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ProteomicsDevelopment of a high-pH reversed-phase well plate for peptide fractionation and deep proteome analysis of cells and exosomes
Development of a high-pH reversed-phase well plate for peptide fractionation and deep proteome analysis of cells and exosomes
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-07-21 | MSV000087860 | MassIVE
ProteomicsIn-depth proteome analysis of human ascites using high-pH peptide fractionation
For in-depth profiling, ascites from patients with benign disease or gastric cancer were subjected to high-resolution mass spectromtery analysis. By high-pH peptide fractionation, 3481 protein groups were identified in 72 LC-MS/MS runs.
ORGANISM(S): Homo sapiens (Human) 
2018-04-16 | PXD003351 | Pride
Ascites High-ph peptide fractionation Gastric cancer 
ProteomicsProteomic analysis of human dentin matrix extracts by peptide-level high-pH reverse-phase fractionation
Proteomics research has increasingly focused on human cells, tissues, and fluids; however, comprehensive data regarding dental tissues remain limited. Dentin, a mineralized component of the tooth, contains structural proteins and bioactive molecules that, upon release, can modulate pulp cell acti...
ORGANISM(S): Homo sapiens (Human) 
2026-03-23 | PXD070849 | Pride
Dentin  dentin matrix  proteomics  extracellular matrix  mass spectrometry  regenerative endodontics 
ProteomicsIPG Strip-Based Peptide Fractionation for Shotgun Proteomics
This deep proteome dataset of human HEK293 cells was generated using IPG Strip-Based fractionation.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-10-21 | MSV000080274 | MassIVE
ProteomicsTrimodal chromatography enables facile peptide fractionation
We evaluated the utility of a trimodal stationary phase (Trinity P1) in offline peptide fractionation, and found it performed similarly to ion exchange or high pH reversed phase based techniques. It is orthogonal to low pH reversed phase and uses MS compatible mobile phase solvents.
ORGANISM(S): Homo sapiens (Human) 
2017-08-07 | PXD006188 | Pride
Peptide fractionation Proteomics Trinity p1 Orthogonality 2d-lc Pancreatic cancer 
ProteomicsMining Missing Membrane Proteins by High-pH Reverse Phase StageTip Fractionation and Validation by Multiple Reaction Monitoring Mass Spectrometry
Several significant efforts have been made in the past decade to map all human proteome. However, more than 3000 proteins are grouped as missing having no evidence of translation. Membrane protein annotation of the current missing proteins list showed 34%to be missing membrane proteins, which are ch...
ORGANISM(S): Homo sapiens (Human) 
2015-07-27 | PXD002224 | Pride
High-ph reverse phase Stagetip Missing proteins Lung cancer 
ProteomicsHuman follicular fluid proteomic and peptidomic composition quantitative studies by SWATH-MS methodology. Applicability of high pH RP-HPLC fractionation
Analysis of proteomic composition of human follicular fluid (hFF) has been proposed as a potential tool of oocyte quality evaluation. In order to develop an efficient method to investigate the hFF proteome and peptidome components, we applied a prefractionation scheme of hFF material consisting of ...
ORGANISM(S): Homo sapiens (Human) 
2018-03-19 | PXD008073 | Pride
Human Follicular fluid Fractionation Swath-ms 
ProteomicsPre-fractionation extends, but creates a bias in the detectable HLA class Ι ligandome
HLA class Ι molecules on the cell surface enable CD8+ T lymphocytes to recognize cellular alterations in the form of antigens, including mutations, protein copy number alterations, aberrant post-translational modifications or pathogen proteins. At any given moment, tens of thousands of different sel...
ORGANISM(S): Homo sapiens (Human) 
2019-02-25 | PXD011257 | Pride
Hla class ι ligandome  hla class ι peptide ligands  high ph reversed phase  strong cation exchange  pre-fractionation 
OtherIPG strip-based peptide fractionation for shotgun proteomics.
Data from ProteomeXchange, PXD ID: PXD002389. Experiment: Bibo_20130621_CHS_IEF_3-10linear_24slices, file: Bibo_20130621_CHS_IEF_3-10linear_24slices_20.mzml. Published as part of Methods Mol Biol. 2014;1156:67-77 . From the Abstract: {{i}} Efficient fractionation of peptides is an essential prerequ...
ORGANISM(S): Homo_sapiens_viruses, Human_female 
GPM32320016255 | GPMDB
Resource Reanalysis 
ProteomicsComparison of fractionation proteomics for local SWATH library building
For data-independent acquisition by means of sequential window acquisition of all theoretical fragment ion spectra (SWATH), a reference library of data-dependent acquisition (DDA) runs is typically used to correlate the quantitative data from the fragment ion spectra with peptide identifications. Th...
ORGANISM(S): Homo sapiens (Human) 
2017-07-04 | PXD006190 | Pride
Library building Comparative study Swath-ms Fractionation proteomics Hesc 
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