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ProteomicsConjugation site characterization of antibody–drug conjugates using electron-transfer/higher-energy collision dissociation (EThcD)
Antibody–drug conjugates (ADCs) are formed by binding of cytotoxic drugs to monoclonal antibodies (mAbs) through chemical linkers. A comprehensive evaluation of the critical quality attributes (CQAs) of ADCs is vital for drug development but remains challenging owing to ADC structural heterogeneity ...
ORGANISM(S): Homo Sapiens 
2023-02-14 | PXD040111 |
UnknownIn-Source Collision-Induced Dissociation (CID) Improves Higher-Energy Collisional Dissociation (HCD)-Dependent Fragmentation of ADP-Ribosyl Peptides.
Not available
S-EPMC11617611 | biostudies-literature
ProteomicsCellular ADP-ribosylome characterization with HCD and EThcD
Protein ADP-ribosylation is a post-translational modification involved in important physiological and pathological processes. Here, we present an optimized method using the Orbitrap Fusion Tribrid mass spectrometer, which drastically improves the overall ADP-ribosylation site localization score and ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-07-28 | MSV000080008 | MassIVE
UnknownDifferentiating Positional Isomers of Nucleoside Modifications by Higher-Energy Collisional Dissociation Mass Spectrometry (HCD MS).
Not available
S-EPMC6062210 | biostudies-literature
ProteomicsMirroring the charged termini; ETD/HCD fragmentation characteristics of LysargiNase and tryptic peptides and their benefits for peptide sequencing in proteomics
A key step in proteomics is the digestion of proteins into peptides, so far largely done by using trypsin. Tryptic digestion leads to peptides that in ESI-MS attain predominantly two charges, via protonation at the free N-terminus and at the C-terminal basic residue Arginine or Lysine. These peptide...
ORGANISM(S): Homo sapiens (Human) 
2016-12-02 | PXD004447 | Pride
Fragmentation Etd Hcd Lysarginase Orbitrap Phosphoproteomics Trypsin 
ProteomicsSequential N/O-glycosylation analysis of heavily glycosylated HIV-1 gp120 by combination of electron-transfer/higher-energy collisional dissociation and stepped collision energy/higher-energy collisional dissociation (EThcD-sceHCD)
The envelope (Env) glycoprotein on the surface of human immunodeficiency virus type 1 (HIV-1) which decorated with a dense array of glycans is a determinant for viral invasion and host immune response of HIV-1 and a major target for a preventive HIV-1 vaccine. Improved vaccine design requires an und...
ORGANISM(S): Homo sapiens (Human) 
2021-11-03 | PXD025078 | Pride
Envelope glycoprotein Human immunodeficiency virus Ethcd-scehcd-ms/ms N/o-glycosylation 
ProteomicsExtensive backbone cleavage coverage of intact proteoforms in a mass range of 10-70 kDa by integrating electron, collision, and photon-based fragmentation techniques during an electrophoretic timescale
Capillary zone electrophoresis (CZE) –-tandem mass spectrometry (MS/MS) has been documented as a useful tool for top-down proteomics (TDP). However, CZE-MS/MS-based TDP typically has limited backbone cleavage coverage for identified proteoforms due to the use of traditional collision-based fragmenta...
ORGANISM(S): Homo sapiens (Human) 
2026-02-16 | PXD070486 | Pride
Capillary zone electrophoresis mass spectrometry  top-down proteomics  orbitrap ascend tribrid  ultraviolet photodissociation  higher-energy collisional dissociation  electron transfer dissociation 
ProteomicsIn-source CID improves HCD-dependent fragmentation of ADP-ribosyl peptides
ADP-ribosylation is a posttranslational modification whose HCD products are dominated by complete or partial modification losses, complicating peptide sequencing and acceptor site localization efforts. We tested whether in-source CID performed on a quadrupole Orbitrap could convert ADPr to the small...
ORGANISM(S): Homo sapiens (Human) 
2024-12-05 | PXD055586 | Pride
Sid Proteomics Hcd Exploris480 Adp-ribosylation 
ProteomicsOptimal Dissociation Methods Differ for N- and O-glycopeptides
Site-specific characterization of glycosylation requires intact glycopeptide analysis, and recent efforts have focused on how to best interrogate glycopeptides using tandem mass spectrometry (MS/MS). Beam-type collisional activation, i.e., higher-energy collisional dissociation (HCD), has been a val...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2020-07-20 | PXD017646 | Pride
Glycoproteomics N-glycopeptides Scehcd Ethcd O-glycopeptides Etd 
ProteomicsEThcD-sceHCD-MS/MS-based Clinical N-glycoproteomics
Site-specific N-glycosylation characterization requires intact N-glycopeptide analysis based on suitable tandem mass spectrometry (MS/MS) method. Electron-transfer/higher-energy collisional dissociation (EThcD), stepped collision energy/higher-energy collisional dissociation (sceHCD), and higher-ene...
ORGANISM(S): Homo sapiens (Human) 
2022-04-04 | PXD030288 | Pride
Glycoproteomics Mass spectrometry Ethcd-scehcd Fragmentation mode N-glycosylation Clinical sample 
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