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ProteomicsProteomic-based identification of oocyte maturation-related proteins in mouse GV oocytes
In this study, we applied 1D SDS-PAGE and RP-LC-MS/MS to investigate the proteins stored in GV mouse oocytes. This high-performance strategy allowed us to define a set of 1405 different mouse GV oocyte proteins. It is confirmed that this study will help us to understand the diverse biological proces...
ORGANISM(S): Mus musculus (Mouse) 
2020-09-17 | PXD014314 | Pride
Mouse Gv oocytes Lc-ms/ms 
ProteomicsTMT-based proteomics analysis of GV, GVBD and MII oocytes
Two replicates of GV, GVBD, and MII oocytes were subjected to the 6-plex TMT labeling, HP-RP fractionation, and LC-MS/MS analysis. Two labeling experiments were performed for the total four replicates of each stage of oocytes.
ORGANISM(S): Mus musculus (Mouse) 
2022-02-15 | PXD018777 | Pride
Lc-msms Mii Oocytes Mouse Gvbd Gv 
TranscriptomicsTranscription profiling of immature germinal vesicle stage oocytes with mature metaphase II oocytes to identify and characterize the changed and stable transcripts during mouse oocyte maturation.
There is massive destruction of transcripts during maturation of mouse oocytes. The objective of this project was to identify and characterize the transcripts that are degraded versus those that are stable during the transcriptionally silent germinal vesicle (GV)-stage to metaphase II (MII)-stage t...
ORGANISM(S): Mus musculus 
2007-11-13 | E-GEOD-5668 | biostudies-arrayexpress
TranscriptomicsMicroarray data of prepubertal, peripubertal, and adult oocytes, and from GV and MII oocytes matured in vivo or in vitro
Expression data from prepubertal, peripubertal, and adult derived mouse oocytes, and from germinal vesicle (GV), in vivo matured, and in vitro matured mouse oocytes. Oocytes derived from prepubertal females, or oocytes matured in vitro, are less developmentally competent compared to adult derived, ...
ORGANISM(S): Mus musculus 
2010-07-12 | E-GEOD-12432 | biostudies-arrayexpress
TranscriptomicsMicroarray profiling of UPF2-deficient mouse GV oocytes.
To study the role of UPF2 during oogenesis, Upf2 was conditionally deleted in growing oocytes. Total RNA samples from UPF2-deficient GV oocytes were subjected to array profiling. The control samples for this experiment can be found in E-MTAB-5056.
ORGANISM(S): Mus musculus 
2019-01-14 | E-MTAB-7064 | biostudies-arrayexpress
ProteomicsQuantitative phosphoproteomics analysis of GV, GVBD and MII oocytes
Five replicates of GV, GVBD, and MII oocytes were subjected to the 15-plex TMT labeling, HP-RP fractionation, and LC-MS/MS analysis. For each replicate, 2,000 oocytes were collected from each of the GV, GVBD, and MII stages. Ti4+-IMAC was used to enrich phosphopeptides.
ORGANISM(S): Mus musculus (Mouse) 
2025-05-13 | PXD050645 | Pride
Phosphoproteomics Mouse Lc-ms/ms Oocyte 
TranscriptomicsTranscription profiling of mouse in vivo matured MII oocytes and fully in vivo grown germinal vesicle oocytes to identify gene transcripts linked to epigenetic reprogramming
To elucidate some of the tools involved in early embryonic reprogramming, the levels of gene transcripts believed to be of importance to epigenetic modifications, and chromatin remodeling were detected by oligonucleotide microarrays in in vivo matured MII oocytes and compared with fully in vivo grow...
ORGANISM(S): Mus musculus 
2007-07-02 | E-MEXP-1146 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse oocytes reveals abundant transcripts from retrotransposons are unstable
One physiological function proposed for RNA interference (RNAi) is to constrain expression of repetitive elements and thereby reduce the incidence of retrotransposition. Consistent with this model is that inhibiting the RNAi pathway results in an increase in expression of repetitive elements in pre...
ORGANISM(S): Mus musculus 
2008-06-13 | E-GEOD-4508 | biostudies-arrayexpress
ProteomicsQuantitative proteomic analysis of both fresh and vitrified porcine GV oocytes during in vitro maturation
In this study, we used tandem mass tag (TMT)-based quantitative approach to acquire proteomic profiles of porcine GV and MII oocytes and MII oocytes vitrified at the GV stage.
ORGANISM(S): Sus scrofa domesticus (domestic pig) 
2021-07-01 | PXD023107 | Pride
Oocytes Tmt Vitrification Pig Ivm 
TranscriptomicsGene Expression Profiles in Immature and In vitro matured bovine Oocytes
In cattle, almost all fully grown vesicle stage oocytes (GV) have the ability to resume meisos, develop to Metaphase II stage (MII), support fertilization and progress through the early embryonic cycles in vitro. Yet without intensive selection, the majority fail to develop to the blastocyst stage. ...
ORGANISM(S): Bos taurus 
2011-04-25 | E-GEOD-23449 | biostudies-arrayexpress
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