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ProteomicsAn HDX-MS optimised approach to characterise nanobodies as tools for biochemical and structural studies
Given the far-reaching applications of nanobodies, a stream-lined approach to characterizing nanobodies tailored to its intended application is warranted. Camelids can produce a large number of antibodies that can bind to a host of epitopes spanning the entire protein of interest, making identificat...
ORGANISM(S): Homo sapiens (Human) 
2021-09-22 | PXD025207 | Pride
Hdx-ms Pi3k Nanobody 
ProteomicsHydrogen Deuterium Exchange of PDK1
This project consisted of three HDX-MS experiments. First, we compared the dimeric PDK1(SKD-PIF) to monomeric PDK1(SKD) and mapped the differences in deuterium incorporation onto the dimer model. We then compared the deuterium incorporation kinetics for the kinase (PDK1(SKD)) and PH (PDK1(PH) domain...
ORGANISM(S): Homo sapiens (Human) 
2022-05-20 | PXD027401 | Pride
Hdx-ms pdk1 hdx 
ProteomicsIn vitro reconstitution of Sgk3 activation by phosphatidylinositol-3-phosphate
Serum- and glucocorticoid-regulated kinase 3 (Sgk3) is activated by the phospholipid 19 phosphatidylinositol-3-phosphate (PI3P) downstream of growth factor signaling and 20 by Vps34-mediated PI3P production during autophagy. Upregulation of Sgk3 activity 21 has been linked to a number of human cance...
ORGANISM(S): Homo sapiens (Human) 
2021-09-22 | PXD025327 | Pride
Kinase Sgk3 Hdx-ms Pi3p 
ProteomicsStructure of the phosphoinositide 3-kinase p110-p101 complex reveals molecular mechanism of GPCR activation
The class IB phosphoinositide 3-kinase (PI3K), PI3K, is a master regulator of immune cell function, and is a promising drug target for both inflammatory diseases and cancer. Critical to PI3K function is the association of the p110 catalytic subunit to either a p101 or p84 regulatory subunit, which ...
ORGANISM(S): Homo sapiens (Human) 
2021-09-22 | PXD025209 | Pride
Hdx Cryo-em Gpcr Pik3g 
UnknownPhase II trial of cetuximab, gemcitabine, and oxaliplatin followed by chemoradiation with cetuximab for locally advanced (T4) pancreatic adenocarcinoma: correlation of Smad4(Dpc4) immunostaining with pattern of disease progression.
Not available
S-EPMC3157965 | biostudies-literature
ProteomicsHDX-MS of TRAPPII reveals a conserved Rab binding interface
We used hydrogen deuterium exchange mass spectrometry (HDX-MS) to define the interface of TRAPPII with three Rab GTPases (Rab1, Rab11, Rab43). These experiments were performed in the presence of EDTA to generate a nucleotide free stabilised Rab-GEF complex. Significant decreases in deuterium incorpo...
ORGANISM(S): Homo sapiens (Human) 
2020-12-11 | PXD020890 | Pride
Hdx-ms Trappii Gef Rab 
ProteomicsTargeting of disease-linked mutations in PI3K gamma
Using hydrogen deuterium exchange mass spectrometry (HDX-MS) and molecular dynamics we have identified mutation specific conformational changes, and provide a molecular framework for how they alter kinase activity.Many of the activating conformational changes mimicked previously described changes th...
ORGANISM(S): Homo sapiens (Human) 
2021-03-26 | PXD021132 | Pride
Molecular dynamics Kinase assay Hdx-ms Inhibitor Pi3k gamma Pi3k 
ProteomicsHDX-MS analysis of PLIN3 membrane binding
Hydrogen deuterium exchange mass spectrometry of PLIN3 in the presence of three different membrane vesicles to analyze structural changes induced by membrane binding.
ORGANISM(S): Homo sapiens (Human) 
2023-04-14 | PXD025717 | Pride
Hdx Hdx-ms Plin3 
UnknownReduced stomatal density in bread wheat leads to increased water-use efficiency.
Not available
S-EPMC6760291 | biostudies-literature
UnknownMultiparametric magnetic resonance for the non-invasive diagnosis of liver disease.
Not available
S-EPMC3865797 | biostudies-literature
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