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ProteomicsBLM helicase protein negatively regulates stress granule formation through unwinding RNA G-quadruplex structures
We ran native 5% polyacrylamide-TBE gel for electro mobility shift assay (EMSA) for DNA/RNA G4 structures with or without recombinant human core-BLM (cBLM) protein, which was extracted and purified from E.coli in order to show binding of the RNA/DNA by this protein. To further evidence, we cut the e...
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD042983 | Pride
Blm Sg Emsa G-quadruplex Lc-ms/ms 
TranscriptomicsComparison of gene expression profiles of miR-142-/- primary megakaryocytes and WT primary megakaryocytes
Whole fetal livers were collected from mouse fetuses at embryonic day 14.5 (E14.5), and single-cell suspensions were prepared by successive passage through 18-, 21 and 23-gauge needles. Fetal liver cells were maintained in Dulbecco modified Eagle medium (DMEM; Invitrogen) supplemented with 10% feta...
ORGANISM(S): Mus musculus 
2014-06-11 | E-GEOD-52141 | biostudies-arrayexpress
ProteomicsUbiquitination regulates granulostasis and DRiP accumulation in SGs under heat stress via the E3 ligase MKRN2
Stress granules (SGs) are transient cytoplasmic biomolecular condensates that play a role in the cellular response to proteotoxic stress. It has been previously shown that ubiquitination regulates SG dynamics; however, the specific mechanisms by which ubiquitin affects SGs are not fully understood. ...
ORGANISM(S): Homo sapiens (Human) 
2026-09-09 | PXD076622 | Pride
Stress granules ubiquitin 
ProteomicsmiR-17-92/106b regulate beta cell mitotic checkpoint and insulin secretion
Adult beta cells in the pancreas are the sole source of insulin in our body. Beta cell loss or increased demand for insulin, impose metabolic challenges because adult beta cells are generally quiescent and infrequently re-enter the cell division cycle. miR-17-92/106b is a family of proto-oncogene mi...
ORGANISM(S): Mus musculus (Mouse) 
2019-06-17 | PXD012610 | Pride
Mouse Lc-msms Mir-17-92/106b 
TranscriptomicsMicroRNA expression in the newborn mouse cochlea and vestibule
MicroRNAs (miRNAs) inhibit the translation of target mRNAs and affect, directly or indirectly, the expression of a large portion of the protein-coding genes. This study focuses on miRNAs that are expressed in the mouse cochlea and vestibule, the two inner ear compartments. To identify miRNAs that ar...
ORGANISM(S): Mus musculus 
2010-06-20 | E-GEOD-15496 | biostudies-arrayexpress
ProteomicsBLM helicase protein negatively regulates stress granule formation through unwinding RNA G-quadruplex structures
Bloom’s syndrome (BLM) protein is a known nuclear helicase that is able to unwind DNA secondary structures such as G-quadruplexes. However, its role in the regulation of cytoplasmic processes that involve RNA G-quadruplexes (rG4s) has not been previously studied. Here, we demonstrate that BLM is rec...
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD039534 | Pride
Stress granule Apex Helicase Lc-msms G-quadruplex Rg4-binding proteins Blm Quma-1 
UnknownThe E3 ligase MKRN2 prevents DRiP accumulation in stress granules and maintains granulostasis
Not available
S-SCDT-10_1038-S44319-026-00824-2 | biostudies-other
UnknownThe E3 ligase MKRN2 prevents DRiP accumulation in stress granules and maintains granulostasis
Not available
S-SCDT-10_1038-S44319-026-00832-2 | biostudies-other
Transcriptomics3'RNA-seq of mouse neuronal cell-line NSC-34 treated with siAGO2 or siNT (non-targeting) as control
Exploring the involvement of AGO2 in regulating alternative polyadenylation, by analyzing all 3' end transcript variants using 3'RNA-seq data.
ORGANISM(S): Mus musculus 
2021-12-06 | E-MTAB-9907 | biostudies-arrayexpress
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