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ProteomicsTIHAMAC: Streamlined Tandem IMAC-HILIC Workflow for Simultaneous and High-Throughput Plant Phosphoproteomics and N-glycoproteomics Analysis
Protein post-translational modifications (PTMs) are pivotal molecular events in plant cells, encompassing processes such as protein folding and enzyme activation. Among these PTMs, N-glycosylation and phosphorylation hold particular importance, as they play essential roles in regulating plant growth...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-04-03 | PXD047065 | Pride
N-glycoproteomics Hilic Arabidopsis thaliana Imac Aba signaling Phosphoproteomics 
ProteomicsA Suspension Trapping-based Sample Preparation Workflow for Sensitive Plant Phosphoproteomics
Plant phosphoproteomics reveals a global view of phosphorylation-mediated signaling in plants, but it remains demanding a high-throughput method with detection sensitivity and quantification accuracy. In general, the indispensable protein precipitation step requiring for interferences removal limite...
ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress) 
2023-08-28 | PXD039933 | JPOST Repository
ProteomicsIntegrated Single-Tip IMAC-HILIC Enrichment Enables Simultaneous and Efficient Analysis of Plant Phosphoproteomics and N-glycoproteomics
Protein phosphorylation and N-glycosylation are key post-translational modifications (PTMs) in plants that regulate critical signaling processes. However, analyzing both PTMs simultaneously remains challenging due to low abundance and divergent enrichment requirements. Here, we present a single-tip ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2025-05-14 | PXD061247 | Pride
Phosphoproteomics  n-glycoproteomics  enrichment  immobilized metal affinity chromatography  hydrophilic interaction chromatography 
ProteomicsHigh-throughput Phosphorylation Screening and Validation through Ti(IV)-Nanopolymer Functionalized Reverse Phase PhosphoProtein Array
Protein phosphorylation is one of the most important and widespread molecular regulatory mechanisms that controls almost all aspects of cellular functions in animals and plants. Here, we introduce a novel chemically functionalized Reverse Phase PhosphoProtein Array (RP3A) to capture and measure phos...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2019-11-12 | PXD010208 | Pride
Phosphorylation 
ProteomicsUstilago maydis PR-1-like protein has evolved two distinct domains for dual virulence activities
Diversification of effector function, driven by a co-evolutionary arms race, enables pathogens to establish compatible interactions with hosts. Structurally conserved plant pathogenesis-related PR-1 and PR-1-like (PR-1L) proteins are involved in plant defense and fungal virulence, respectively. It i...
ORGANISM(S): Ustilago maydis (Smut fungus) 
2023-08-29 | PXD044915 | Pride
Ustilago maydis Cape-like Fungal virulence Cathepsin b-like 3 Pry1 Phenolic resistance Pr-1-like Cnyd motif 
ProteomicsMapping Proteome-Wide Targets of Protein Kinases in Response to Stresses
Protein kinases are major regulatory components in almost all cellular processes in eukaryotic cells. By adding phosphate groups, protein kinases regulate the activity, localization, protein-protein interactions and other profiles of their target proteins. It is known that protein kinases such as SN...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2020-01-16 | PXD010053 | Pride
Phosphoproteomics Plant kinase Stress 
ProteomicsDIA-based phosphoproteomics identifies early phosphorylation events in response to EGTA and mannitol in Arabidopsis
The rapid activation of RAF-SnRK2 cascades in response to hyperosmolarity is a crucial event in plants' adaptation to osmotic stress, yet the underlying mechanisms have remained elusive. In this study, we reveal that the calcium chelator EGTA, but not other calcium modulators, can potently activate ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-04-18 | PXD046802 | Pride
Arabidopsis Dia Phosphoproteomics Egta 
ProteomicsUnveiling previously undescribed circadian clock regulators and action mechanisms via TurboID-based profiling of the LWD1 interactome in Arabidopsis thaliana
The synchronization of the internal circadian clock with the external 24-h diurnal cycle is critical for ensuring optimal plant growth fitness. Despite lacking a DNA binding domain, LIGHT-REGULATED WD proteins (LWDs) are essential for sustaining and fine-tuning circadian rhythm1,2. However, the acti...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2026-07-21 | PXD062258 | Pride
Circadian clock Photomorphogenesis Turboid Proximality labeling Photoperiodic flowering Lwd1 
ProteomicsOK-SnRK2 cascade mediates osmotic stress signaling in plant
Osmotic stress associated with drought, salinity and cold is a major factor limits plant productivity. A few signaling factors have been identified in the sensing or early signaling of osmotic stress. SNF1-related protein kinase 2 (SnRK2) are quickly activated by osmotic stress and the snrk2 decuple...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2020-01-17 | PXD014435 | Pride
Snrk2 Osmotic stress active protein kinases Osmotic stress 
ProteomicsReciprocal regulation of the TOR kinase and ABA receptor balances plant growth and stress response
We found that TOR kinase regulates the plant environmental stress responses through phosphorylation at a conserved serine residue of Abscisic acid receptor PYR1/PYLs/RCARs. The phosphorylation inhibits the function of PYLs and prevents the binding of PYLs to abscisic acid and its downstream substrat...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2017-12-01 | PXD003746 | Pride
Snrk2 Aba receptor Raptor Abscisic acid Target of rapamycin Phosphorylation 
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