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S-fatty-acylation is the covalent attachment of long chain fatty acids, predominately palmitate (C16:0, S-palmitoylation), to cysteine (Cys) residues via a thioester linkage on proteins. This post-translational and reversible lipid modification regulates protein function and localization in eukaryot...
ORGANISM(S): Homo sapiens (Human) 
2018-03-28 | PXD008398 | Pride
S-fatty-acylation is the covalent attachment of long chain fatty acids, predominately palmitate (C16:0, S-palmitoylation), to cysteine (Cys) residues via a thioester linkage on proteins. This post-translational and reversible lipid modification regulates protein function and localization in eukaryot...
ORGANISM(S): Homo sapiens (Human) 
2018-03-28 | PXD008399 | Pride
DNA methylation is a key epigenetic regulator of mammalian embryogenesis and somatic cell differentiation. Using high-resolution genome-scale maps of methylation patterns, we show that the formation of myelin in the peripheral nervous system, proceeds with progressive DNA demethylation, which coinci...
ORGANISM(S): Mus musculus 
Given our laboratory interest in IFITM3 S-fatty-acylation and antiviral activity, we sought to directly characterize fatty acids that are covalently attached to the Cys residues of IFITM3. IFITM3 comprises two S-fatty-acylation sites (C71 and C72) in proximity of a intramembrane domain, and anothe...
ORGANISM(S): Homo sapiens (Human) 
2018-03-28 | PXD008401 | Pride
Given our laboratory interest in IFITM3 S-fatty-acylation and antiviral activity, we sought to directly characterize fatty acids that are covalently attached to the Cys residues of IFITM3. IFITM3 comprises two S-fatty-acylation sites (C71 and C72) in proximity of a intramembrane domain, and another ...
ORGANISM(S): Homo sapiens (Human) 
2018-03-28 | PXD008400 | Pride
The aim of the study was to adapt the CysPAT technique to efficiently identify and characterize endogenous S-nitrosylation in cells under physiological conditions of an experiment from a small amount of sample. Using a macrophage cell line RAW 264.7 we compared the efficacy of the method in the iden...
ORGANISM(S): Mus musculus (Mouse) 
2022-02-22 | PXD008182 | Pride
S. cerevisiae strain S288C was subjected to CRISPR/Cas9 edition in the HMG-CoA reducatase gene using a modified housekeeping promoter ADH1 incorporating the up-stream region of TEF1 promoter (UADH1). Mutant strain was called S288C-H2.
ORGANISM(S): Saccharomyces cerevisiae S288c 
Arabidopsis thaliana plants from the genotypes Col-0 and ABA2OE (35S:ABA2 overexpressor in Col-0 background) were used. They were grown in a growth chamber for 5 weeks in short day (8/16 light/dark cycle) at 22 degrees, 150 umol*m2*s-1 (fluorescent lights) and 55% RH in 100-ml pots with Levington's ...
ORGANISM(S): Arabidopsis thaliana 
Arabidopsis thaliana plants from the genotypes Col-0 and abi2-2T (triple mutant abi1-2/abi2-2/hab1-1) were used. They were grown in a growth chamber for 5 weeks in short day (8/16 light/dark cycle) at 22 degrees, 150 umol*m2*s-1 (fluorescent lights) and 55% RH in 100-ml pots with Levingtons L2+S. On...
ORGANISM(S): Arabidopsis thaliana 
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