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ProteomicsPhosphoproteomic analysis of T cell receptor stimulation-induced changes in human primary CD4+CD25- T cells in the absence or presence of regulatory T cells.
This is an addition to dataset PXD004291. This data were only used for confirmation of certain phosphopetides. The dataset was not included in inital analysis due to suboptimal sample preparation.
ORGANISM(S): Homo sapiens (Human) 
2017-08-28 | PXD006142 | Pride
T-cells Phosphoproteomics 
ProteomicsPhosphoproteomic analysis of T cell receptor stimulation-induced changes in human primary CD4+CD25- T cells in the absence or presence of regulatory T cells.
Regulatory T cells (Tregs) suppress other immune cells and are indispensable for human health, yet the molecular mechanisms of suppression remain incompletely understood. Tregs can suppress proliferation and cytokine production of CD4+CD25- conventional T cells (Tcons). We previously demonstrated th...
ORGANISM(S): Homo sapiens (Human) 
2017-08-28 | PXD004291 | Pride
Co-cultures Regulatory t cells (tregs) Ti-imac Human cd4+ t cells Phosphoproteomics 
ProteomicsTi4+-IMAC label-free quantification
We combine high-resolution mass spectrometry with Ti4+-IMAC phosphopeptide enrichment and label-free quantification to monitor the phosphoproteome of Jurkat T-cells following stimulation by Prostaglandin E2. Jurkat T lymphoma cells were grown in RPMI 1640 medium supplemented with 10% fetal bovine se...
ORGANISM(S): Homo sapiens (Human) 
2014-05-22 | PXD000293 | Pride
Label-free phosphoproteomics 
ProteomicsMultiple protease based human phosphopeptide atlas
Site-specific phospho-antibodies are commonly used to study these changes, however only a limited number of good antibodies is available, covering just a few signaling nodes in important pathways. It is highly unlikely that specific antibodies, covering the more than hundred thousands of estimated p...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-10-04 | MSV000080223 | MassIVE
ProteomicsExtending proteome coverage through an on-line 2D SCX-RP ultra-high pressure system
Ultra high pressure liquid chromatography (UHPLC) systems combined with state of the art mass spectrometers have pushed the limit of deep proteome sequencing to new heights making it possible to identify thousands of proteins in a single LC/MS experiment within a few hours. However, the relationship...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-31 | MSV000080845 | MassIVE
ProteomicsProteomic analysis of T cell differentiation
T helper cells (Th) play an important role guarding, regulating and modulating immune responses. The different Th lineages fulfill different tasks in response to infections. The two best defined subtypes are Th1 (involved in cellular immunity) and Th2 (humoral immunity). The cytokine environment aft...
ORGANISM(S): Mus musculus (Mouse) 
2019-02-14 | PXD004532 | Pride
Th2 Th1 Murine cd4+ t- cells Q exactive Scx 
ProteomicsProbing tyrosine kinase inhibitors specificity
Using a three-pronged MS-based proteomic approach, we assessed the mode-of-action of four clinically-relevant tyrosine kinase inhibitors in an epithelial cancer cell line. Here we defined the: (I) semi-quantitative tyrosine kinome; (II) drug interactome; and (III) drug-induced effects on the tyrosin...
ORGANISM(S): Homo sapiens (Human) 
2014-05-12 | PXD000681 | Pride
Human Tyrosine kinase inhibitors Chemical proteomics Phosphoproteomics Phosphotyrosine Lc-ms/ms 
ProteomicsHs_Phospho_Jurkat_ETD
Ti4+-IMAC phosphopeptide enrichment, ETD, Jurkat T-cells
ORGANISM(S): Homo Sapiens (human) 
2014-12-31 | PAe005124 | PeptideAtlas
ProteomicsHs_Phospho_Hela_CID
Ti4+-IMAC phosphopeptide enrichment, CID, Hela cells
ORGANISM(S): Homo Sapiens (human) 
2014-12-31 | PAe005134 | PeptideAtlas
ProteomicsHs_Phospho_Jurkat_CID
Ti4+-IMAC phosphopeptide enrichment, CID, Jurkat T-cells
ORGANISM(S): Homo Sapiens (human) 
2014-12-31 | PAe005133 | PeptideAtlas
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