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ProteomicsGetting more out of FLAG-Tag co-immunoprecipitation mass spectrometry experiments using FAIMS.
Co-immunoprecipitation of proteins coupled to mass spectrometry is critical for the understanding of protein interaction networks. In instances where a suitable antibody is not available, it is common to graft synthetic tags onto target protein sequences and allowing the use of commercially availabl...
ORGANISM(S): Homo sapiens (Human) 
2023-02-21 | PXD028965 | Pride
Faims Co-ip mass spectrometry Flag tag Proteomics Ion mobility 
ProteomicsIdentification of host interactors of Brucella BT4E effector proteins by Flag-tag affinity purification coupled with LC-MS/MS in HEK293T cells
This study aimed to systematically identify host cellular proteins that interact with newly discovered Brucella abortus type IV secretion system (T4SS) effector proteins, designated BT4E3, BT4E4, BT4E19, BT4E32, BT4E33, BT4E37, and BT4E43. To achieve this, we employed an affinity purification–mass s...
ORGANISM(S): Homo sapiens (Human) 
2026-02-13 | PXD068987 | Pride
Brucella bt4e effector proteins Hek293t Lc-ms/ms Flag-tag affinity purification 
UnknownTaking down the FLAG! How insect cell expression challenges an established tag-system.
Not available
S-EPMC3368911 | biostudies-literature
ProteomicsArabidopsis Circadian Clock RVE8 LNK1 LNK2 Interactome LC-MS
The timing of many molecular and physiological processes in plants occurs at a specific time of day. The circadian MYB-like transcription factor REVEILLE 8 (RVE8) interacts with its transcriptional coactivators NIGHT LIGHT INDUCIBLE AND CLOCK REGULATED 1 (LNK1) and LNK2 to promote the expression of ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2023-04-24 | PXD034089 | Pride
Arabidopsis thaliana Affinity purification Circadian clock Lcms 
ProteomicsSFB-tagged(S-protein tag, Flag epitope tag, and streptavidin-binding peptide tag) RNF4 wild-type and CS mutant LC-MSMS
To elucidate the role of RNF4 in DNA replication and fork reversal, we performed tandem affinity purification (TAP) using a HEK293T cell line that stably expresses SFB-tagged(S-protein tag, Flag epitope tag, and streptavidin-binding peptide tag) wild-type and CS mutant to isolate proteins that assoc...
ORGANISM(S): Homo sapiens (Human) 
2022-08-11 | PXD028397 | Pride
Human rnf4 wt and cs lc-msms 
UnknownStructural Analysis of the Effect of a Dual-FLAG Tag on Transthyretin.
Not available
S-EPMC7171973 | biostudies-literature
ProteomicsThe HP1 protein interactome of mouse embryonic stem cells
The family of Heterochromatin Protein 1 (HP1) consists of highly conserved proteins, which have important functions in the nucleus of eukaryotic cells. In mammals there are three HP1 paralogs: HP1(alpha), Hp1(beta), and Hp1(gamma)They are encoded by the Cbx5, Cbx1, and Cbx3 genes, respectively. Hp1 ...
ORGANISM(S): Mus musculus (Mouse) 
2018-05-22 | PXD006226 | Pride
Lc-orbitrap fusion Mouse es-cells Tandem purification Chd4 Maxquant. Hp1 Cbx3 Adnp Cbx1 Hp1 Flag-avi-tag Cbx5 Hp1 
ProteomicsEnrichment of NICD4 interacting proteins by three different methods.
There are several methods to bring down the bait and interactors from the cell lysates, each with its own advantages and disadvantages. Recently, the most commonly used method is one step AP, TAP and proximity-labeling. In order to analyze the Strengths and weaknesses of the three methods, fusion pr...
ORGANISM(S): Homo sapiens (Human) 
2022-08-12 | PXD031772 | Pride
Sfb-tap Nicd4 Flag tag ap Turboid. 
ProteomicsIdentification of host interactors of Brucella BT4E effector proteins by Flag-tag affinity purification coupled with LC-MS/MS in HEK293T cells
This study aimed to systematically identify host cellular proteins that interact with newly discovered Brucella abortus type IV secretion system (T4SS) effector proteins, designated BT4E3, BT4E4, BT4E19, BT4E32, BT4E33, BT4E37, and BT4E43. To achieve this, we employed an affinity purification–mass s...
ORGANISM(S): Homo sapiens (Human) 
2026-02-15 | PXD074457 | Pride
Homo sapiens 
ProteomicsPurification of endogenously FLAG-HA-tagged KAT6B from E14 ESCs
An N-terminal FLAG-HA tag was introduced into the endogenous Kat6b locus by CRISPR genome editing and purified by FLAG affinity purification using anti-FLAG agarose beads.
ORGANISM(S): Mus musculus (Mouse) 
2025-01-02 | PXD015988 | Pride
Chromatin Transcription Histone acetylation Embryonic stem cells Differentiation Bivalent domains Histone methylation 
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