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MetabolomicsPROMIS, global analysis of PROtein-Metabolite Interactions using Size separation in Arabidopsis thaliana
Small molecules not only represent cellular building blocks and metabolic intermediates, but also regulatory ligands and signaling molecules that interact with proteins. Although these interactions affect cellular metabolism, growth, and development, they have been largely understudied. Herein, we d...
2018-07-19 | MTBLS94 | MetaboLights
ProteomicsProtein and metabolite composition of Arabidopsis stress granules
• Stress granules (SGs) are evolutionary conserved aggregates of proteins and untranslated mRNAs formed in response to stress. Despite their importance for stress adaptation, no complete proteome composition has been reported for plant SGs. Herein, we addressed the existing gap. Importantly, we also...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2019-01-07 | PXD011716 | Pride
Cdka1 Arabidopsis thaliana Stress granules proteome Stress response Stress granule metabolome Affinity purification Stress granule extraction 
ProteomicsProtein and metabolite composition of the Arabidopsis stress granules.
Stress granules are evolutionary conserved aggregates of proteins and untranslated mRNAs formed in response to stress. Herein, we report protein composition of the Arabidopsis SGs supporting their role in plant response to stress. Moreover, and to our knowledge for the first time, we provide eviden...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-03-27 | PXD010455 | Pride
Ap-ms Stress granules Protein Metabolite Arabidopsis thaliana Lc-ms/ms 
ProteomicsSeparation and Paired Proteome Profiling of Plant Chloroplast and Cytoplasmic Ribosomes
Conventional preparation methods of plant ribosomes fail to resolve non-translating chloroplast or cytoplasmic ribosome subunits from translating fractions. We established preparation of these ribosome complexes from Arabidopsis thaliana leaf, root, and seed tissues by optimized sucrose density grad...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2021-09-09 | PXD019329 | Pride
Plant proteomics Tissue specific separation Ribosome complexes Ribo-proteome In vivo protein complex stabilization 
ProteomicsHit-Gel: Streamlining in-gel protein digestion for high-throughput proteomics experiments.
Traditional in gel-digestion procedures require extensive sample handling, are prone to contamination and not compatible with high-throughput sample preparation. To address these shortcomings, we have modified the conventional in-gel digestion procedure for high-throughput proteomics studies. The mo...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-10-16 | PXD009510 | Pride
Large-scale In-gel digestion Proteomics Tryptic digest Fractionation Native protein complex Sample preparation High-throughput Lc-ms/ms 
ProteomicsHit-Gel: Streamlining in-gel protein digestion for high-throughput proteomics experiments.
Traditional in gel-digestion procedures require extensive sample handling, are prone to contamination and not compatible with high-throughput sample preparation. To address these shortcomings, we have modified the conventional in-gel digestion procedure for high-throughput proteomics studies. The mo...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-10-16 | PXD009485 | Pride
Large-scale In-gel digestion Proteomics Tryptic digest Fractionation Native protein complex Sample preparation High-throughput Lc-ms/ms 
ProteomicsProtein Complex identification and quantitative complexome by CN-PAGE
The majority of cellular processes are carried out by protein complexes. Various size fractionation methods have previously been combined with mass spectrometry to identify protein complexes. However, most of these approaches lack the quantitative information which is required to understand how chan...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2019-11-12 | PXD010919 | Pride
Cn-page Mass spectrometry Proteomics Protein complexes Protein-protein interactions 
ProteomicsSelective autophagy regulates heat stress memory by NBR1-mediated targeting of HSP90 and ROF1
In nature, plants are constantly exposed to many transient, but recurring, stresses. Thus, to complete their life cycles they require a dynamic balance between capacities to recover following cessation of stress and maintenance of stress memory. Recently, we uncovered a new functional role of autoph...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-02-15 | PXD015027 | Pride
Lc-ms Autophagy Heat stress memory 
ProteomicsPROMIS, global analysis of PROtein-Metabolite Interactions using Size separation in Arabidopsis thaliana
Herein, we describe a method, dubbed PROMIS that allows simultaneous, global analysis of endogenous protein–small-molecule and protein-protein complexes. To this end a cell-free native lysate was subjected to size-exclusion chromatography followed by quantitative metabolomic and proteomic analysis. ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-06-06 | PXD009714 | Pride
Protein-metabolite Size exclusion chromatografy Deconvolution Correlation Interactions 
ProteomicsBoth cold and sub-zero acclimation induce cell wall modification and changes in the extracellular proteome in Arabidopsis thaliana
Cold acclimation (CA) leads to increased plant freezing tolerance during exposure to low, non-freezing temperatures as a result of many physiological, biochemical and molecular changes that have been extensively investigated. In addition, many plant species, such as Arabidopsis thaliana, respond to ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2019-11-12 | PXD010575 | Pride
Apoplast Proteome Sub-zero Freezing tolerance Cell wall Extracellular 
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