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ProteomicsEpitope Mapping of Human Polyclonal Antibodies to the fHbp Antigen of a Neisseria Meningitidis Vaccine by Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS)
Antigen-antibody interactions play a key role in the immune response post vaccination and the mechanism of action of antibody-based biopharmaceuticals. 4CMenB is a multicomponent vaccine against Neisseria meningitidis serogroup B in which factor H binding protein (fHbp) is one of the key antigens. I...
ORGANISM(S): Homo sapiens (Human) 
2024-05-24 | PXD046572 | Pride
Polyclonal antibodies Pab Fhbp Hdx-ms 
ProteomicsEpitope Mapping of Human Polyclonal Antibodies to the fHbp Antigen of a Neisseria Meningitidis Vaccine by Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS) - part 2
Antigen-antibody interactions play a key role in the immune response post vaccination and the mechanism of action of antibody-based biopharmaceuticals. 4CMenB is a multicomponent vaccine against Neisseria meningitidis serogroup B in which factor H binding protein (fHbp) is one of the key antigens. I...
ORGANISM(S): Homo sapiens (Human) 
2024-05-22 | PXD047712 | Pride
Pab Fhbp Hdx-ms 
ProteomicsThe Deuterium Calculator HDX-MS Data Analysis
Hydrogen-deuterium exchange mass spectrometry (HDX-MS) is a powerful protein footprinting technique to study protein dynamics and binding; however, HDX-MS data analysis is often challenging and time consuming. Moreover, the HDX community is expanding to investigate multi-protein and highly complex p...
ORGANISM(S): Escherichia coli 
2023-01-30 | PXD036813 | Pride
Hydrogen-deuterium exchange mass spectrometry Subzero temperature uplc Hdx data analysis software Hdx-ms 
ProteomicsHuman Calcitonin and Amylin receptor HDX-MS
Receptor activity-modifying proteins (RAMPs) regulate the pharmacology, trafficking, and signaling of class B1 G protein-coupled receptors (GPCRs). The calcitonin receptor (CTR) interacts with each of the three RAMPs to form amylin receptors (AMYRs), which display distinct ligand preferences and sig...
ORGANISM(S): Homo sapiens (Human) 
2026-06-08 | PXD068848 | Pride
Calcitonin recepto Hdx-ms Recepror activity modifying protein (ramp) Gpcr Amylin receptor 
ProteomicsDetermination of highly accurate small molecule:protein affinity constants at amino acid resolution using Hydrogen Deuterium Exchange Mass Spectrometry (HDX-MS)
Hydrogen Deuterium Exchange Mass Spectrometry (HDX-MS) has been widely adopted as part of the drug development pipeline, frequently used in both the development of biopharmaceuticals and small molecule compound development to assess target engagement spatially. Frequently, HDX-MS analyses are conduc...
ORGANISM(S): Plasmodium falciparum 58.1 
2026-04-01 | PXD070051 | Pride
ProteomicsSubzero hydrogen-deuterium exchange CE-MS
We have performed in solution hydrogen exchange (HDX) on peptide standards and bovine hemoglobin with subsequent quenching, pepsin digestion, and cold capillary electrophoretic separation coupled with mass spectrometry (MS) detection for the benchmarking of a lab built HDX-MS platform. Though neutra...
ORGANISM(S): Bos taurus (Bovine) 
2022-12-22 | PXD034245 | Pride
Ce-ms Hdx Hemoglobin 
ProteomicsComplementary Backbone and Side-chain Analysis of Drug – Protein Interactions: Combining Hydrogen/Deuterium Exchange and Protein Oxidative Footprinting to Discriminate the Binding of Small Molecule Therapeutics
Characterizing and distinguishing protein-ligand interactions is crucial for understanding cellular metabolism and guiding drug discovery and development. We employ hydrogen/deuterium exchange mass spectrometry (HDX-MS) and a new Fenton chemistry-based approach to protein oxidative mass spectrometry...
ORGANISM(S): Homo sapiens (Human) 
2026-03-27 | PXD050663 | Pride
Hdx Ox-ms Bcl2 Binding 
ProteomicsSimple and Fast Maximally Deuterated Control (maxD) preparation for HDX MS Experiments
During the analysis steps of hydrogen deuterium exchange (HDX) mass spectrometry (MS) there is an unavoidable loss of deuterons, or back-exchange. Understanding back-exchange is necessary to correct for loss during analysis, to calculate the absolute amount of exchange, and to ensure that deuterium ...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) Equus caballus (Horse) 
2022-07-08 | PXD032924 | Pride
Hdx ms Mass spectrometry Hydrogen deuterium exchange Back-exchange Maximally deuterated samples 
ProteomicsInvestigation of GPCR-G protein coupling by Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS) : the GPBAR1 case.
G protein-coupled receptors (GPCRs) are key classes of proteins for drug discovery research as many of them are still orphans and their function poorly known. Their activation mechanism is roughly divided in two states : active and unactive states however related dynamics to switch from one state to...
ORGANISM(S): Homo sapiens (Human) 
2025-09-08 | PXD047827 | Pride
Cryo-em Gpbar1 G protein Gpcr-g protein Tgr5 Hdx-ms Gpcr 
ProteomicsComparative analysis of the structural dynamics of diphtheria toxin and CRM197 carrier proteins used in the development of conjugate vaccines
Carrier proteins are chemically linked to poorly immunogenic antigens to generate conjugate vaccines, which have significantly improved immunization strategies in recent decades. CRM197, a genetically detoxified diphtheria toxin (DT) mutant carrying the G52E mutation, is a widely used carrier protei...
ORGANISM(S): Escherichia coli Corynebacterium diphtheriae 
2025-04-07 | PXD057388 | Pride
Comparative analysis Crm197 Structural characterization Hydrogen/deuterium exchange mass spectrometry (hdx-ms) Biopharmaceutical products 
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