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TranscriptomicsA Basic Domain in the Histone H2B N-terminal Tail is Important for Nucleosome Assembly by FACT
Nucleosome assembly in vivo requires assembly factors, such as histone chaperones, to bind to histones and mediate their deposition onto DNA. In yeast, the essential histone chaperone FACT (FAcilitates Chromatin Transcription) functions in nucleosome assembly and H2A-H2B deposition during transcript...
ORGANISM(S): Saccharomyces cerevisiae 
2016-08-10 | E-GEOD-80831 | biostudies-arrayexpress
GenomicsDistribution of active (H3K4me1, H3K4me3, H3K27ac) and inactive (H3K9me3, H3K27me3) histone PTMs in WT and HIRA KO HeLa cells
We profiled the enrichment of active (H3K4me1, H3K4me3, H3K27ac) and inactive (H3K9me3, H3K27me3) histone PTMs in cells that are wild-type (WT) or knock-out (KO) for the H3.3-specific chaperone HIRA. We performed native ChIP-seq following MNase digestion to isolate nucleosomes in H3.1-SNAP and H3.3-...
ORGANISM(S): Homo sapiens 
2025-08-07 | E-MTAB-14416 | biostudies-arrayexpress
TranscriptomicsHIRA, a Conserved Histone Chaperone, Plays an Essential Role in Low-dose Stress Response via Transcriptional Stimulation in Fission Yeast
Cells that have been pre-exposed to mild stress (priming stress) acquire transient resistance to subsequent severe stress even under different combinations of stresses. This phenomenon is called cross-tolerance. Although it has been reported that cross-tolerance occurs in many organisms, the molecul...
ORGANISM(S): Schizosaccharomyces pombe 
2012-07-21 | E-GEOD-35281 | biostudies-arrayexpress
TranscriptomicsRNA-seq of HeLa cells to investigate regulation of replicative histone RNA metabolism by the histone chaperone ASF1
Histones are the protein components of the basic unit of chromatin, the core particle of the nucleosome. They play a central role in defining chromatin states associated with distinct cell fates and are classified into replicative and non-replicative/replacement histone variants. While the latter do...
ORGANISM(S): Homo sapiens 
2023-11-29 | E-MTAB-12501 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of hip1::ura4+ and slm9::ura4+ S. pombe to investigate the role of the HIRA histone chaperone complex
The assembly of nucleosomes by histone chaperones is an important component of transcriptional regulation. Here we have assessed the global roles of the S. pombe HIRA histone chaperone complex. Microarray analysis indicates that inactivation of the HIRA complex results in increased expression of at ...
ORGANISM(S): Schizosaccharomyces pombe 
2009-07-22 | E-MTAB-130 | biostudies-arrayexpress
GenomicsGenome-wide maps of WT and over-expressing CenH3/CENP-A in Human HeLa S3 cells
Centromeres are essential to ensure proper chromosome segregation in eukaryotes. Their definition relies on the presence of a centromere-specific H3 histone variant CenH3, known as CENP-A in mammals. Its overexpression in aggressive cancers raises questions concerning its effect on chromatin dynamic...
ORGANISM(S): Homo sapiens 
2014-02-12 | E-GEOD-42951 | biostudies-arrayexpress
GenomicsAnp32e is a histone chaperone that removes H2A.Z from chromatin
We report a genome wide enrichment, redistribution and accumulation of H2A.Z at specific chromatin control regions, in particular at enhancers and insulators, in mouse embryonic fibroblasts depleted for Anp32e (MEFs Anp32e-/-). H2A.Z ChIP-seq in MEFs WT (+/+) or KO (-/-) for Anp32e.
ORGANISM(S): Mus musculus 
2014-01-22 | E-GEOD-51579 | biostudies-arrayexpress
TranscriptomicsEffect of a cardiomyocyte-specific Hira conditional knockout on gene expression in the heart
We performed gene expression profiling by microarray using RNA extracted from healthy free wall left ventricle tissues from control and Hira cardiomyocyte-specific conditional knockout mice at 6 weeks of age. Hira is a histone chaperone responsible for replication-independent incorporation of histo...
ORGANISM(S): Mus musculus 
2016-04-06 | E-GEOD-71833 | biostudies-arrayexpress
ProteomicsDAXX adds a de novo H3.3K9me3 deposition pathway to the histone chaperone network (part 1)
A multitude of histone chaperones are required to support histones from their biosynthesis until DNA deposition. They cooperate through the formation of histone co-chaperone complexes, but the crosstalk between nucleosome assembly pathways remains enigmatic. Using exploratory interactomics, we defin...
ORGANISM(S): Homo sapiens (Human) 
2023-03-02 | PXD034888 | Pride
Asf1 Nucleosome assembly Epigenetics Hjurp Nasp Histone chaperone Gene silencing Heterochromatin Daxx 
GenomicsHistone chaperone HIRA orchestrates H4K16ac-decorated dynamic chromatin in senescent cells and is required for suppression of oncogene-induced neoplasia.
Cellular senescence is a stable proliferation arrest that suppresses tumorigenesis. Histone chaperone HIRA deposits nucleosome-destabilizing histone variant H3.3 into chromatin in a DNA replication-independent manner. Histone H3.3 and a subset of other typically M-bM-^@M-^\replication-dependentM-bM-...
ORGANISM(S): Homo sapiens 
2014-11-26 | E-GEOD-56307 | biostudies-arrayexpress
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