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MetabolomicsLegume rhizodeposition promotes nitrogen fixation by soil microbiota under crop diversification

Biological nitrogen fixation by free-living bacteria and rhizobial symbiosis with legumes plays a key role in sustainable crop production. Here, we study how different crop combinations influence the interaction between peanut plants and their rhizosphere microbiota via metabolite deposition and ...

2024-03-19 | MTBLS6537 | MetaboLights
MetabolomicsImpacts of fixation processing workflows on the volatile and non-volatile metabolomic profiles of Gougunao green tea

This study compared four fixation processing workflows and their effects on the volatile and non-volatile metabolomic profiles of Gougunao green tea: fully mechanical fixation (M1), mechanical-manual hybrid fixation (M2), manual-mechanical hybrid fixation (M3), and fully manual fixation (M4). An ...

2026-07-17 | MTBLS15065 | MetaboLights
MetabolomicsCoupled nitrogen fixation and substrate stabilization drive ecological strategy shifts during microbial succession

Background:

Primary succession is predicted to proceed through shifts from stress-tolerant to competitive to cooperative ecological strategies as resource availability increases, yet molecular evidence for these transitions in microbial systems remains limited. Here, we used an integrated m...

2026-03-12 | MTBLS14041 | MetaboLights
MetabolomicsEnhanced photosynthetic CO2 fixation in mutualistic Chlorella sp. and Mesorhizobium loti co-cultures

It was further confirmed the stimulatory effect of M. loti-derived eCA on microalgae, highlighting key carbon metabolic pathways, including the Calvin cycle, TCA cycle and antioxidant defense response.

2025-01-20 | MTBLS11422 | MetaboLights
TranscriptomicsFormalin Fixation at Low Temperature Better Preserves Nucleic Acid Integrity
INTRODUCTION. Fixation with formalin, a widely adopted procedure to preserve tissue samples, leads to extensive degradation of nucleic acids and thereby compromises procedures like microarray-based gene expression profiling. We hypothesized that RNA fragmentation is caused by activation of RNAses du...
ORGANISM(S): Homo sapiens 
2011-09-04 | E-GEOD-27175 | biostudies-arrayexpress
MetabolomicsDissolved metabolites from the Bermuda Atlantic Time-series Study: 2016-2019

Organic carbon in seawater plays a significant role in the global carbon cycle. The concentration and composition of dissolved organic carbon, operationally defined in this project as organic carbon that passes through a 0.2 µm filter, reflect the actions of the biological community and chemical ...

2022-02-09 | MTBLS2356 | MetaboLights
TranscriptomicsTranscription profiling of femoral fracture healing in Rattus norvegicus subjects comparing intramedullary nailing, screw and plate fixation, and sham surgery
This is a study of femoral fracture healing in female rats 16 weeks old at fracture to compare intramedullary nailing, screw and plate fixation, and sham surgery. The sham surgery group received a surgical exposure of the femur, but no fracture, no plate, and no nail. Samples were collected at 1 d...
ORGANISM(S): Rattus norvegicus 
2007-11-03 | E-GEOD-1685 | biostudies-arrayexpress
TranscriptomicsGenes differentially expressed in wild-type Medicago truncatula plants during nodulation
We used an Affymetrix oligonucleotide microarray consisting of 9,935 tentative consensus (TC)sequences, which are based on cDNA libraries (Mitra et al., 2004 [PMID:15220482]). We examined gene expression of wild-type M. truncatula plants after inoculation with wild-type S. meliloti at 1 d, 4 d, 7d,...
ORGANISM(S): Medicago truncatula 
2011-03-26 | E-GEOD-28173 | biostudies-arrayexpress
ProteomicsFormaldehyde fixation helps preserve the proteome state during single-cell proteomics sample processing and analysis
Mass spectrometry-based single-cell proteomics (SCP) is gaining momentum but remains limited to a few laboratories due to the high costs and specialized expertise required. The ability to send samples to specialized core facilities would benefit non-specialist labs and popularize SCP for biological ...
ORGANISM(S): Homo sapiens (Human) 
2026-02-09 | PXD056327 | Pride
Single-cell proteomics Formaldehyde fixation Facs One-tip 
ProteomicsMetabolic Remodeling during Nitrogen Fixation in Zymomonas
This project investigates the proteomic remodeling associated with N2 fixation and NH4+ assimilation in Zymomona mobilis. Previous work has elucidated the metabolic and proteomic response of Zymomona mobilis during steady state N2 fixation. These experiments examine the proteomic profile during the ...
ORGANISM(S): Zymomonas mobilis subsp. mobilis ZM4 = ATCC 31821 
2021-10-07 | PXD028526 | Pride
Nitrogen fixation Zymomonas 
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