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Protein phosphorylation has long been recognized as an essential regulator of protein activity, structure, complex formation, and sub-cellular localization among other cellular mechanisms. However, interpretation of the changes in protein phosphorylation is difficult. To address this difficulty, we ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-03-04 | PXD022741 | Pride
Mass spectrometry-based phosphoproteomics has become indispensable for understanding cellular signaling in complex biological systems. Despite the central role of protein phosphorylation, the field still lacks inexpensive, regenerable, and diverse phosphopeptides with ground truth phosphorylation po...
ORGANISM(S): Homo sapiens (Human) 
2022-10-26 | PXD031171 | Pride
Mutations in the E3 ubiquitin ligase Mkrn3 are associated with precocious puberty in humans. In order to determine the targets of Mkrn3, we performed a TMT-based proteomic analysis of Mkrn3 WT vs KO mouse brains.
ORGANISM(S): Mus musculus (Mouse) 
2023-07-20 | PXD027220 | Pride
Tissues were collected during the Amsterdam (Netherlands) study of Carbohydrate Regulation of Lipid Metabolism ("SODA").  Subjects were divided between those with and without Metabolic Dysfunction Associated Steatotic Liver Disease (MASLD); subjects were further divided by insulin resistance as asse...
ORGANISM(S): Homo sapiens (Human) 
2026-01-26 | PXD061142 | Pride
Obesity is a major cancer risk factor, but the underlying molecular mechanisms are not always known. In this study, we look at proteome remodeling in cancer cells with obesity, comparing tumor cells sorted from mice fed high-fat versus control diet. We conducted 10-plex TMT-proteomics on GFP+ MC38 c...
ORGANISM(S): Mus musculus (Mouse) 
2020-12-16 | PXD019495 | Pride
A proteomic comparison of the cerebella of APC7 KO mice versus controls using TMT11-plex reagents
ORGANISM(S): Mus musculus (Mouse) 
2023-03-10 | PXD016683 | Pride
The BioPlex (biophysical interactions of ORFeome-based complexes) network is the result of creating thousands of cell lines with each expressing a tagged version of a protein from the ORFeome collection. Immunopurification of the tagged protein and detection of associated proteins by mass spectromet...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-12-13 | MSV000088555 | MassIVE
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