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ProteomicsGetting more out of FLAG-Tag co-immunoprecipitation mass spectrometry experiments using FAIMS.
Co-immunoprecipitation of proteins coupled to mass spectrometry is critical for the understanding of protein interaction networks. In instances where a suitable antibody is not available, it is common to graft synthetic tags onto target protein sequences and allowing the use of commercially availabl...
ORGANISM(S): Homo sapiens (Human) 
2023-02-21 | PXD028965 | Pride
Faims Co-ip mass spectrometry Flag tag Proteomics Ion mobility 
UnknownAutomated Immunoprecipitation, Sample Preparation, and Individual Ion Mass Spectrometry Platform for Proteoforms.
Not available
S-EPMC11825902 | biostudies-literature
UnknownRapid immunoprecipitation mass spectrometry of endogenous protein (RIME) to identify chromatin-interactome in prostate cancer cells.
Not available
S-EPMC9184806 | biostudies-literature
UnknownA Spectrum of Neural Autoantigens, Newly Identified by Histo-Immunoprecipitation, Mass Spectrometry, and Recombinant Cell-Based Indirect Immunofluorescence.
Not available
S-EPMC6046535 | biostudies-literature
ProteomicsNovel autoantibodies in rheumatoid arthritis using immunoprecipitation coupled with mass spectrometric analysis.
Rheumatoid arthritis (RA) is a systemic autoimmune disease characterized by chronic joint inflammation resulting in progressive cartilage and bone destruction. In two thirds of early RA patients, rheumatoid factor (RF) and/or anti-citrullinated protein antibodies (ACPAs) are present. While these ant...
ORGANISM(S): Homo sapiens (Human) 
2026-04-27 | PXD069628 | Pride
Anti nuclear antibodies Mass spectrometry Ipms Rheumatoid arthritis Autoantibodies 
Proteomicsimmunoprecipitation coupled with a mass spectrometry (MS) approach to identify cellular proteins associated with MOV10
To understand the detailed mechanism underlying MOV10-mediated restriction of LINE-1 retrotransposition, we first performed immunoprecipitation coupled with a mass spectrometry (MS) approach to identify cellular proteins associated with MOV10.
ORGANISM(S): Homo sapiens (Human) 
2023-08-30 | PXD041814 | Pride
Mass，mov10 Ip 
TranscriptomicsIdentification of differentially methylated regions between placentas and maternal blood cells on chromosome 18
Comparison of DNA methylation levels of chromosome 18 between placentas and blood cells of pregnant women Extracted DNA from 5 cases of placental tissues and 5 cases of maternal blood cell was subjected to MeDIP (methylated DNA immunoprecipitation) and hybridized to Affymetrix GeneChip® Human Tiling...
ORGANISM(S): Homo sapiens 
2010-12-22 | E-GEOD-22837 | biostudies-arrayexpress
ProteomicsGlycine receptor complex analysis using immunoprecipitation-blue native gel electrophoresis-mass spectrometry
The pentameric glycine receptor (GlyR), comprising the α1 and β subunits, is a major inhibitory ionotropic receptor in brainstem and spinal cord. GlyRs interact with gephyrin (GPHN), a scaffold protein that anchors the GlyR in the plasma membrane and enables it to form clusters in glycinergic postsy...
ORGANISM(S): Mus musculus (Mouse) 
2022-12-12 | PXD017151 | Pride
Blue native gel electrophoresis Proteomics Protein complex Glycine receptor Iqsec3 
TranscriptomicsAnalyses of cyclin D1 function using a "genetic-proteomic" approach
We examined the transcriptional function of cyclin D1 in mouse development using two approaches. First, we queried association of cyclin D1 with the genome of E14.5 mouse embryos using ChIP-on-chip approach. We observed binding of cyclin D1 to several promoter regions. Second, we compared gene exp...
ORGANISM(S): Mus musculus 
2010-01-28 | E-GEOD-13636 | biostudies-arrayexpress
GenomicsChromatin profiling reveals novel proteins associated with histone-marked genomic regions
Our understanding of the functions of DNA elements is limited by the paucity of information about the spectrum of proteins that occupy these genomic regions. Here we describe an approach to identify proteins associated with genomic regions whose chromatin is marked by specific modified histones, whi...
ORGANISM(S): Mus musculus 
2015-02-05 | E-GEOD-62380 | biostudies-arrayexpress
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