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Tetracycline inducible, stable HEK-293 cell lines expressing BirA-FLAG-GATAD1 variants were generated using the Flp-In system. Besides WT GATAD1, S102P (ProMut), S102D (AspMut), and S102A (AlaMut)mutant variants were studied. The goal of the experiment was to compare the differences in the interacto...
ORGANISM(S): Homo sapiens (Human) 
2024-02-15 | PXD046950 | Pride
To gain insights into the interactome of wild-type (WT) and S102P mutant GATAD1, we utilized the BioID method, which enables the study of protein-protein interactions. Specifically, we performed BioID proximity labeling experiments in stable Flp-In cells expressing different GATAD1 variants fused to...
ORGANISM(S): Homo sapiens (Human) 
2024-02-15 | PXD043789 | Pride
To explore the role of individual amino acids in the binding of GATAD1 peptide (position 95-109) to 14-3-3 proteins, we conducted an alanine scanning experiment. Each amino acid surrounding the phosphorylation site within the peptide was systematically mutated to alanine, except for the original ala...
ORGANISM(S): Homo sapiens (Human) 
2024-02-15 | PXD043788 | Pride
Genomics
Transcriptome of gene expression after knocking out GATAD1 in MCF-7 cell line
Genomics
Transcriptome of gene expression after knocking out GATAD1 in MCF-7 cell line
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