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ProteomicsComposition of Arabidopsis RNA exosomes having incorporated wild type or mutated myc-tagged RRP41 proteins
RRP41 is one of the nine subunits constituting the core complex of the eukaryotic RNA exosome. Data presented here allowed a proteomic characterization of the interactome of wild type or mutated myc-tagged RRP41 proteins incorporated into the RNA exosome. Flowers from myc-tagged RRP41 expressing lin...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-01-02 | PXD007984 | Pride
Arabidopsis Rna degradation Rrp41 Rna exosome 
ProteomicsRST1 and RIPR connect the cytosolic RNA exosome to the SKI complex in Arabidopsis
The RNA exosome is a key 3’-5’ exoribonuclease with evolutionary conserved structure and roles. Its cytosolic functions require the co-factors SKI7 and the Ski complex. Here we demonstrate by co-purification experiments that the ARM repeat protein RESURRECTION1 (RST1) and RST1 INTERACTING PROTEIN (R...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2019-07-17 | PXD013435 | Pride
Rst1 Exo9 Rna exosome Ski7 Ski complex Exosome complex 
ProteomicsCell type-specific control of secondary cell wall formation by Musashi-type translational regulators in Arabidopsis
Deciphering the mechanism of secondary cell wall/SCW formation in vascular plants is key to understanding their development and the molecular basis of biomass recalcitrance. A sophisticated network of transcription factors has been implicated in SCW synthesis in plants, but little is known about the...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2023-09-28 | PXD040207 | Pride
Arabidopsis Musashi-type transcriptional regulator Cell wall Interactomic 
ProteomicsThe TUTase URT1 connects decapping activators and prevents the accumulation of excessively deadenylated mRNAs to avoid siRNA biogenesis
Uridylation is a widespread modification destabilizing eukaryotic mRNAs. Yet, molecular mechanisms underlying TUTase-mediated mRNA degradation remain mostly unresolved. Here, we report that the Arabidopsis TUTase URT1 participates in a molecular network connecting several translational repressors/de...
ORGANISM(S): Nicotiana tabacum (Common tobacco) 
2020-12-01 | PXD022676 | Pride
Arabidopsis Tutase Deadenylation Dcp5 Rna degradation Gigyf Nanopore drs Decapping Uridylation 
TranscriptomicsGlobal view of mRNA uridylation in Arabidopsis
To obtain a global view of mRNA uridylation in Arabidopsis, we generated TAIL-seq libraries from WT plants, urt1 and xrn4 single mutants, and urt1 xrn4 double mutant. The TAIL-seq protocol was recently developed to deep-sequence the 3' ends of RNAs (Chang et al., 2014). We generated TAIL-seq librari...
ORGANISM(S): Arabidopsis thaliana 
2016-03-11 | E-GEOD-72457 | biostudies-arrayexpress
Transcriptomicstiling hen2 and mtr4-HEN2 is a cofactor of the nuclear exosome in Arabidopsis
til-ath-2011_3 - tiling hen2 and mtr4 - Identification and comparison of the RNA substrates that accumulate in hen2 and mtr4 mutants. Total RNA extracted from WT, hen2 and mtr4 mutants was used for oligo-dT primed cDNA preparation. Samples were hybridised to NimbleGen whole genome tiling arrays. 8 d...
ORGANISM(S): Arabidopsis thaliana 
2013-06-30 | E-GEOD-48178 | biostudies-arrayexpress
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