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ProteomicsGFP Pulldown from A375 cells expression microprotein-GFP candidates
Bottom-up mass spectrometric identification of interaction partners of candidate microproteins-GFP fusions after anti-GFP pulldown and tryptic digest Sample preparation For each of the conditions, we prepared two biological replicates. For each replicate, a confluent T75 flask of cells was pelleted...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-05-12 | MSV000091927 | MassIVE
ProteomicsComplementary proteomics strategies identify the ataxin-1 interactome in Neuro-2a cells
To identify protein partners of ataxin-1 in neuronal cells under control or stress conditions, we use complementary proteomics strategies of proximity-dependent biotin identification (BioID) and affinity purification (via GFP-trap pulldown) in Neuro-2a cells expressing epitope-tagged forms of ataxin...
ORGANISM(S): Mus musculus (Mouse) 
2018-10-18 | PXD010352 | Pride
Gfp-trap Ataxin-1 Bioid Interactome Neuro-2a Lc – msms 
ProteomicsInteractome of human ZCCHC4 - Exploration of the ZCCHC4 interactome through affinity proteomics.
Exploration of the ZCCHC4 interactome through affinity proteomics.
ORGANISM(S): Homo sapiens (Human) 
2019-11-22 | PXD012346 | Pride
Gfp-pulldown Inetractomics Lfq 
ProteomicsPianalto KM et al "Characterization of novel components of the environmental pH-sensing complex in Cryptococcus neoformans"
Three analyses performed by the Duke Proteomics and Metabolomics Shared Resource in support of the project entitled "". Sample ID21519 was called Rra1-GFP, a 1D coomassie-stained gel band from a GFP-Trap pulldown of Rra1-GFP from the membrane protein fraction of Cryptococcus neoformans. Sample ID21...
ORGANISM(S): Cryptococcus Neoformans (ncbitaxon:5207) 
2018-03-06 | MSV000082127 | MassIVE
ProteomicsProteomic analysis of filamin-A, IQGAP1, Rac1 and RCC2 binding partners
GFP GFP pulldown
ORGANISM(S): Homo sapiens (Human) 
2013-07-22 | PRD000726 | Pride
ProteomicsAdenovirus E4ORF1 activates isoform-specific phosphatidylinositol 3-kinase signaling
The human adenovirus serotype 5 E4ORF1 (Ad5E4ORF1) protein promotes primary endothelial cell survival and angiocrine functions by hijacking the cellular phosphatidylinositol 3-kinase (PI3K)/AKT signaling pathway. However, the mechanism by which E4ORF1 activates PI3K in vascular cells remains largely...
ORGANISM(S): Homo sapiens (Human) 
2026-01-05 | PXD069348 | Pride
Adenovirus  akt  angiogenesis  dlg1  e4orf1  endothelium  phosphatidylinositol-3-kinase. 
ProteomicsInteracting partners of Rac1 (P29S/Q61L) compared to constitutively active Rac1 (Q61L)
Interactors of double mutant Rac1 (P29S/Q61L) compared to constitutively active Rac1 (Q61L).
ORGANISM(S): Chlorocebus sabaeus 
2019-11-12 | PXD013941 | Pride
Cos7 cells Rac1 Gfp trap pulldown 
ProteomicsGFP pulldown for the RiboVD project
GFP-tagged viral peptide sequences and their controls were used to pull down interactors from human (uninfected) or green monkey (viral infected) cell lysate. After sample preparation, digestion and bottom-up LC-MS/MS analysis, the enriched proteins were identified.
ORGANISM(S): Homo sapiens (Human) Cercopithecus aethiops (Green monkey) (Grivet) 
2023-03-09 | PXD033874 | Pride
Pulldown Motif Virus Interaction Coronavirus 
ProteomicsTLNRD1-GFP pulldown in HEK cells
In this study, we pull-down human TLNRD1-GFP and GFP from HEK cells. Binding partners were then analysed using mass-spectrometry.
ORGANISM(S): Homo sapiens (Human) 
2024-01-27 | PXD045258 | Pride
Cell lines Gfp trap 
ProteomicsTalin1-GFP pulldown in U2OS cells
In this study, we pull-down human Talin1-GFP and GFP from U2OS cells plated on fibronectin. Binding partners were then analysed using mass-spectrometry.
ORGANISM(S): Homo sapiens (Human) 
2021-05-12 | PXD024634 | Pride
Talin1 Gfp trap U2os 
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