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ProteomicsUncover proteins binding HBV rcDNA
To comprehensively uncover host proteins involved in HBV rcDNA repair, we employed a rcDNA repair system, which can simulate the process of cccDNA formation in vitro. Biotinylated rcDNA first bonds with streptavidin magnetic beads and is then incubated with cell extracts of HepG2-NTCP-K7 cells to fa...
ORGANISM(S): Homo sapiens (Human) 
2024-12-18 | PXD053940 | Pride
Hbv rcdna Dna repair Hepatocytes 
ProteomicsIdentification of cellular protein interacting with HBV genome by protein mass spectrometry
: Hepatitis B virus (HBV) remains a major public health threat with more than 296 million people chronically infected worldwide at high risk to develop hepatocellular carcinoma. Current therapies are effective in suppressing HBV replication but rarely lead to cure. The fundamental limitation rests o...
ORGANISM(S): Homo sapiens (Human) 
2024-06-16 | PXD042733 | Pride
Hbv cccdna Mini-chromosome Transcription Therapy 
GenomicsBinding of Nucleolin to HBV cccDNA
Binding of Nucleolin to HBV cccDNA
PRJNA509631 | ENA
ProteomicsInterferon-induced degradation of the hepatitis B virus persistence form, cccDNA, depends on ISG20
Hepatitis B virus (HBV) persists by depositing a covalently closed circular DNA (cccDNA) in the nucleus of infected cells that cannot be targeted by available antivirals. Cytokine treatments can diminish HBV cccDNA via APOBEC3-mediated deamination. Here we show that overexpression of APOBEC3A alone,...
ORGANISM(S): Homo sapiens (Human) 
2021-04-22 | PXD019285 | Pride
Isg20 Apobec3a Hbv Interferon-gamma Cccdna Chronic hepatitis b Interferon-alpha 
GenomicsBinding of Nucleolin to HBV cccDNA
Covalently closed circular DNA (cccDNA) forms the basis for replication and persistence of hepatitis B virus (HBV) in the chronically infected liver. In this study we sought to identify host factors interacting with the HBV genome. Nucleolin (Ncl) was identified as a potential interactor of HBV cccD...
ORGANISM(S): Homo sapiens 
2021-12-31 | GSE123715 | GEO
GenomicsHBV cccDNA Generated Using Single-Stranded M13 Phage DNA for Authentic Minichromosome Functionality
HBV cccDNA Generated Using Single-Stranded M13 Phage DNA for Authentic Minichromosome Functionality
PRJNA1234531 | ENA
UnknownMinicircle HBV cccDNA with a Gaussia luciferase reporter for investigating HBV cccDNA biology and developing cccDNA-targeting drugs.
Not available
S-EPMC5098228 | biostudies-literature
GenomicsTranscription of HBV cccDNA is synchronized at single-cell level and orchestrated by expression of HBx
Transcription of HBV cccDNA is synchronized at single-cell level and orchestrated by expression of HBx
PRJNA658780 | ENA
UnknownDroplet digital PCR assay provides intrahepatic HBV cccDNA quantification tool for clinical application.
Not available
S-EPMC8826402 | biostudies-literature
GenomicsHBV cccDNA Generated Using Single-Stranded M13 Phage DNA for Authentic Minichromosome Functionality
Hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) persists as minichromosomes in the nuclei of hepatocytes and serves as the transcriptional template for viral RNAs. The cccDNA pool, characterized by a low copy number, poses challenges for cccDNA research. To accurately mimic the full-...
ORGANISM(S): Homo sapiens 
2026-03-16 | GSE291625 | GEO
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