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ProteomicsFOXP1 is a transcriptional Coactivator of Wnt/β-catenin signaling in Diffuse Large B-cell Lymphoma
We used a mass spectrometry-coupled lentiviral ‘CD-tagging’ mutagenesis approach to identify genes that activate Wnt/β-catenin signaling. Human A375 melanoma cells containing a β-catenin-driven GFP (green fluorescent protein) transcriptional reporter were transduced with CDBF lentivirus. When integr...
ORGANISM(S): Homo sapiens (Human) 
2016-07-05 | PXD001485 | Pride
Flag apms Gain-of-function screening Wnt signaling Foxp1 
ProteomicsChlamydia trachomatis effector Tri1 interacts with TRAF7 to displace native TRAF7 interactors
Chlamydia trachomatis is the leading cause of bacterial sexually transmitted infections in the US and preventable blindness worldwide. This obligate intracellular pathogen replicates within a membrane-bound inclusion, but how it acquires nutrients from the host while avoiding detection by the innate...
ORGANISM(S): Homo sapiens (Human) Chlamydia trachomatis 
2024-05-05 | PXD049432 | Pride
Chlamydia trachomatis Apms 
ProteomicsSILAC APMS analysis of RPAP3 C-term interactome
To get insights into the function of RPAP3, we characterized its partners by performing a proteomic analysis in human cells. We fused the Cterm part of RPAP3 domain to GFP and stably expressed it in HeLa cells using site-specific integration with the Flp-In system. Following differential labeling of...
ORGANISM(S): Homo sapiens (Human) 
2018-06-10 | PXD009518 | Pride
Human Ltq-orbitrap Hela cells Apms Silac 
ProteomicsSILAC APMS analysis of Pih1D3 interactome
To get insights into the function of Pih1D3 protein, we characterized its partners by performing a proteomic analysis in human cells. We fused this domain to GFP and stably expressed it in HeLa cells using site-specific integration with the Flp-In system. Following differential labeling of GFP-Pih1D...
ORGANISM(S): Homo sapiens (Human) 
2018-06-10 | PXD009499 | Pride
Human Ltq-orbitrap Hela cells Apms Silac 
ProteomicsSILAC APMS analysis of CCDC103 interactome
To get insights into the function of CCD103, we characterized its partners by performing a proteomic analysis in human cells. We fused this domain to GFP and stably expressed it in HeLa cells using site-specific integration with the Flp-In system. Following differential labeling of GFP-CCDC103 and c...
ORGANISM(S): Homo sapiens (Human) 
2018-06-08 | PXD009498 | Pride
Human Ltq-orbitrap Hela cells Apms Silac 
ProteomicsSILAC APMS analysis of Pih1D2 interactome
To get insights into the function of Pih1D2 protein, we characterized its partners by performing a proteomic analysis in human cells. We fused this domain to GFP and stably expressed it in HeLa cells using site-specific integration with the Flp-In system. Following differential labeling of GFP-Pih1D...
ORGANISM(S): Homo sapiens (Human) 
2018-06-10 | PXD009520 | Pride
Human Ltq-orbitrap Hela cells Apms Silac 
ProteomicsSILAC APMS analysis of DNAAF2 interactome
To get insights into the function of DNAAF2 protein, we characterized its partners by performing a proteomic analysis in human cells. We fused this domain to GFP and stably expressed it in HeLa cells using site-specific integration with the Flp-In system. Following differential labeling of GFP- DNAA...
ORGANISM(S): Homo sapiens (Human) 
2018-06-10 | PXD009501 | Pride
Human Ltq-orbitrap Hela cells Apms Silac 
ProteomicsSILAC APMS anlysis of TSSC4 interactome
U5 snRNP is a complex particle essential for RNA splicing. U5 snRNPs undergo an intricate biogenesis that ensures that only a fully mature particle assembles into a splicing competent U4/U6•U5 tri-snRNP and enters the splicing reaction. During splicing, U5 snRNP is substantially rearranged and leave...
ORGANISM(S): Homo sapiens (Human) 
2021-06-11 | PXD024929 | Pride
Apms  silac  immunoprecipitation 
ProteomicsIdentification and Characterization of MCM3 as a KEAP1 Substrate
KEAP1 is a substrate adaptor protein for a CUL3-based E3 ubiquitin ligase. Ubiquitylation and degradation of the antioxidant transcription factor NRF2 is considered the primary function of KEAP1; however, few other KEAP1 substrates have been identified. Because KEAP1 is altered in a number of human ...
ORGANISM(S): Homo sapiens (Human) 
2017-10-12 | PXD003929 | Pride
Hek293t Di-gly Apms Silac Label free quantification 
Proteomics293T cell mitotic AP-MS anti-SIRT6-FLAG 201113
The study found reciprocal regulation between SIRT6 and APC/C. While SIRT6 is ubiquitinated by APC/C and degraded, CDH1, a co-activator of APC/C, is also deacetylated by SIRT6 and degraded.
ORGANISM(S): Homo sapiens (Human) 
2021-09-09 | PXD022500 | Pride
Ap-ms Mitosis 293t 
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