9. Keywords: Sorted cell comparison Three consecutive passages of HES2 cells were grown and subjected to FACs sorting. For each, 4 sorted populations of cells (p4, p5...
ORGANISM(S): Homo sapiens 
2010-04-08 | E-GEOD-13201 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Zebrafish single cell dissected and FACS sorted embryos to investigate gastrointestinal tract development implicates novel genes and pathways
Zebrafish (Danio rerio) gutGFP transgenic embryos [Tg(XlEef1a1:GFP)s854] were collected at 4 time points: 2 days post fertilization (dpf), 3, dpf, 4 dpf, 6 dpf. Embryos were dissociated into single cells and sorted by FACS based on GFP expression. RNA was extracted from the different cell population...
ORGANISM(S): Danio rerio 
2009-07-19 | E-GEOD-12189 | biostudies-arrayexpress
ProteomicsFACS sorted podocytes, mesangial cells
FACS sorted glomerular kidney cells from a single mouse were subjected to proteomics.
ORGANISM(S): Mus musculus (Mouse) 
2022-10-13 | PXD016237 | Pride
Mesangial cells Podocytes Endothelia 
ProteomicsLC-MS/MS analysis of sorted GIP producing human K-cell organoids
Organoids derived from a human small intestine were altered so that cells producing the GIP peptide would generate a flouresence marker. These cells were then sorted based on the fluorescence in a FACS system and positive cells collected along wiht negative cell population. These cells were then lys...
ORGANISM(S): Homo sapiens (Human) 
2024-11-15 | PXD052659 | Pride
K-cell Peptidomics Gip Facs 
ProteomicsGlobal proteome of Atg5flox:CamKIIα-Cre:tdTomato FACS sorted WT and KO neurons
The experiment was performed to identify autophagy targets in wildtype and autophagy-deficient forebrain excitatory neurons. Therefore, neurons were isolated from the cortex, hippocampus and striatum of 2-3 weeks old Atg5flox/flox:CamKIIα-Cretg/wt:tdTomato+ (KO) and Atg5wt/wt:CamKIIα-Cretg/wt:tdToma...
ORGANISM(S): Mus musculus (Mouse) 
2022-11-15 | PXD031552 | Pride
Atg5 ko In-vivo proteome Neurons Autophagy 
ProteomicsGlobal proteome of Atg5flox:Slc32a1-Cre:tdTomato FACS sorted WT and KO neurons
The experiment was performed to identify autophagy targets in wildtype and autophagy-deficient forebrain inhibitory neurons. Therefore, neurons were isolated from the cortex, hippocampus and striatum of 2-3 weeks old Atg5flox/flox:Slc32a1-Cretg/wt:tdTomato+ (KO) and Atg5wt/wt:Slc332a1-Cretg/wt:tdTom...
ORGANISM(S): Mus musculus (Mouse) 
2022-11-15 | PXD031535 | Pride
Atg5 ko In-vivo proteome Neurons Autophagy 
TranscriptomicsFACS-sorted Pancreatic Adenocarcinoma for Molecular Profiling
Frozen, whole tissue was dissociated and sorted into to tumor and stromal fractions via FACS Keywords: Mapping Array Profiling of tumor and stromal fractions from whole tissue
ORGANISM(S): Homo sapiens 
2010-02-18 | E-GEOD-14579 | biostudies-arrayexpress
TranscriptomicsSmart-seq2 analysis of P17 FACS sorted retinal cells from the Kcng4-cre;stop-YFP X Thy1-stop-YFP Line#1 mice
Four Kcng4-cre;stop-YFP mouse retinas from two mice were dissected, dissociated and FACS sorted, and single cell RNA-seq libraries were generated for 384 single cells using Smart-seq2. Aligned bam files are generated for 383 samples as one failed to align. Four mouse retinas (labeled 1la, 1Ra, and 2...
ORGANISM(S): Mus musculus 
2016-08-25 | E-GEOD-81903 | biostudies-arrayexpress
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