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TranscriptomicsTranscription profiling of hip1::ura4+ and slm9::ura4+ S. pombe to investigate the role of the HIRA histone chaperone complex
The assembly of nucleosomes by histone chaperones is an important component of transcriptional regulation. Here we have assessed the global roles of the S. pombe HIRA histone chaperone complex. Microarray analysis indicates that inactivation of the HIRA complex results in increased expression of at ...
ORGANISM(S): Schizosaccharomyces pombe 
2009-07-22 | E-MTAB-130 | biostudies-arrayexpress
TranscriptomicsHIRA, a Conserved Histone Chaperone, Plays an Essential Role in Low-dose Stress Response via Transcriptional Stimulation in Fission Yeast
Cells that have been pre-exposed to mild stress (priming stress) acquire transient resistance to subsequent severe stress even under different combinations of stresses. This phenomenon is called cross-tolerance. Although it has been reported that cross-tolerance occurs in many organisms, the molecul...
ORGANISM(S): Schizosaccharomyces pombe 
2012-07-21 | E-GEOD-35281 | biostudies-arrayexpress
TranscriptomicsA Basic Domain in the Histone H2B N-terminal Tail is Important for Nucleosome Assembly by FACT
Nucleosome assembly in vivo requires assembly factors, such as histone chaperones, to bind to histones and mediate their deposition onto DNA. In yeast, the essential histone chaperone FACT (FAcilitates Chromatin Transcription) functions in nucleosome assembly and H2A-H2B deposition during transcript...
ORGANISM(S): Saccharomyces cerevisiae 
2016-08-10 | E-GEOD-80831 | biostudies-arrayexpress
ProteomicsStructure of the Hir histone chaperone complex
The evolutionarily conserved HIRA/Hir histone chaperone complex and ASF1/Asf1 co-chaperone cooperate for replication-independent chromatin assembly. Here we report the molecular architecture of the Hir complex with Asf1/H3/H4 via single-particle cryo-EM and crosslinking mass spectrometry.
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2024-08-22 | PXD040705 | Pride
Histone Deposition Xlms Em Dna Chaperone Rna Yeast 
TranscriptomicsRNA-seq of HeLa cells to investigate regulation of replicative histone RNA metabolism by the histone chaperone ASF1
Histones are the protein components of the basic unit of chromatin, the core particle of the nucleosome. They play a central role in defining chromatin states associated with distinct cell fates and are classified into replicative and non-replicative/replacement histone variants. While the latter do...
ORGANISM(S): Homo sapiens 
2023-11-29 | E-MTAB-12501 | biostudies-arrayexpress
GenomicsAnp32e is a histone chaperone that removes H2A.Z from chromatin
We report a genome wide enrichment, redistribution and accumulation of H2A.Z at specific chromatin control regions, in particular at enhancers and insulators, in mouse embryonic fibroblasts depleted for Anp32e (MEFs Anp32e-/-). H2A.Z ChIP-seq in MEFs WT (+/+) or KO (-/-) for Anp32e.
ORGANISM(S): Mus musculus 
2014-01-22 | E-GEOD-51579 | biostudies-arrayexpress
TranscriptomicsExpression profiles of the NuB4/HAT-B complex and histone knockouts
Dynamic modification of histone proteins plays a key role in regulating gene expression. However, histones themselves can also be dynamic, which potentially affects the stability of histone modifications. To determine the molecular mechanisms of histone turnover we developed a parallel screening met...
ORGANISM(S): Saccharomyces cerevisiae 
2012-05-14 | E-GEOD-30168 | biostudies-arrayexpress
ProteomicsThe CENP-T/-W complex is a binding partner of the histone chaperone FACT
The CENP-T/-W histone fold complex, as an integral part of the inner kinetochore, is essential for building a proper kinetochore at the centromere in order to direct chromosome segregation during mitosis. Notably, CENP-T/-W is not inherited at centromeres and new deposition is absolutely required at...
ORGANISM(S): Homo sapiens (Human) 
2016-06-14 | PXD004099 | Pride
Centromere Cenp Mitosis Histone chaperone 
GenomicsGenome-wide maps of WT and over-expressing CenH3/CENP-A in Human HeLa S3 cells
Centromeres are essential to ensure proper chromosome segregation in eukaryotes. Their definition relies on the presence of a centromere-specific H3 histone variant CenH3, known as CENP-A in mammals. Its overexpression in aggressive cancers raises questions concerning its effect on chromatin dynamic...
ORGANISM(S): Homo sapiens 
2014-02-12 | E-GEOD-42951 | biostudies-arrayexpress
TranscriptomicsEffect of a cardiomyocyte-specific Hira conditional knockout on gene expression in the heart
We performed gene expression profiling by microarray using RNA extracted from healthy free wall left ventricle tissues from control and Hira cardiomyocyte-specific conditional knockout mice at 6 weeks of age. Hira is a histone chaperone responsible for replication-independent incorporation of histo...
ORGANISM(S): Mus musculus 
2016-04-06 | E-GEOD-71833 | biostudies-arrayexpress
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