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ProteomicsIdentification of proteins precipitated by means of Acyl-RAC
The objective was to perform protein identification by ESI-LC-MSMS on a sample subjected to Acyl-RAC assay in order to study protein palmitoylation. The user transfected human paxillin (mCherry-paxillin-CAIL) into a mouse cell line and performed Acyl-RAC assay. The aim of this analysis was to determ...
ORGANISM(S): Mus musculus (Mouse) 
2021-03-30 | PXD023810 | Pride
Paxillin Palmitoylation 3t3-nih 
ProteomicsS-acylated and S-palmitoylated proteins in HeLa
S-fatty-acylation is the covalent attachment of long chain fatty acids, predominately palmitate (C16:0, S-palmitoylation), to cysteine (Cys) residues via a thioester linkage on proteins. This post-translational and reversible lipid modification regulates protein function and localization in eukaryot...
ORGANISM(S): Homo sapiens (Human) 
2018-03-28 | PXD008398 | Pride
Hela Label free quatification S-acylation Acyl-rac S-palmitoylation Alk-16 chemical reporter Fatty acylation Lc-ms/ms 
ProteomicsS-acylation and fatty acylation in Naïve and LPS/IFN- gamma activated Raw264.7
The advances in chemical proteomics have significantly expanded our understanding of the diversity and abundance of fatty-acylated proteins in eukaryotes, and reveal novel functions for these lipid protein modifications. Nonetheless, quantitative comparative proteomic analysis of fatty-acylated prot...
ORGANISM(S): Mus musculus (Mouse) 
2018-03-28 | PXD003652 | Pride
Raw264.7 Post translational modifications S-acylation Fatty acylation S-palmitoylation 
ProteomicsIdentification of substrates of ZDHHC19 in mouse haploid spermatids using Acyl RAC
Reversible palmitoylation is catalyzed by palmitoyltransferase(PAT) from the zinc-finger DHHC domain-containing protein (ZDHHC) family. The Zdhhc19 knockout male mice showed infertility and Zdhhc19 deficient sperm showed severe morphological defects. To identify the candidate substrates of ZDHHC19 i...
ORGANISM(S): Mus musculus (Mouse) 
2025-09-10 | PXD057735 | Pride
Spermiogenesis  zdhhc19  palmitoylation 
ProteomicsIdentification of herpes virus proteines palmitoyled in RPE-1 cells
Protein palmitoylation regulates diverse aspects of cellular function and organization and plays a key role in host immune responses to infection. Palmitoylation also modulates the function and localization of virus-encoded proteins. In Serwa et al. (doi:10.1016/j.chembiol.2015.06.024), we employed ...
ORGANISM(S): Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1) Human herpesvirus 2 (strain 186) (HHV-2) (Human herpes simplex virus 2) Human herpesvirus 1 (strain KOS) (HHV-1) (Human herpes simplex virus 1) 
2022-03-02 | PXD002708 | Pride
Palmitoylation Chemical proteomics Tagging by substrate Acyl-rac Herpes virus 
ProteomicsIdentification of palmitoyl protein thioesterase 1 substrates defines roles for synaptic depalmitoylation
We report here the identification of substrates of the depalmitoylating enzyme PPT1 by quantitative mass spectrometry. We utilized a stringent two-step Acyl Resin-Assisted Capture (Acyl RAC) screen in which increased palmitoylation in vivo in PPT1 knockout is the first selection criteria for substra...
ORGANISM(S): Mus musculus (Mouse) 
2022-05-19 | PXD017270 | Pride
Synapse Synaptic cleft Synaptic vesicle endocytosis Neuronal ceroid lipofuscinoisis Synaptic adhesion molecules Ampa receptor Neurodegeneration Palmitoylation Palmitoyl protein thioesterase 1 
ProteomicsMouse Skin LC-MS/MS
Previously, we reported that a null mutation of the Zdhhc13 gene caused by ethylnitrosourea (ENU) mutagenesis in mice resulted in severe systematic phenotypes with amyloidosis, alopecia, dermatitis and osteoporosis. In this study we continued to delineate the pathological mechanism for the dermatiti...
ORGANISM(S): Mus musculus (Mouse) 
2022-02-25 | PXD002958 | Pride
Mouse skin tissue Palmitoylation Lc-ms/ms 
ProteomicsEnrichment of Cysteine S-palmitoylated peptides using Sodium Deoxycholate Acid Precipitation - SDC-ACE
S-palmitoylation is a poorly understood post-translational modification that is gaining more attention as an essential regulator of cellular processes. The reversible nature of S-palmitoylation allows for fine-tuned control of cellular events and adaptation to stimuli. The enrichment of S-palmitoyla...
ORGANISM(S): Mus musculus (Mouse) Acyrthosiphon pisum (Pea aphid) 
2026-02-16 | PXD054682 | Pride
Cysteine palmitoylation  mouse brain palmitoylome  dbdb mice  mouse tissue specific palmitoylation 
UnknownS-acylation of SOD1, CCS, and a stable SOD1-CCS heterodimer in human spinal cords from ALS and non-ALS subjects.
Not available
S-EPMC5264640 | biostudies-literature
UnknownProteomic and Metabolomic Analyses Reveal Contrasting Anti-Inflammatory Effects of an Extract of Mucor Racemosus Secondary Metabolites Compared to Dexamethasone.
Not available
S-EPMC4619718 | biostudies-literature
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