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ProteomicsIsolation and whole protein characterization of species A and B bovine rotaviruses from Chinese calves.
Rotaviruses (RVs) account for severe diarrhea in children and young animals globally. In the current study, the fecal samples of diarrheic calves from a beef farm in Inner Mongolia were screened for RVA by ELISA and RT- PCR, followed by culture of three positive RVA samples in the MA-104 cell line. ...
ORGANISM(S): Bos taurus (Bovine) 
2021-01-18 | PXD017047 | Pride
Bovine rotavirus Brb Protein typing Esl-lc- ms/ms Bra 
ProteomicsLabel-free based Proteomic analysis of secreted proteins from Mycoplasma bovis HB0801 and its attenuated strain P150
This study was aimed to perform a comparative analysis on potential secretory proteins between high (HB0801) and low (P150) virulent M.bovis strains. We employed the liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based label-free quantitative proteomic technology to identify and character...
ORGANISM(S): Mycoplasma bovis HB0801 
2021-09-08 | PXD017700 | Pride
Secreted proteins Label-free Mycoplasma bovis 
ProteomicsComprehensive Immunoproteome of Mycoplasma bovis
This study was aimed to elucidate a global antigenic profile of Mycoplasma bovis (M. bovis) with immunoproteomics, immunoinformatics, and gene expression approaches. The extracts of whole-cell proteins and TX-114 membrane fraction of a Chinese strain M. bovis HB0801 were separated with two dimension...
ORGANISM(S): Bos taurus (Bovine) Mycoplasma bovis HB0801 
2016-06-13 | PXD003479 | Pride
Maldi-tof ms Immunoblotting 2-de Immunoinformatics 
ProteomicsThe MbovP280 of mycoplasma bovis immunoprecipitates with the wole cell lysates of bovine macrophage (BoMac),and then LC-MS/MS was used to identify the MbovP280-binding ligands
An IP–MS method was used to screen for MbovP280-binding proteins. Briefly, BoMac cells were cultured, harvested, and lysed in RIPA buffer supplemented with cOmplete Protease Inhibitor Cocktail (Roche, Mannheim, Germany). The whole-cell lysates (400 μg) were incubated with 10 μg of either rMbovP280 o...
ORGANISM(S): Bos taurus (Bovine) 
2021-09-09 | PXD022080 | Pride
Lc-ms/ms Bomac Bovine Ip Mycoplasma bovis Mbovp280 
ProteomicsDifferential expressed proteins between Mycoplasma bovis different strains
Comparing differential expressed proteins between Mycoplasma bovis different strains by using label-free method
ORGANISM(S): Mycoplasma bovis HB0801 
2020-06-15 | PXD017374 | Pride
Differential expressed protein Mycoplasma bovis 
UnknownMycoplasma bovis NADH oxidase functions as both a NADH oxidizing and O2 reducing enzyme and an adhesin.
Not available
S-EPMC5427908 | biostudies-literature
UnknownAssessment of the physicochemical properties and bacterial composition of Lactobacillus plantarum and Enterococcus faecium-fermented Astragalus membranaceus using single molecule, real-time sequencing technology.
Not available
S-EPMC6082834 | biostudies-literature
UnknownTrmFO, a Fibronectin-Binding Adhesin of Mycoplasma bovis.
Not available
S-EPMC5578122 | biostudies-literature
UnknownCalves Infected with Virulent and Attenuated Mycoplasma bovis Strains Have Upregulated Th17 Inflammatory and Th1 Protective Responses, Respectively.
Not available
S-EPMC6770603 | biostudies-literature
UnknownIdentification of potential urine proteins and microRNA biomarkers for the diagnosis of pulmonary tuberculosis patients.
Not available
S-EPMC5893550 | biostudies-literature
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