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ProteomicsFormaldehyde derivatization, a Side Reaction During Filter-Aided Sample Preparation
The filter-aided sample preparation (FASP) method has been commonly used for proteomic sample preparation due to its high efficiency and convenience. Herein, we report an overlooked side reaction during FASP method, resulting in a mass increment of 12 Da. We confirmed that the side reaction is cause...
ORGANISM(S): Caenorhabditis elegans 
2020-08-20 | PXD018737 | Pride
Filter-aided sample preparation Side reaction Formaldehyde derivatization 
ProteomicsUltrasonic-based filter aided sample preparation as the general method to sample preparation in proteomics
In this work, we propose a new high-throughput ultrafast method for large scale proteomics approaches by speeding the classic filter aided sample preparation protocol, FASP. The new US-FASP method matches the analytical minimalism roles as time, cost, sample requirement, reagent consumption, energy ...
ORGANISM(S): Mus musculus (Mouse) Escherichia coli 
2020-06-08 | PXD018360 | Pride
Mus musculus Label-free quantification E. coli Ultrasonic-assisted sample preparation Fasp Lc-ms/ms 
ProteomicsValidation of filter aided sample preparation (FASP) approach for quantitative plant shotgun proteomics
Gel-free LC-based shotgun proteomics represents the current gold standard of proteome analysis due to its outstanding throughput, analytical resolution and reproducibility. Thereby, the efficiency of sample preparation, i.e., protein isolation, solubilization and proteolysis, directly affects the co...
ORGANISM(S): Bos taurus (Bovine) Pisum sativum (Garden pea) Arabidopsis thaliana (Mouse-ear cress) Homo sapiens (Human) 
2023-03-11 | PXD025897 | Pride
Plant proteomics Filter aided sample preparation (fasp) Shotgun proteomics Lc-ms Phenol extraction Label-free quantification Detergent-assisted proteolysis Sodium dodecyl sulfate 
ProteomicsFilter aided, single tip (FAST) method for high throughput, ultrasensitive proteomics sample preparation reveals proteome asymmetry in Xenopus embryos
We report a filter aided, single tip (FAST) method for ultrasensitive proteomic sample preparation. To minimize sample loss and contamination, the method reduces the surface area of the filter to ~0.1 mm2, the total volume of reagents to < 10 μL, and the number of sample transfer steps to two. 25,88...
ORGANISM(S): Homo sapiens (Human) Xenopus laevis (African clawed frog) 
2022-07-12 | PXD015175 | Pride
Single blastomere Sample preparation Ultrasensitive proteomics 
ProteomicsProteomic Analysis of the Human Amniotic Mesenchymal Stromal Cell Secretome by integrated approaches via Filter-Aided Sample Preparation
LC-MS proteomic analysis using top-down and bottom-up combined strategies via Filter-Aided Sample Preparation (FASP) was applied to the secretome of mesenchymal stromal cells isolated from the amniotic membrane of human term placenta in the attempt to define the profile of its intact as well as dige...
ORGANISM(S): Homo sapiens (Human) 
2024-10-25 | PXD055623 | Pride
Filter-aided sample preparation Proteomics Human amniotic mesenchymal stromal cells Secretome 
ProteomicsSensitivity and protein digestion course of proteomic Filter Aided Sample Preparation
1. Sensitivity of FASP was tested using SDS lysates from HeLa cells and mouse brain. Peptides were analyzed using a QExactive HF-X instrument. We found that FASP allows effective processing samples at the low and sub-microgram range. 2. Whole cell lysates of Hela cells were processed with FASP using...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2019-11-12 | PXD014288 | Pride
Fasp Sample preparation 
ProteomicsSample preparation procedure for mass spectrometric analysis of plant histones
The protocol for preparing samples of plant histones prior to LC-MS/MS analysis was established, using a filter-aided sample preparation (FASP) technique to remove natural contaminants and chemical additives incompatible with MS-based procedures. The protocol consists of nuclei isolation, histone ex...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-09-20 | PXD010102 | Pride
Arabidopsis thaliana Mass spectrometry Post-translational modifications Epigenetics Histone derivatization Filter-aided sample preparation 
ProteomicsFilter-aided sample preparation depends on filter unit shape
One of the main goals of shotgun proteomics studies is to identify by mass spectrometry as many proteins as possible, often from low-protein samples, in order to help answer important biological questions. All experimental steps need to be optimized to achieve a thorough proteomic analysis. One of ...
ORGANISM(S): Homo sapiens (Human) 
2020-11-05 | PXD003400 | Pride
Human Fasp Lc-ms/ms 
ProteomicsProteomic Sample Preparation in the Low Microgram Range – A Critical Evaluation of Current Workflows
Efficient and reproducible sample preparation is a prerequisite for any robust and sensitive quantitative bottom-up proteomics workflow. Over the past years, several protocols been specifically developed for the preparation of samples, which are limited in quantity. In the present study, we carried ...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2018-05-11 | PXD006760 | Pride
Single-pot solid-phase-enhanced sample preparation (sp3) Filter-aided sample preparation (fasp) In-stagetip digestion (ist) Sample preparation Bottom-up proteomics 
ProteomicsLarge-scale filter-aided sample preparation method for the analysis of the ubiquitinome
Protein ubiquitination regulates key cellular functions including protein homeostasis and signal transduction. The digestion of ubiquitinated proteins with trypsin yields a glycine-glycine remnant bound to the modified lysine residue (K-ε-GG) that can be recognized by specific antibodies for immunoa...
ORGANISM(S): Homo sapiens (Human) 
2017-03-30 | PXD005421 | Pride
Ubiquitinome Large-scale Portein digestion Post-translational modifications Lfasp Proteome 
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