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ProteomicsSPROX full curve on E. coli lysate
To probe the thermodynamic stability of proteins within the E. coli proteome through proteomic technique SPROX.
ORGANISM(S): Escherichia coli 
2023-01-03 | PXD035834 | Pride
E. coli Sprox Stability 
ProteomicsChemical Denaturation and Protein Precipitation (CPP) on E. coli lysate
Chemical Denaturation and Protein Precipitation (CPP) was performed on an E. coli lysate in order to determine the precipitaiton midpoint for the proteome
ORGANISM(S): Escherichia coli 
2023-01-03 | PXD036219 | Pride
Cpp E. coli Precipitation 
ProteomicsLarge-Scale Analysis of Breast Cancer-Related Conformational Changes in Proteins using Limited Proteolysis
Conformational changes in proteins can lead to disease. Thus, methods for identifying conformational changes in proteins can further improve our understanding and facilitate detection of disease states. Here we combine limited proteolysis (LiP) with Stable Isotope Labeling with Amino Acids in Cell ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-29 | MSV000080770 | MassIVE
ProteomicsMetal-induced Protein Precipitation in E. coli
Using a range of copper we have utilized Metal-induced Protein Precipitaiton (MiPP) technique to elucidate the copper midpoints of the proteins found in an E. coli proteome. Using bottem-up proteomics techniques and TMT-10 plex isobaric mass-tags we were able to use MiPP to classify proteins based o...
ORGANISM(S): Escherichia coli 
2023-01-03 | PXD035711 | Pride
Precipitiation Sprox Cpp Metal E. coli Copper 
ProteomicsOne-pot comparative analysis and characterization of MCF-7 and MCF-10A cell lines
A comparison of different energetics based techniques for the characterization of two mammalian breast cell lines, MCF-7 a luminal A breast cancer cell line and MCF-10A a normal human breast cell line. The techniques of stability of proteins from rates of oxidation (SPROX), thermal proteome profilin...
ORGANISM(S): Homo sapiens (Human) 
2023-02-22 | PXD036268 | Pride
Thermodynamics Thermal denaturation Chemical denaturation Protein folding 
ProteomicsDiscovery of the Xenon-Protein Interactome using Large-Scale Measurements of Protein Folding and Stability
The intermolecular interactions of noble gases in biological systems are associated with numerous biochemical responses, including apoptosis, inflammation, anesthesia, analgesia, and neuroprotection. The molecular modes of action underlying these responses are largely unknown. This is in large part ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-07-20 | PXD029353 | Pride
Noble gases Protein stability Sprox Limited proteolysis 
ProteomicsGlobal Analysis of Protein Folding Thermodynamics for Disease State Characterization, MCF7 vs MDAMB231
Protein biomarkers can be used to characterize and diagnose disease states such as cancer. They can also serve as therapeutic targets. Current methods for protein biomarker discovery, which generally rely on the large-scale analysis of gene and/or protein expression levels, fail to detect protein b...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-29 | MSV000080761 | MassIVE
ProteomicsGlobal Analysis of Protein Folding Thermodynamics for Disease State Characterization, MCF7 vs MCF10A
Protein biomarkers can be used to characterize and diagnose disease states such as cancer. They can also serve as therapeutic targets. Current methods for protein biomarker discovery, which generally rely on the large-scale analysis of gene and/or protein expression levels, fail to detect protein b...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-29 | MSV000080760 | MassIVE
ProteomicsGlobal Analysis of Protein Folding Thermodynamics for Disease State Characterization, MCF7 vs MDAMB231
Protein biomarkers can be used to characterize and diagnose disease states such as cancer. They can also serve as therapeutic targets. Current methods for protein biomarker discovery, which generally rely on the large-scale analysis of gene and/or protein expression levels, fail to detect protein b...
ORGANISM(S): Homo sapiens (Human) 
2015-04-10 | PXD001848 | Pride
Breast cancer Proteomics Mass spectrometry Chemical denaturation Silac Mda-mb-231 Mcf-10a Mcf-7 Protein folding 
ProteomicsGlobal Analysis of Protein Folding Thermodynamics for Disease State Characterization, MCF7 vs MCF10A
Protein biomarkers can be used to characterize and diagnose disease states such as cancer. They can also serve as therapeutic targets. Current methods for protein biomarker discovery, which generally rely on the large-scale analysis of gene and/or protein expression levels, fail to detect protein b...
ORGANISM(S): Homo sapiens (Human) 
2015-04-10 | PXD001847 | Pride
Breast cancer Proteomics Mass spectrometry Chemical denaturation Silac Mda-mb-231 Mcf-10a Mcf-7 Protein folding 
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