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ProteomicsPNET1 is an on-demand component of the nuclear pore complex gating cell proliferation rates
The nuclear pore complex (NPC) is a cornerstone of eukaryotic cell functionality, orchestrating the nucleocytoplasmic shuttling of macromolecules. Here, we report that PNET1, a transmembrane nucleoporin conserved between humans and plants, is a dynamic, adaptable NPC component, exclusively expressed...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2025-03-10 | PXD054475 | Pride
Arabidopsis thaliana Callus Nuclear pore complex 
ProteomicsMembrane protein landscape of plant nuclear envelope and nuclear pore complex
We used biochemical fractionation coupled with the subtractive proteomics to profile PNET proteins. Both the crude NE and the total MM fraction were subject to label-free quantitative mass spectrometry (LFQMS). We also adapted proximity labeling-coupled LFQMS (PL-LFQMS) method to map the PNET proteo...
ORGANISM(S): Arabidopsis Thaliana 
2020-03-11 | PXD015919 |
TranscriptomicsExpression profiling of Col-0 and rps2 treated with Psm:AvrRpt2
Identification of differential gene expression during RPS2-mediated effector-triggered immunity in Arabidopsis Four-week-old leaves of Arabidopsis Col-0 wild type and rps2 mutant plant were infiltrated with Pseudomonas syringae pv. Maculicola carring effector AvrRpt2 at OD 0.01. Samples were collect...
ORGANISM(S): Arabidopsis thaliana 
2016-09-01 | E-GEOD-72742 | biostudies-arrayexpress
ProteomicsKA120 negatively regulate immunity through inhibit MOS 4-associated-complex forming nuclear condensates
To understand how KA120 participates in plant immune regulation as a karyopherin-β, we sought to determine in vivo substrates of KA120. We leveraged an enzyme-catalyzed proximity-labeling strategy, in which we fused KA120 with TurboID. MAC3B could form nuclear condensates in ka120 mutant thus cause...
ORGANISM(S): Arabidopsis Thaliana 
2023-02-23 | PXD040403 |
ProteomicsA multi-omics atlas of the plant nuclear envelope
The nuclear envelope (NE) proteins play a pivotal role in maintaining nuclear integrity and orchestrating nucleocytoplasmic communication. Increasing evidence suggests that NE proteins interact with a wide array of other proteins, implying functions far beyond membrane compartmentalization. However,...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2026-03-06 | PXD071667 | Pride
Arabidopsis Nuclear envelope Nucleus 
ProteomicsA multi-omics atlas of the plant nuclear envelope
The nuclear envelope (NE) proteins play a pivotal role in maintaining nuclear integrity and orchestrating nucleocytoplasmic communication. Increasing evidence suggests that NE proteins interact with a wide array of other proteins, implying functions far beyond membrane compartmentalization. However,...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2026-03-06 | PXD071466 | Pride
Proximity labeling Arabidopsis Nuclear envelope Nucleus 
ProteomicsA plant-specific mitochondria outer membrane protein regulates mitochondrial fusion
Mitochondria are essential organelles in eukaryotes for energy production and are composed by a double-layered membrane structure that contains a smooth mitochondrial outer membrane (MOM) and a highly invaginated mitochondrial inner membrane (MIM). Mitochondria are dynamic with the ability to fuse a...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-09-05 | PXD039252 | Pride
Mitochondria Aggregating vacuole Outer membrane 
ProteomicsGBPL3 is a novel component of the nuclear pore complex and functionally connects the nuclear basket with the nucleoskeleton in plants
The nuclear basket (NB) is an essential structure of the nuclear pore complex (NPC). It serves as a dynamic and multi-functional platform that participates in various critical nuclear processes, including cargo transport, molecular docking, and gene expression regulation. However, the compositional ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-08-25 | PXD032906 | Pride
Turboid Gbpl3 Biotin 
ProteomicsA Conserved Nuclear Membrane Protein As an Essential Component of Plant Nuclear Lamina Is Required for Proper Genome Organization and Activity
Plant conserved nuclear membrane protein PNET2 was identified to associate with nucleoskeleton protein CRWN and KAKU4, nucleosome histone H2. As an essential component of nuclear lamina, PNET2 is required for global transcriptional reprogramming and chromasomal packing, thus to control plant surviva...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2023-03-11 | PXD026924 | Pride
Pnet2 crwn1 kaku4 man1 proximity chromatin 
ProteomicsThe inner nuclear membrane-associated degradation system in plants
The homeostasis of the inner nuclear membrane (INM) proteins plays a critical role in regulating the nuclear structure, genome integrity, and signal transduction. However, little is known about the turnover of INM proteins in higher eukaryotes. Here we demonstrated that the conserved INM protein SUN...
ORGANISM(S): Arabidopsis Thaliana 
2020-03-11 | PXD015920 |
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